Stomach cancer tumor primary organ culture method

A culture method and organoid technology, which is applied in the field of cell biology technology and application, can solve problems such as the limitation of the effectiveness of anti-tumor drugs, and achieve the effect of low cost and easy operation

Inactive Publication Date: 2019-04-16
MIAOSHUN SHANGHAI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the primary tumor cells obtained by the above methods can well simulate the migration and invasion of tumors in vivo, and have great limitations in evaluating the effectiveness of anti-tumor drugs

Method used

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  • Stomach cancer tumor primary organ culture method
  • Stomach cancer tumor primary organ culture method
  • Stomach cancer tumor primary organ culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0018] The feeder layer cells and primary gastric cancer tumor cells were respectively obtained by enzyme digestion method and cell culture method.

[0019] The method for obtaining feeder layer cells specifically comprises the following steps:

[0020] Weigh and record (4.3g) the pericancerous tissue isolated from solid tumor patients after surgery, immerse it in a 50ml centrifuge tube filled with 20ml Hank's balanced salt solution, transfer it to the laboratory in the shortest possible time, and perform strict aseptic operation ;

[0021] Spray 70% alcohol on the surface of the 50ml centrifuge tube containing the pericancerous tissue, move the 50ml centrifuge tube into a biological safety cabinet, remove excess alcohol, open the cap of the centrifuge tube, and use sterile blunt forceps to move the pericancerous tissue into the In a 100mm petri dish, remove blood clots, rinse the pericancerous tissue three times with Hank's balanced salt solution, and keep the pericancerous ...

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Abstract

The invention relates to the field of cell biology technologies and application, and particularly relates to a stomach cancer tumor primary organ culture method. The method comprises the steps of obtaining feeder layer cells and stomach cancer tumor primary cells by adopting an enzyme digestion method and cell culture method; adding magnetic suspension particles to the feeder layer cells and the stomach cancer tumor primary cells respectively, and obtaining the magnetic particle containing feeder layer cells and stomach cancer tumor primary cells by incubation-digestion; inoculating the magnetic particle containing feeder layer cells and stomach cancer tumor primary cells to a stomach cancer tumor primary organ culture medium, executing culture under the magnetic driving conditions to obtain stomach cancer tumor primary organs. By adopting a magnetic suspension culture system, the feeder layer cells and stomach cancer tumor primary cells are co-cultured, the mutual effect of tumor microenvironment and the cells is better simulated, and the new in-vitro efficacy testing method is provided for the drug research and development industry.

Description

technical field [0001] The invention relates to the field of cell biology technology and application, in particular to a method for culturing primary gastric cancer tumor organoids. Background technique [0002] Gastric cancer is a common malignant tumor in the world, with a relatively poor prognosis and a serious threat to human health. In the current process of drug research, in vitro drug efficacy testing is generally considered to be an important test method for evaluating drug effectiveness. [0003] In the prior art, two-dimensional tumor primary culture methods are mainly used for in vitro drug efficacy testing and other drug development processes. For example, the tumor cell suspension is obtained first, and then the RPMI1640 medium and basic fiibroblast growth factor (basic fiibroblast growth factor) are used to obtain the drug development process. , bFGF), 20ng / mL epidermal growth factor (epithelial growth factor, EGF), 5μg / mL insulin (insulin) and 0.4g / 100mL bovi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
CPCC12N5/0693C12N2501/11C12N2501/115C12N2501/345C12N2501/415C12N2501/998C12N2502/00
Inventor 龚延浩周斌陈维维
Owner MIAOSHUN SHANGHAI BIOTECH CO LTD
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