76results about How to "Effective proliferation" patented technology

The invention intends to provide a cell culture apparatus which is able to realize an adequate culture according to the culture state of cells while alleviating the labor of an operator. The apparatus includes: a culture bag (18) for causing the cells to proliferate; a cell inoculation cassette (19) (or culture bag (242) as an antibody stimulating and proliferation culture vessel) for stimulating the cells by an inducer for the proliferation; a culture medium cassette (20) for storing a culture medium supplied to the culture bag (18) and the cell inoculation cassette (19); a CCD camera (88) for acquiring images of the cells in the cell inoculation cassette; and an image processing computer and an operation control computer for determining the culture state (proliferation capability and proliferation ability of the cells) of the cells from the images of the cells acquired by the CCD camera, and causing a culture operation to be carried out on the basis of the determination.

The invention discloses a composite prebiotics which is composed of fructooligosaccharid and inulin of which the mixture ratio is 1:0.1 to 10 or oligomeric galactose, the fructooligosaccharid and the inulin all of which the mixture ratio is 0.1 to 10:1:0.1 to 10. The composite prebiotics overcomes the shortcoming that the single component of the prebiotics cannot fully exert the physiological efficacy, solves the technical problem that bifidobacterium and lactobacillus are proliferated rapidly and efficiently and the efficacy of the Prebiotics perforates through each end of the colon, particularly, the conventional liquid prebiotics is made into powder, so that the quality of products is more stable and the use is more convenient. The composite prebiotics is a high-quality composite prebiotics which can completely improve the component of probiotics in the intestines and fast, continuously and effectively proliferate the probiotics.

The invention relates to the technical field of microbiological screening and application and specifically provides a novel lactobacillus fermenti strain. The lactobacillus fermenti strain has a collection number of CCTCC NO: M2016430. The strain has obvious inhibitory effects on Escherichia coli, salmonella and staphylococcus aureus, particularly has the strongest inhibitory effect on helicobacter pylori, and the diameter of an inhibition zone reaches 23mm. The lactobacillus fermenti is high in tolerance to gastric acid and bile salts, and is capable of realizing effective multiplication in a gastric acid environment. The lactobacillus fermenti has a cholesterol removal rate of 93.2%, and can maintain the survival rate of 98.5% in 1.2mmol / L H2O2; the fermented supernatant fluid and cell-free extract of the lactobacillus fermenti respectively have the hydroxyl radical scavenging rate of 95.1% and 90.4%. The lactobacillus fermenti can be widely applied to preparation of the lactic acid bacterium drink, is capable of obviously increasing the quantity of intestinal available bacteria, improving the gastrointestinal tract health and improving the immunity of the organism, and has a wide application prospect.

The invention discloses a nerve scaffold for nerve injury repairing and a preparing method and application thereof. The nerve scaffold comprises a collagen scaffold body, neural stem cells cultured on the collagen scaffold body and growth factors fixed to the collagen scaffold body through heparin. The growth factors have heparin binding domains. The preparing method includes the steps that a collagen solution is subjected to freeze drying before crosslinking, a collagen sponge obtained after freeze drying is soaked into a crosslinking solution to be crosslinked, the growth factors are fixed to the collagen scaffold body through the heparin, and the activity of the neural stem cells is maintained or propagation of the neural stem cells is promoted. According to the preparing method, materials are convenient to obtain, the steps are simple, complex steps including acid-base neutralization, dialysis and the like are avoided, the opportunity that collagen materials are in contact with harmful reagents is reduced, the safety of the obtained nerve scaffold is improved, the nerve scaffold can be molded to be in different shapes as required and can be used for filling nervous tissue losses and bridged nerve broken ends and guiding growth of nerves, nerve injury repairing and function recovery are promoted accordingly, and good stability is achieved.

