Method for directionally inducing and differentiating induced pluripotent stem cells into vascular endothelium-like cells
A technology for directional induction of pluripotent stem cells, applied in the field of directional induction and differentiation of induced pluripotent stem cells into vascular endothelial-like cells, which can solve the problems of differences in induction differentiation efficiency, cross-contamination, and insignificant long-term effects, and achieve high efficiency, The effect of short induction period and stable performance
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[0039] The method for directional induction of induced pluripotent stem cells to differentiate into vascular endothelial-like cells will be further described in detail mainly in conjunction with the accompanying drawings and specific examples.
[0040] Such as figure 1 As shown, a method for directional induction of induced pluripotent stem cells to differentiate into vascular endothelial-like cells in one embodiment includes the following steps:
[0041] Step S110, providing or preparing induced pluripotent stem cells.
[0042] The induced pluripotent stem cells in this embodiment mainly use human induced pluripotent stem cells, and methods such as virus infection, plasmid transfection, mRNA introduction, miRNA introduction, or chemical molecule addition can be used to reprogram fibroblasts to induce differentiation into induced pluripotent stem cells. The fibroblasts may be isolated adult, infant or fetal skin fibroblasts and the like. It can be understood that in other emb...
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