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Method for directionally inducing and differentiating induced pluripotent stem cells into vascular endothelium-like cells

A technology for directional induction of pluripotent stem cells, applied in the field of directional induction and differentiation of induced pluripotent stem cells into vascular endothelial-like cells, which can solve the problems of differences in induction differentiation efficiency, cross-contamination, and insignificant long-term effects, and achieve high efficiency, The effect of short induction period and stable performance

Active Publication Date: 2015-10-28
GUANGXIU-GAOXIN LIFE SCI CO LTD HUNAN
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Problems solved by technology

However, there are still defects in the use of these cells in replacement therapy, such as the low differentiation efficiency of mononuclear cells and mesenchymal stem cells, and the insignificant long-term effect of transplantation. The differentiation ability of blood-derived EPCs is limited, and the number of starting cells is limited. It is difficult to meet the needs of treatment, etc.
[0005] Due to the different ways of inducing differentiation from hESCs or hiPSCs, there are differences in the efficiency of inducing differentiation
For example, using EBs to add endothelial-associated induction factors to induce differentiation may result in different induction and differentiation efficiencies due to the different sizes of cultured EBs; co-cultivation with stromal cells will bring cross-contamination between different species, etc.

Method used

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  • Method for directionally inducing and differentiating induced pluripotent stem cells into vascular endothelium-like cells
  • Method for directionally inducing and differentiating induced pluripotent stem cells into vascular endothelium-like cells
  • Method for directionally inducing and differentiating induced pluripotent stem cells into vascular endothelium-like cells

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Embodiment Construction

[0039] The method for directional induction of induced pluripotent stem cells to differentiate into vascular endothelial-like cells will be further described in detail mainly in conjunction with the accompanying drawings and specific examples.

[0040] Such as figure 1 As shown, a method for directional induction of induced pluripotent stem cells to differentiate into vascular endothelial-like cells in one embodiment includes the following steps:

[0041] Step S110, providing or preparing induced pluripotent stem cells.

[0042] The induced pluripotent stem cells in this embodiment mainly use human induced pluripotent stem cells, and methods such as virus infection, plasmid transfection, mRNA introduction, miRNA introduction, or chemical molecule addition can be used to reprogram fibroblasts to induce differentiation into induced pluripotent stem cells. The fibroblasts may be isolated adult, infant or fetal skin fibroblasts and the like. It can be understood that in other emb...

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Abstract

The invention relates to a method for directionally inducing and differentiating induced pluripotent stem cells into vascular endothelium-like cells. According to the method, a culture system without feeder cells is adopted, the vascular endothelium-like cells can be obtained only through expressing FLI1 related genes and activating a protein kinase C pathway, and a test shows that the purity is relatively high. Through the adoption of the method for directionally inducing and differentiating induced pluripotent stem cells into vascular endothelium-like cells, the induction period is short, the efficiency is high, the obtained vascular endothelium-like cells are stable in performance, and the problem of matrix cell pollution is avoided.

Description

technical field [0001] The invention relates to the field of cell engineering, in particular to a method for directional induction and differentiation of induced pluripotent stem cells into vascular endothelial cells. Background technique [0002] The occurrence and development of blood vessels are of great significance to the development and differentiation of various organs during embryogenesis, and to the wound repair and reproductive functions of adults. Vascular endothelial-like cells are the morphological basis for the formation of the closed cardiovascular system. A variety of cells in vitro can be induced to differentiate into endothelial progenitor cells (EPCs) / endothelial cells (endothelial cells, ECs), which can be induced by bone marrow-derived mononuclear cells, blood-derived progenitor cells, and mesenchymal stem cells Differentiated endothelial progenitor cells have shown broad therapeutic prospects. However, there are still defects in the use of these cells...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/867
Inventor 胡亮林戈卢光琇赵艳李自立
Owner GUANGXIU-GAOXIN LIFE SCI CO LTD HUNAN
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