The present invention provides an expression system, the system comprising: a first nucleic acid molecule having a cell specific promoter; a second nucleic acid molecule encoding a transcriptional activator; a third nucleic acid molecule having a first recognition sequence of the transcriptional activator; a fourth nucleic acid molecule having a first promoter and a first regulatory element; a fifth nucleic acid molecule encoding a first regulatory protein; a sixth nucleic acid molecule having a second recognition sequence of the transcriptional activator; a seventh nucleic acid molecule having a second promoter and a second regulatory element; an eighth nucleic acid molecule encoding a second regulatory protein; and: a ninth nucleic acid molecule configured to conditionally inhibit expression of the first regulatory protein; and a tenth nucleic acid molecule configured to conditionally inhibit expression of the second regulatory protein, wherein the first regulatory element is adaptedto inhibit the function of the first promoter by binding to the second regulatory protein, and the second regulatory element is adapted to inhibit the function of the second promoter by binding to the first regulatory protein.

Disclosed is a Madin-Darby canine kidney (MDCK)-derived cell line. The MDCK-derived cell line is derived from MDCK cells deposited under accession number ATCC CCL-34. The MDCK-derived cell line can be prepared by serum-free culture and suspension culture. Preferably, the MDCK-derived cell line has low or no tumorigenicity. The MDCK-derived cell line is preferably selected from MDCK Sky1023, MDCK Sky10234 and MDCK Sky3851. Further disclosed are a culture method for growing the MDCK-derived cells and a method for producing a vaccine virus using the MDCK-derived cells.

The present invention provides a method for producing hollow porous microspheres, comprising: a polymer solution production step which comprises dissolving a substance for inducing pore formation anda hydrophobic biodegradable polymer in a volatile solvent; a step for forming an oil-in-water (O / W) emulsion by dispersing the resulting polymer solution in an aqueous solution comprising water or a phase stabilizer; a hollow porous microsphere production step which comprises volatilizing the volatile solvent in the aqueous solution having the polymer solution dispersed therein so as to solidify the hydrophobic biodegradable polymer and causing voluntary phase separation between the hydrophobic biodegradable polymer and the substance for inducing pore formation so as to change an oil-phase polymer solution comprised in the oil-in-water (O / W) emulsion to oil-in-solid (O / S) microspheres; and a step for removing the substance for inducing pore formation comprised in the hollow porous microspheres.

A sour milk rich in L-lactic acid is prepared from the defatted or fresh milk through inoculating cheese lactobacillus, adding tomato juice and malt juice, fermenting, inoculating acidophilic lactobacillus and thermophilic streptococcus, fermenting and coagulating. It features high contents of living bacteria.

Disclosed is a Madin-Darby canine kidney (MDCK)-derived cell line. The MDCK-derived cell line is derived from MDCK cells deposited under accession number ATCC CCL-34. The MDCK-derived cell line can be prepared by serum-free culture and suspension culture. Preferably, the MDCK-derived cell line has low or no tumorigenicity. The MDCK-derived cell line is preferably selected from MDCK Sky1023, MDCK Sky10234 and MDCK Sky3851. Further disclosed are a culture method for growing the MDCK-derived cells and a method for producing a vaccine virus using the MDCK-derived cells.

The invention provides a proteolytic targeting chimeric body, a prodrug molecule for improving oral bioavailability of the proteolytic targeting chimeric body and application thereof. According to thetechnical scheme, a novel PROTAC degradation agent compound is developed on the basis of a ribociclib derivative and a CRBN ligand. Small molecules can effectively degrade CDK2 / 4 / 6 and compounds thereof in malignant melanoma at the same time; the cell cycle can be quickly reset, and apoptosis of various cancer cells, especially melanoma cells, can be induced. A mechanism should be explained as that CDK 2 / 4 / 6 deficiency may lead to synthetic lethal effects of malignant melanoma in the presence of a compound. The results show that the combination of CDK2 / 4 / 6 is expected to become a kinase target for treating solid tumors. In addition, the invention also develops a prodrug with high oral bioavailability for the first time, and is convenient for oral administration in animal tests. A universal solution is provided for oral administration of PROTAC molecules with CRBN ligands.

