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3 results about "Feeder Layer Cells" patented technology
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Feeder layer. In order to culture some cells, particularly at low or clonal density, it is necessary to use a layer of less fastidious cells to condition the medium. Often the cells of the feeder layer are irradiated or otherwise treated so that they will not proliferate.
Provided are an HLA-E modified feeder cell and a method for culturing natural killer cells (NK cells). By means of culturing NK cells using the HLA-E-modified feeder cell, NK cells having a low expression of inhibitory receptor NKG2A and a high expression of activating receptors, such as NKG2C and NKp44, can be obtained, thereby significantly enhancing the killing activity of NK cells against target cells, particularly those with a high HLA-E expression.