7 results about "Feeder Layer Cells" patented technology

The invention discloses construction of a Guangdong small-ear spotted pig spermatogonial stem cell in-vitro culture system and screening of surface markers, and belongs to the technical field of cell culture.The technical scheme includes that testis are broken firstly, then enzyme digestion, density gradient centrifugal purification and differential adherent purification are conducted, and purified spermatogonial stem cells are obtained; inoculating the obtained spermatogonial stem cells to feeder layer cells and culturing in a culture medium; the culture medium is a DMEM culture medium added with FBS and growth factors; the mass fraction of the FBS in the DMEM culture medium is 8-12%, an enzyme digestion method, a density gradient centrifugation method and a differential adhesion method are combined for application, the Guangdong small-ear spotted pig spermatogonia stem cells with certain purity are obtained, cells enriched and purified in vitro can proliferate massively, and an in-vitro culture system of the Guangdong small-ear spotted pig spermatogonia stem cells is established.

The application discloses a method for inducing NK cells based on a porous material. The method for inducing NK cells provided by the application is based on a device containing a mesh culture tank and porous gelatin sponge and a culture medium, and can efficiently induce iPSCs-NK. The method solves the problems that current differentiation culture technologies of iPSC-NK rely on feeder layer cells of animal origin, the differentiation efficiency is low, and the purity is insufficient and needs to be further sorted. The method has the potential for large-scale industrial production.

The present disclosure relates to methods and compositions for reprogramming cells to a pluripotent state. In particular, it relates to an integration- and feeder cell-free method for reprogramming primary human fibroblast cells to induced pluripotent stem cells (iPSCs).

This invention relates to a modified feeder cell and a method for culturing natural killer (NK) cells, aiming to enhance the proliferation capacity and killing activity of NK cells by using a specific feeder cell.

Provided are an HLA-E modified feeder cell and a method for culturing natural killer cells (NK cells). By means of culturing NK cells using the HLA-E-modified feeder cell, NK cells having a low expression of inhibitory receptor NKG2A and a high expression of activating receptors, such as NKG2C and NKp44, can be obtained, thereby significantly enhancing the killing activity of NK cells against target cells, particularly those with a high HLA-E expression.

The invention provides a line establishment method of primordial germ cells from chick embryo blood. The line establishment method comprises the following steps: obtaining fertilized eggs incubated to a 14-17HH period; sucking chick embryo blood from the dorsal aorta by adopting a micro-injection needle with a specific size and specific suction pressure; carrying out centrifugal treatment, and resuspending the cells by adopting a PGCs complete culture solution added with an antifungal drug; carrying out subculture by adopting a PGCs complete culture solution until the PGCs monoclonal cell line is successfully established; according to the line establishment method, a traditional enzyme digestion separation mode is not adopted, dependence on feeder layer cells is avoided, and the undifferentiated state of the PGCs can be maintained directly through culture medium components of a specific formula by adopting the chick embryo blood obtained through specific operation; compared with the prior art, the operation is more efficient and simpler; more importantly, the technical scheme can be used for quickly and successfully establishing the line, is good in repeatability and high in line establishment success rate, and can be popularized and applied to in-vitro line establishment of the PGCs and subsequent biological design breeding and model animal production.