The invention provides pig feed preventing and treating gastrointestinal diseases. The pig feed is prepared from the following raw materials in parts by weight: 30 to 40 parts of soybean meal, 25 to 30 parts of corn, 25 to 30 parts of rice bran, 20 to 25 parts of wheat bran, 8 to 15 parts of fish meal, 3 to 7 parts of composite amino acid powder, 2 to 7 parts of oligosaccharide, 2 to 6 parts of eucalyptus globulus oil, 4 to 6 parts of traditional Chinese medicine additives, and 1 to 3 parts of table salt. The prepared feed has excellent efficacy for promoting the digestive absorption and resisting bacteria and viruses, is good in palatability, can greatly improve the feeding of pigs, and remarkably decreases the infection rate of the gastrointestinal diseases and the material-to-meat ratio; and moreover, by adopting the traditional Chinese medicine additives, the pig feed has no toxic and side effects, the immunity of the pigs can be fundamentally improved, and the safety and environmental-friendliness of meat products can be improved.

The present invention relates to a medium used for culturing mammalian cells in vitro and additives for constituting the medium, which can effectively make mammalian somatic cells without using serum when culturing mammalian somatic cells proliferation. By mixing velvet antler active polysaccharides in the medium, mammalian somatic cells can be efficiently proliferated even when the medium is completely free of serum.

The invention discloses a high-purity konjac mannan polysaccharide. The high-purity konjac mannan polysaccharide is a polysaccharide system with a weight-average molecular weight of 6000-8000Da prepared by producing konjac powder by virtue of beta-mannanase controllable hydrolysis and purifying. The polysaccharide system has physiological functions of proliferating vaginal beneficial bacteria andinhibiting harmful bacteria and can be applied to antibacterial treatment of colpitis.

The invention relates to a zostera marina seed multiplication method and belongs to the technical field of ocean resource recovery. The method particularly includes the following steps: (1), collecting zostera marine seeds, storing the seeds into fresh seawater 32psu after surface disinfection with alcohol of 70%, performing inflation saving at 17-20 DEG C, and saving in a refrigerator for 30 days at 4 DEG C before seeding; (2), starting to seed in the middle of November; (3), transplanting to a sea area in June next year, wherein the seeding method particularly includes 1, selecting a shrimp pond with water depth of 40-100cm and bottom sediment to be a sand-mud bottom; 2, making a transplanting frame; 3, seeding; 4, cultivating in the shrimp pond. During sea area transplanting, an area with water depth of subtidal zone to be 20cm, bottom sediment to be a sand-mud bottom and sand-mud proportion to be 1:3-1:5 is selected, and tidewater needs to have certain flow speed, 6cm / s properly. By the zostera marina seed multiplication method, damage to existing resources is avoided, zostera marina multiplication can be effectively performed, and effect of restoring zostera marine resource quantity is realized.

The invention relates to a platelet lysate supported micro-sphere preparation method, and aims to solve the problem of large platelet lysate consumption or poor micro-carrier adsorption effect in existing cell culture. The preparation method includes the steps of platelet lysate preparation, micro-sphere component preparation and platelet factor and micro-capsule supported micro-sphere preparation. A prepared micro-sphere is quite strong in osteoblast carrying capacity, most of cells are fitted on the micro-sphere under the condition that the ratio of osteoblasts to the micro-sphere is 500:1,and only few individual cells are scattered in culture solution. Mesenchymal stem cells on the micro-sphere are good in growth condition, cell viability exceeds 95%, doubling time is short, positive expression rate exceeds 95% in terms of purity, and the preparation method is applied to the technical field of biology.

