Method for preparing breast cancer bone metastasis organs

A technology for bone metastasis and breast cancer, applied in the field of biomedicine, can solve the problems of low success rate

Inactive Publication Date: 2020-04-17
北京科途医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] In order to solve the problem of low success rate existing in the culture of breast cancer bone metastases organoids, the present disclosure provides a method for preparing breast cancer bone metastases organoids

Method used

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  • Method for preparing breast cancer bone metastasis organs
  • Method for preparing breast cancer bone metastasis organs

Examples

Experimental program
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Embodiment

[0049] 1. Preparation of feeder layer cells

[0050] Human bone marrow mesenchymal stem cells (ATCC, FS-0008) were plated at a density of about 500 cells per well in a 96-well plate containing 50 μl of Cosmo thermosensitive hydrogel per well, and 200 μl of breast cancer bone was added to each well. Transfer the special medium, and after culturing for 24 hours, place the entire 96-well plate covered with human bone marrow mesenchymal stem cells together with the culture solution in an X-ray irradiator for irradiation. The radiation dose is 80KV, and the irradiation time is After 30 minutes of irradiation, the whole 96-well plate plated with human bone marrow mesenchymal stem cells was stored in a constant temperature incubator at 37°C and used as a feeder layer material for later use.

[0051] 2. Preservation of breast cancer bone metastases

[0052] The fresh breast cancer bone metastases surgery tissue and / or puncture tissue are stored in a special medium for breast cancer b...

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Abstract

The invention relates to a method for preparing breast cancer bone metastasis organs, which comprises the following steps: mixing breast cancer bone metastasis cells, feeder layer cells, a temperature-sensitive hydrogel and a first culture medium, and culturing to obtain breast cancer bone metastasis organs; wherein the feeder layer cells comprise bone marrow mesenchymal stem cells without a proliferation function. By means of the technical scheme, the bone marrow mesenchymal stem cells without the proliferation function are used as feeder layer cells for breast cancer bone metastasis organ culture, and the success rate of breast cancer bone metastasis organ culture through the method is high.

Description

technical field [0001] The disclosure relates to the technical field of biomedicine, in particular to a method for preparing breast cancer bone metastases organoids. Background technique [0002] Breast cancer is a malignant tumor that occurs in the glandular epithelial tissue of the breast. 99% of breast cancer patients are women, and only 1% are men. Breast cancer cells have lost the characteristics of normal cells, and the connections between cells are loose and easy to fall off. Once breast cancer cells fall off, free breast cancer cells can spread throughout the body with blood or lymph fluid, forming metastasis, which is life-threatening. Among them, breast cancer cells Bone metastases of breast cancer are formed after the cells metastasize to adjacent bones. Bone metastasis of breast cancer accounts for 27%-50% of the first symptoms of breast cancer metastasis, and the incidence rate is 65-70% in the course of recurrent and metastatic breast cancer, which is relative...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
CPCC12N5/0693C12N2500/38C12N2501/11C12N2501/117C12N2501/119C12N2501/998C12N2502/1358
Inventor 孙志坚康平李程李堃
Owner 北京科途医学科技有限公司
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