Method for preparing breast cancer bone metastasis organs
A technology for bone metastasis and breast cancer, applied in the field of biomedicine, can solve the problems of low success rate
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[0049] 1. Preparation of feeder layer cells
[0050] Human bone marrow mesenchymal stem cells (ATCC, FS-0008) were plated at a density of about 500 cells per well in a 96-well plate containing 50 μl of Cosmo thermosensitive hydrogel per well, and 200 μl of breast cancer bone was added to each well. Transfer the special medium, and after culturing for 24 hours, place the entire 96-well plate covered with human bone marrow mesenchymal stem cells together with the culture solution in an X-ray irradiator for irradiation. The radiation dose is 80KV, and the irradiation time is After 30 minutes of irradiation, the whole 96-well plate plated with human bone marrow mesenchymal stem cells was stored in a constant temperature incubator at 37°C and used as a feeder layer material for later use.
[0051] 2. Preservation of breast cancer bone metastases
[0052] The fresh breast cancer bone metastases surgery tissue and / or puncture tissue are stored in a special medium for breast cancer b...
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