The invention discloses a low-salt culture medium of Lactobacillus rhamnosus comprising a basic culture medium and an optimized culture medium, wherein that basic culture medium comprises yeast peptone, yeast powder, glucose, lactose, isomaltooligosaccharide, L-Malic acid, manganese sulfate, the balance of sterile water, pH 6.50-7.00. The optimized medium consistes of yeast peptone, yeast powder,glucose, lactose, isomaltooligosaccharide, L-Malic acid, citric acid, manganese sulfate, the balance of sterile water, pH 6.50-7.00. The culture medium is suitable for the propagation of Lactobacillusrhamnosus, and the number of viable bacteria in fermentation broth could reach more than 109 CFU / ml.

The invention discloses a urinary smell removal type young rabbit feed which is characterized by comprising the following raw materials in parts by weight: 320-355 parts of high-quality corns, 120-132 parts of wheat bran, 45-75 parts of bean meal, 42-50 parts of fish meal, 18-25 parts of bone meal, 8-12 parts of calcium hydrogen phosphate, 8-11 parts of salt, 55-68 parts of grass meal and 5-8 parts of a beneficial microorganism bacterium agent. The feed can be used for effectively reducing the urinary smell of rabbits while providing scientific nutrition for young rabbits.

The invention provides a separated biological membrane as well as a preparation method and application of the separated biological membrane. The invention further provides a method and an application of the biological membrane for stimulating T lymph cell proliferation; and furthermore, the invention provides a kit containing the biological membrane.

The invention provides a new application of sheep placenta extract in treatment of skin burn and trauma. The sheep placenta extract has a treatment effect obviously better than that of common medicines, has excellent restoration effect and does not leave a scar, and newly born skin and hair are undifferentiated with the original skin and hair; main treatment effects of the sheep placenta extract comprise anti-inflammatory effect and tissue recovery effect; a main restoration mechanism is as follows: the sheep placenta extract can promote rapidly proliferation of macrophages and improve activities of the macrophages, the macrophages can secrete multiple bioactive substances and multiple enzymes, directly participates in an organism restoration process and eliminates necrotic fragments of tissues and cells and pathogens at a damaged part, and the bioactive substances and the enzymes have an important regulating effect and a good recovery effect on a skin burn and trauma healing process and is capable of effectively proliferating epidermic cells so that skin tissues can be quickly healed. Animal experiments further confirm that the sheep placenta extract has a treatment effect on a mouse burned by metal, and a related mechanism of the sheep placenta extract in treatment of the skin trauma is explained.

The invention relates to a method for propagating and protecting natural enemy insects by planting corns between vegetables in a greenhouse. According the method, a present method for planting vegetables in greenhouse is kept, the row spacing of vegetables, line spacing of the vegetables and the density of the vegetables are the same as those of a present planting method. The method comprises planting two rows of corns at an inlet of a 500 m2 greenhouse, inoculating 50 Rhopalosiphum padi or Rhopalosiphum maidis on each corn when the corns are as high as 30 cm, and inoculating 10 3rd-instar ladybird larvae on each corn after 5 days. The method provides food for natural enemies in the earlier stage and later stage of production of vegetables in the greenhouse, so the natural enemies can be propagated in position in the greenhouse in advance or collected in the later, the rate and the utilization rate of colonization of the natural enemies can be raised. The natural enemies can be continuously propagated and protected by adopting the invention, and operation is simple and the cost is low.

The invention provides application of a QX type IBV cellular adapted strain MJ to preparation of a totivirus antigen ELISA inspection kit and the kit, and belongs to the technical field of virus antibody inspection. According to the application of the QX type IBV cellular adapted strain MJ to the preparation of the totivirus antigen ELISA inspection kit, the preservation number of the QX type IBVcellular adapted strain MJ is CGMCC No. 14681, the cellular adapted strain MJ does not contain other viral antigens and is only in specific binding with an IBV positive serum, and cross reaction between the cellular adapted strain MJ and other virus positive serums is avoided, effective proliferation is conducted on a Vero cell, and the cellular adapted strain MJ is applied to effectively solve the problems that purification of IBV totivirus is difficult and non-specificity is high. The invention also provides the totivirus antigen indirect ELISA inspection reagent box constructed based on thecellular adaptive strain MJ. According to the totivirus antigen indirect ELISA inspection kit, detection sensitivity is high, specificity is strong, QX type IBV early infection can be inspected, andearly antibody level of chicken flock immune from attenuated vaccine can also be inspected.

