The invention discloses a method for resisting human
mesenchymal stem cell aging and enhancing stem characteristics of human mesenchymal stem cells. According to the method, immune cells in human
peripheral blood and aged human mesenchymal stem cells are cultured in a
cell-to-
cell contact mode, the aging characteristics of the aged human mesenchymal stem cells can be remarkably reversed, the expression of
cell aging markers such as
beta galactosidase, P21 and P16 proteins is reduced, and meanwhile, the
cell cycle of the aged human mesenchymal stem cells can be obviously adjusted, specifically,the number of cells in the G1 stage is reduced, and the number of cells in the S stage is increased. More importantly, the method can remarkably enhance the stem characteristics of the aged human mesenchymal stem cells, such as self-renewal capacity, multiplication capacity and multi-directional differentiation potential, and the cells obtained by the culture mode are used for treating
disease models and are safe and effective. The method can be directly applied to long-term in-vitro amplification of the human mesenchymal stem cells, the problem of
cell aging in the amplification process is solved, the
cell activity is recovered, and the clinical application effect is improved.