Kit and method for detecting aminoglycoside drug-induced deafness-sensitive gene

Abstract

The invention relates to the field of gene detection, and particularly relates to a kit and method for detecting an aminoglycoside drug-induced deafness-sensitive gene through a fluorescent PCR (polymerase chain reaction)-melting curve method. The technical scheme of the invention provides a kit for detecting an aminoglycoside drug-induced deafness-sensitive gene. The kit comprises a PCR solution, a negative quality control sample and a positive quality control sample, wherein the PCR solution comprises primers, a PCR Buffer, a dNTP (deoxyribonucleotide triphosphate) mixed solution, saturated fluorescent dyes, a Taq enzyme and a UNG enzyme. In the invention, the specific PCR primers are designed according to SNP (single nucleotide polymorphism) sites of the aminoglycoside drug-induced deafness-sensitive gene, two aminoglycoside drug-induced deafness-sensitive sites can be detected in one PCR, the operation can be finished in one tube, and the detection is quick, accurate and wide in range.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a kit and a method for detecting aminoglycoside drug-induced deafness susceptibility genes by fluorescent PCR-melting curve method. Background technique [0002] According to my country's second national sampling survey of disabled people in 2006, there were 20.04 million people with hearing disabilities, accounting for 24.16% of the country's 82.96 million disabled people. Among them, drug-induced deafness accounted for 30%-40%, and the number reached 8 million. Drug-induced deafness refers to the deafness caused by the use of certain drugs to treat diseases or the exposure of the human body to certain chemical agents. More than 100 ototoxic drugs have been found so far, and ototoxic antibiotics are the most common. Aminoglycoside antibiotics are the most common ototoxic antibiotics, including streptomycin and gentamicin commonly used in clinical practice. In the past ten years, ...

AI Technical Summary

Problems solved by technology

[0009] Disadvantages common to common PCR products: the genotype needs to be judged by the size of the band when interpreting the results, which lacks intuition

[0015] To sum up: the existing common methods, such as fluorescent quantitative PCR method for gene detection, only detect a single mutation site on the mitochondria, and most fluorescent quantitative PCR methods have no internal standard and cannot be used for quality control; the operating time of microarray state chip products Long, high cost, low accuracy and requires special equipment

Large-scale screening of mitochondrial gene mutations requires fast, low-cost, and wide detection range, which cannot be met by the above products at the same time

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