Cell co-culture model, cell model construction method, computer equipment and storage medium

Abstract

The invention relates to a cell co-culture model, a cell model construction method, computer equipment and a storage medium, and the cell co-culture model construction method comprises the following steps: collecting cell images in a cell co-culture process under preset cell inoculation density and cell inoculation proportion in real time, extracting cell characteristic parameters of each cell in the cell image under different time nodes, and marking the category and the number of the cells in the cell image; the co-culture cell inoculation density, the cell inoculation proportion, the culture cell category and the cell co-culture time node are used as inputs, and the cell characteristic parameters of each cell are used as outputs to train a neural network, so that a cell co-culture model is obtained, and the cell culture process can be observed in real time; according to the method, the cell characteristic parameters in the cell co-culture process can be rapidly, effectively and repeatedly obtained, and the method is very important for studying the cell function state, especially studying the interaction between cells when co-culturing different types of cells.

Description

technical field [0001] The present application relates to the field of deep learning technology, and in particular to a cell co-culture model, a cell model construction method, computer equipment and storage media. Background technique [0002] Cell co-culture in clinical biological experiments has always been a difficult problem with many limitations. Taking the co-culture of mesenchymal stem cells and other cells as an example, mesenchymal stem cells are a kind of pluripotent stem cells, which have all the common characteristics of stem cells, namely self-renewal and multi-lineage differentiation capabilities. It is also the most clinically used, and the source of the most is bone marrow mesenchymal stem cells BMSC, also known as bone marrow stromal fibroblasts in the past, is a type of adult stem cells derived from mesoderm, with self-renewal and multi-directional differentiation potential, and can be differentiated It is a variety of interstitial tissues, such as bone, ...

AI Technical Summary

Problems solved by technology

Its advantage is that it highlights the effect of conditional cells on target cells, and it is easier to separate the two types of cells. However, this method does not strictly culture at the same time, and generally only the co-culture of two types of cells can be carried out, and more kinds of cells cannot be carried out. Cell co-culture, because there is no contact between the two cells, it does not show that the stromal cells have a significant support function for the parenchymal cells

[0005] At present, a variety of multi-cell co-culture models have been proposed. In the prior art, a six-hole cell co-culture culture plate including several interconnected culture holes, pistons, and polycarbonate diaphragms has been proposed, including several interconnected cells. Connected culture wells, there are through holes between adjacent culture wells, the through holes can be sealed by pistons, and the pistons are equipped with handles for easy loading and unloading. According to experimental requirements, one or more pistons can be removed during use to make the culture wells Intercommunication between them to achieve intercommunication of culture medium. The disadvantage is that they are disposable. After mixing, the cells cannot be separated uniformly.

At present, the methods for long-term continuous observation of cells mainly include visual inspection under a microscope, which has the disadvantage that it takes a long time and can only observe the state at a specific time point; through indirect observation of metabolites, a specific chemical substance MTT is added to the cells, Obtain cell status indirectly by detecting MTT, the disadvantage is that it will interfere with the normal growth of cells, and it is not sensitive to real-time detection during the amplification process; and the use of real-time sensor observation, including resistance sensors and thermal sensors, has the disadvantage that cells may be damaged, and the error is relatively large. Large, susceptible to cellular electrochemical processes

However, the state observation of cells is often transient, and the best observation time is often missed during the experiment

[0007] In addition, in the actual multi-cell culture process, it is necessary to observe or obtain the growth status and morphological rules of the cells in the culture dish by moving the culture dish. However, frequently changing the state of the culture dish may affect the survival and development of the cells in the culture dish.

In addition, at present, the main way to distinguish different cells is to use morphological detection, or to use double-labeled immunohistochemical technology or in situ hybridization technology, or to use different fluorescent labels. The above methods all involve direct manipulation of cells and are possible It will affect the natural growth of cells, which is not conducive to observing the actual growth of cells

[0008] Existing technologies have done a lot of research on rapid observation during cell culture, but it is still unable to systematically and comprehensively provide real-time characteristic parameters of different cells in the process of cell co-culture, nor can it realize the prediction and evaluation of the growth state of cell co-culture

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