Detection primer set for reverse transcription loop-mediated isothermal amplification of Noroviruses, detection method and kit

Abstract

The invention discloses a detection primer set for reverse transcription loop-mediated isothermal amplification (RT-LAMP) of Noroviruses (NV), a detection method and a kit. The detection primer set comprises an upstream outer primer F3, a downstream outer primer B, an upstream inner primer FIP and a downstream inner primer BIP, all of which are shown as the SEQ ID NO. 1, 2, 3 and 4 respectively. The RT-LAMP technology for detecting NV is simple, specific, sensitive and suitable for field detection and simple laboratory detection, and a novel virus detection method is provided. During sample detection, the sensitivity of the RT-LAMP technology reaches 97.7% higher than 90.7% of RT-PCR, and the RT-LAMP technology is more suitable for rapid detection of field samples and rapid detection in a simple laboratory and can be widely applied to hospitals, the food industry, entry and exit inspection and quarantine, quality supervision and other sectors and fields.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a norovirus reverse transcription loop-mediated isothermal amplification detection primer set, detection method and kit. Background technique: [0002] Norovirus infectious diarrhea has the characteristics of acute onset, rapid transmission, and wide coverage, and is the main cause of non-bacterial diarrhea outbreaks. Norovirus is highly infectious and mainly spreads through the intestinal tract. It can be transmitted through contaminated water, food, objects, and air. It often causes mass outbreaks in communities, schools, restaurants, hospitals, nurseries, orphanages, and the military. Noroviruses that are prevalent in different regions and at different times have relatively conserved sequences in the RNA polymerase region of their genes. According to the similarity of the nucleotide sequence of the RNA polymerase region, noroviruses are divided into five genogroups. ...

AI Technical Summary

Problems solved by technology

[0004] At present, the detection methods of norovirus pathogens include electron microscope technology, virus isolation and culture technology, immunology technology and molecular biology technology, but the first three technologies mentioned above often have low sensitivity, cumbersome and time-consuming operations, and require large and expensive instruments and equipment And other shortcomings, often have its limitations in application, especially not suitable for large-scale pathogen screening and detection and other practical applications

Molecular biology technology, especially RT-PCR detection technology, has shown its superiority in virus detection and has been applied more and more due to its fast, sensitive, and suitable for processing large-throughput samples. From the detection to the interpretation of the results, a variety of special instruments are still required, the operation is relatively cumbersome, the time required is still more than 5 hours, and there are often defects such as non-specific appearance.

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