Feeder-free and serum-free human induced pluripotent stem cell isolation culture method

Abstract

The invention relates to a feeder-free and serum-free human induced pluripotent stem cell isolation culture method. By the feeder-free and serum-free human induced pluripotent stem cell isolation culture method, feeder cells and serum are not needed, a culture medium has clear components, operation is simple, human iPS cells can be efficiently and stably isolated, self-renewal and pluripotency are maintained, and a good experimental foundation is laid for final establishment of a clinical application-level human iPS cell isolation culture system.

Description

technical field

[0001] The invention relates to the field of cell engineering, in particular to a feeding layer-free and serum-free method for separating and culturing human induced pluripotent stem cells. Background technique

[0002] Nowadays, in the commonly used induced pluripotent stem cells (iPScells) isolation and culture system, it largely depends on feeder cells and serum, which are responsible for the self-renewal and pluripotency of iPS cells. plays an important role (Zhou et al., 2015).

[0003] The application of feeder cells in pluripotent cell culture has a long history. As early as in vitro culture of teratoma cells (embryonic carcinoma cells, ECC), the use of mouse embryonic fibroblasts (MEFs) that lost mitotic ability ) as feeder cells, and has been used in mouse and human embryonic stem cells (Zwaka and Thomson, 2005). Currently commonly used mouse feeder cells include two types: MEFs and STO cells. Feeder cells may play two roles in maintaining the sel...

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