The present invention relates to an injectable composition for skin tissue regeneration or skin tissue volume increase comprising hollow porous microspheres comprising a cavity in the center thereof and a barrier surrounding the cavity and comprising fine pores. The hollow porous microspheres comprised in the composition according to the present invention have a small particle size, thus can be administered by an injection into skin tissue, and allow moving skin tissue cells to effectively proliferate within the cavities. After a predetermined period elapses, the newly proliferated skin tissuecells maintain the volume thereof even after the hollow porous microspheres biodegrade inside skin; and thus, skin tissue regenerative effects can be safely maintained for a long time.

The invention provides an embroyogenic calli induction and proliferation method of pinus elliottii engelm. The method includes the steps of 1), induced culture of embroygenic calli, namely placing immature seed embryos of the pinus elliottii engelm in an embroyogenic calli induction medium (with a DCR culture medium as a basic culture medium added with, by weight, 30g / L of saccharose, 7g / L of carrageenan, 1.0mg / L-2.0mg / L of 2,4-dichlorophenoxyacetic acid (2,4-D), 1.0mg / L-2.5mg / L of N6-furan methyl adenine (KT), 300mg / L of L-glutamine, 250mg / L-500mg / L of casein hydrolysate, 1g / L of inose and 250mg / L of 2-(N-morpholine) ethylsulfonic acid monohydrate) for induced culture to obtain primary embryogenic calli; 2), proliferation of the embryogenic calli, namely transferring the embryogenic callli obtained in the step 1) into a proliferation culture medium (with the DCR culture medium as the basic culture medium added with, by weight, 30g / L of maltose, 7g / L of carrageenan, 0.5mg / L-1.0mg / L of2,4-D, 0.5mg / L-1.0mg / L of 6-benzylamino adenine (6-BA), 300mg / L of L-glutamine and 250mg / L-500mg / L of casein hydrolysate for proliferation culture to obtain proliferating embryogenic calli.

The invention discloses stirred yoghurt containing fibroin and a preparation method thereof, belonging to the technical field of milk products. The stirred yoghurt containing fibroin is characterized by comprising the following compositions by weight in 1000g raw material mixture: 50 to 90 grams of white granulated sugar, 3 to 6 grams of hyper-viscosity yoghurt stabilizing agent, 20 to 120 grams of fibroin powder, 15 to 25 grams of skimmed milk powder, 0.06 to 0.1 gram of yoghurt essence, 0.04 to 0.1U of directed-vat-set freeze-drying yoghurt starter, and the balance fresh milk. The stirred yoghurt containing fibroin prepared by the method has simple preparation method, and the produced product enjoys white and moist, fine and smooth mouth feel, a sour-and-sweet taste, and has more protein and viable lactobacillus than common yoghurt.

The invention discloses a multifunctional cabin combined artificial fish reef. The multifunctional cabin combined artificial fish reef comprises a lower quadrangular frustum pyramid main body, a middle support column and an upper umbrella-shaped structure, wherein each slope of the lower quadrangular frustum pyramid main body is provided with openings, the interior of the lower quadrangular frustum pyramid main body is divided into cabins by transverse and vertical partition plates, the cabins communicate through the openings, a part of cabins are a darkroom structure which not directly communicate with the outside, the umbrella-shaped structure is located above the quadrangular frustum pyramid main body and is fixedly connected with the quadrangular frustum pyramid main body through a connecting bolt on the middle supporting column, the umbrella-shaped structure is provided with an algae attachment base and an opening, the upper surface and the inclined surfaces of the quadrangular frustum pyramid main body are rough surfaces, and the upper surface of the top umbrella-shaped structure is a porous surface which is easy for algae attachment. The artificial fish reef has a shadow-rich structure, the multi-cabin structure can become a place for refuge, rest and spawning of various fishes, algae and invertebrate organisms are easy to attach to the porous plastid on the surface, the attachment density is high, and baits are provided for the fishes, sea cucumbers, abalones and the like.

