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Nanoparticles prepared by applying microfluidic technology, preparation method and device and application

A microfluidic technology and nanoparticle technology are applied in the fields of preparing nanoparticles by applying microfluidic technology, preparation, devices and uses, which can solve the problems of unstable system, easy carrier material, low dispersibility, etc., and achieve good dispersibility. stability, good biocompatibility, and broad application prospects

Active Publication Date: 2021-09-24
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the common disadvantages of these methods are: 1. The preparation takes a long time, the carrier material is easy to degrade, and there are many experimental uncertainties
2. The system is unstable, the dispersion is not high, the encapsulation efficiency is low, and the drug loading efficiency needs to be improved
3. Some clathrate carriers have not been approved for food applications

Method used

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  • Nanoparticles prepared by applying microfluidic technology, preparation method and device and application
  • Nanoparticles prepared by applying microfluidic technology, preparation method and device and application
  • Nanoparticles prepared by applying microfluidic technology, preparation method and device and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Construction of a glass microfluidic device

[0050] The glass microfluidic device is a single-emulsion device prepared by a self-made one-step method, including an inner phase capillary 1, an outer phase capillary 3, a square tube 2, a sampling needle 4, two iron tubes 5 and a glass slide 6. Use a needle pulling device to burn a long capillary into two to three sections, and then use a needle grinder to burn and pull the needle pulling device to prepare the two capillary tips required for the experiment to be polished to 40-60μm and 200-400μm respectively. , as inner phase capillary 1 and outer phase capillary 3. Specifically, place the two polished capillaries on the observation platform of an inverted microscope, and observe whether the thickness of the tips of the two capillaries meets the experimental requirements through the microscope eyepiece and the JIFEI microscope-specific measurement software on the computer side connected to the microscope. Firs...

Embodiment 2

[0051] Embodiment 2: the preparation of nanoparticle

[0052] (1) Preparation of two-phase solution

[0053] Weigh a part of CAPE (0.01-0.1% w / v) and shellac (0.5-4.0% w / v) and dissolve in 1ml of ethanol, place on a magnetic stirrer and stir until completely dissolved (if the stirring is not completely dissolved, use a vortex Mixer to aid in dissolving) to prepare the inner aqueous phase solution, dissolve gum arabic (0-2.5% w / v) in deionized water, place the solution on a vortex mixer to quickly dissolve the gum arabic, and obtain the outer aqueous phase Solution, wherein the ratio of ethanol to water is (1:5, 1:10, 1:15 and 1:20).

[0054] (2) Preparation of nanoparticles

[0055] Inhale the inner aqueous phase solution into a 1ml disposable sterile medical syringe, and at the same time use a 5ml disposable sterile medical syringe to absorb the outer aqueous phase solution, fix the inner phase and outer phase syringes on two syringe pumps respectively, The tetrafluoroethy...

Embodiment 3

[0059] Example 3: Characterization of Nanoparticles

[0060] (1) Characterization of the particle size of the nanoparticles: the effect of the used material parameters on the particle size and potential of the nanoparticles was investigated.

[0061] Under the condition that the above-mentioned conditions for the preparation of nanoparticles remain unchanged, the effects of the concentration of gum arabic, the concentration of shellac, and the ratio of solvent to antisolvent on the particle size and potential of nanoparticles were investigated respectively, and the particle size was measured by Anton Paar laser particle size analyzer (Litesizer500). Particle Size Distribution and Zeta Potential.

[0062] Discussion of results: attached Figure 4 , 5 , 6, through different groups of single factor experiments, it was found that the particle size and potential measurement results of nanoparticles prepared with different concentration groups of gum arabic concentration showed th...

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Abstract

The invention discloses nanoparticles prepared by applying a microfluidic technology, a preparation method and device and application of the nanoparticles. The particle size of the nanoparticles is 175nm-225nm, the particle size dispersity is 15%-30%, and the electric potential is -25MV to -5MV. The method comprises the following steps: completely dissolving shellac in a polar solvent, and then, dissolving a medicine CAPE in the polar solvent to prepare an internal phase solution; dissolving Arabic gum in deionized water to prepare an external water phase solution; wrapping the internal phase solution in the external water phase solution, and preparing a nanoparticle solution by adopting a one-step method device; and carrying out rotary evaporation on the nanoparticle solution to remove the solvent from the solution, and carrying out drying, thereby obtaining the product. The nanoparticles prepared by the invention are loaded with the medicine CAPE and have good dispersibility and stability, the structure and activity of the encapsulated CAPE are protected in a stomach, and the encapsulated CAPE is specifically released in an intestinal tract region.

Description

technical field [0001] The invention relates to nanoparticles, a preparation method and a device, in particular to the application of microfluidic technology to prepare nanoparticles, a preparation method, a device and an application. Background technique [0002] Caffeic acid phenethyl ester (CAPE) is a biologically active flavonoid compound extracted from propolis, and its structure contains 0-dihydroxy (catechol) phenyl structure. CAPE has a wide range of biological properties. Because of its unique physiological and pharmacological effects in anti-tumor, anti-oxidation, anti-inflammatory and antibacterial activities, it has become a hot spot in the research of propolis active ingredients. However, the solubility of CAPE is very low, its water solubility is poor, and its biological performance is poor. It has been proved that CAPE is easily decomposed by plasma enzymes in rat plasma and hydrolyzed into caffeic acid, resulting in shortened half-life, which limits their use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/52A61K47/46A61K31/216A61P35/00A61P39/06A61P29/00A61P31/04A61P31/10B01J19/00A23L21/20A23L29/25A23L29/275A23L33/00A23P10/30
CPCA61K9/5192A61K9/5123A61K31/216A61P35/00A61P39/06A61P29/00A61P31/04A61P31/10B01J19/0093A23L33/00A23L21/20A23P10/30A23L29/275A23L29/25A23V2002/00A23V2250/204A23V2250/5028A23V2250/502Y02A50/30
Inventor 王志祥王新王凯叶
Owner CHINA PHARM UNIV
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