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Immune cell culture medium system

An immune cell and culture medium technology, applied in cell culture active agent, blood/immune system cells, culture process, etc., can solve the problem of poor expansion multiple and tumor killing effect, complicated culture system, and natural killer cell culture system. improvement, etc.

Active Publication Date: 2018-07-06
ACADEMY OF MILITARY MEDICAL SCI +1
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, NK cell therapy products currently on the market have problems such as complicated culture systems, poor amplification multiples, and poor tumor killing effects, and some culture systems have safety risks.
[0004] Therefore, the culture system of natural killer cells needs to be improved

Method used

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Embodiment 1

[0050] Using the immune cell culture medium system of the embodiment of the present invention, the isolated mononuclear cells are induced and expanded into immune cells, and the activity of the immune cells is detected.

[0051] 1. Experimental method

[0052] 1. Prepare anti-human CD16 coated T75 vials

[0053] 1.1 Add 5 mL of 2.5 μg / mL anti-human CD16 monoclonal antibody dissolved in medical normal saline into a sterile culture bottle, shake the culture bottle gently to make the antibody cover the culture surface, and overnight at 4°C in the dark.

[0054] 1.2 Recover the antibody coating solution before use, wash the culture bottle once with 5 mL of normal saline, and then use 5 mL of T cell expansion medium (OpTmizer TM CTS TM T-cell expansion SFM) and washed once.

[0055] 2. Collect peripheral blood, separate peripheral blood plasma and mononuclear cells

[0056] 2.1 Collect about 100ml of human peripheral blood with a sterile blood collection bag added with antico...

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Abstract

The invention discloses an immune cell culture medium system and applications thereof. The immune cell culture medium system comprises: an immune cell activation culture medium, wherein the immune cell activation culture medium is a serum-free lymphocyte culture medium added with plasma, interleukin-2 and sapylin; an immune cell expansion culture medium, wherein the immune cell expansion culture medium is a serum-free lymphocyte culture medium added with interleukin-2 and sapylin; and an immune cell large-scale expansion culture medium, wherein the immune cell large-scale expansion culture medium is a serum-free lymphocyte culture medium added with interleukin-2. According to the present invention, with the application of the immune cell culture medium system to carry out induction expansion culture on mononuclear cells, the advantages of high induction efficiency, rapid amplification, high safety, low cost, wide source, no requirement of feeder layer cells and the like can be achievedso as to meet the requirement on a large number of nature killer cells in clinical treatment.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an immune cell culture medium system. Background technique [0002] In recent years, biological therapy has become the fifth largest treatment mode after surgery, radiotherapy and chemotherapy, and endocrine therapy, and has gradually received attention. Adoptive cellular immunotherapy (ACI) is one of the methods of cell biological therapy. It refers to the infusion of immune cells with anti-tumor activity to tumor patients, directly killing tumor cells or stimulating the body's immune response to kill tumor cells, so as to achieve the goal of treating tumors. Purpose. Currently, adoptive immune cells in clinical use include DC-CIK cells, TIL cells, LAK cells and NK cells, among which CIK cells, LAK cells and A-NK cells are all anti-cancer systems with natural killer cells as the main body. [0003] Natural killer cells (natural killer cells) are also called NK cells, mainly derive...

Claims

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Application Information

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IPC IPC(8): C12N5/0783
CPCC12N5/0646C12N2500/72C12N2500/90C12N2501/2302
Inventor 裴雪涛习佳飞岳文陈琳姚海雷南雪张亚
Owner ACADEMY OF MILITARY MEDICAL SCI
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