The invention discloses a preparation method and application of a trophoblast modified by genetic engineering, and aims to research whether K562 cells can stably express IL-21, OX40L and CD48 molecules at the same time and enhance NK cell proliferation and tumor killing ability or not. K562 gene engineering cells simultaneously expressed by the three molecules are used as trophoblasts to culture NK, so that the amplification multiple, purity and killing function of the NK are improved; according to the preparation method, K562 cells can be used for simultaneously and stably expressing membrane binding interleukin-21 (mbIL-21), OX40L molecules and CD48 molecules to prepare an engineering cell strain named as K562-ZHX trophoblast cells, then the engineering cell strain is irradiated with cobalt-60 to obtain trophoblast amplified NK cells, the activity of the NK cells prepared by the method is improved by 3 times, the cell amplification can be improved by more than 300 times, a large amount of time and cost can be saved, and a practical foundation can be laid for obtaining high-purity, high-amplification-multiple and high-killing-activity NK cells.

The invention relates to a method for preserving raw milk by introducing nitrogen to the raw milk. The method concretely comprises the following steps: preparing nitrogen by using air, and carrying out purifying treatment on the prepared nitrogen; carrying out centrifuge purifying treatment on the raw milk, and pre-pasteurizing; and introducing nitrogen to discharge oxygen dissolved in the raw cow milk in order to nitrogen filled cow milk. Nitrogen used in the preservation number of the raw milk accounts for 78% of the volume of air, exists in air in a single substance form, is inexhaustible, is a colorless, nontoxic and tasteless inert gas, is highly safe, and can be well and uniformly mixed with the raw milk, and the introduction amount of nitrogen can be flexibly adjusted according to the difference of processing products. The method has the advantages of simplicity, easy operation, preservation and processing cost saving, improvement of the production efficiency of an enterprises, and low requirements on equipment, and is suitable for the large scale production popularization of the enterprise.

Belonging to the technical field of food processing, the invention discloses a preparation method of composite probiotic powder. The composite probiotic powder comprises freeze-dried fungus powder, prebiotics and sugar alcohols; the freeze-dried fungus powder is screened from healthy intestinal tracts of Chinese human bodies and traditional fermented foods, and is more suitable for the intestinaltracts of Chinese people. And the composite probiotic powder has the efficacy of relieving constipation and atopic dermatitis pruritus, regulating intestinal flora, relieving hyperlipidemia, relievingheavy metal toxicity, resisting oxidation, relieving inflammation, regulating intestinal flora, resisting oxidation, scavenging free radicals, reducing blood pressure, relieving type II diabetes mellitus and the like. According to the preparation method disclosed by the invention, the probiotic liquid is firstly concentrated, then freeze-drying is carried out to remove moisture, and then the product is uniformly mixed with granulated four prebiotics and three sugar alcohols according to a certain proportion, so that the flavor and taste of the product are greatly improved, and the product canalso be taken by diabetics orally.

The invention discloses a urea bacillus SC-7 and application thereof in compost. The preservation serial number of the provided urea bacillus SC-7 is CGMCC No.14961, and the urea bacillus can rapidlygrow and propagate under high temperature. The urea bacillus is subjected to fermenting culture to prepare liquor or a solid microbial agent containing the urea bacillus, the liquor or the microbial agent is added into the compost, in this way, the temperature-rising time of the compost is remarkably shortened, meanwhile, the high-temperature period of the compost is obviously prolonged, then decomposing of the compost is accelerated, and meanwhile degrading of residual antibiotics is promoted. The urea bacillus or the bactericide containing the urea bacillus has high removal efficiency for common antibiotics of different properties or categories, so that the residual antibiotics are effectively removed, and influences on human health and environment of antibiotic residues are reduced.