The invention provides an embroyogenic calli induction and proliferation method of pinus elliottii engelm. The method includes the steps of 1), induced culture of embroygenic calli, namely placing immature seed embryos of the pinus elliottii engelm in an embroyogenic calli induction medium (with a DCR culture medium as a basic culture medium added with, by weight, 30g/L of saccharose, 7g/L of carrageenan, 1.0mg/L-2.0mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D), 1.0mg/L-2.5mg/L of N6-furan methyl adenine (KT), 300mg/L of L-glutamine, 250mg/L-500mg/L of casein hydrolysate, 1g/L of inose and 250mg/L of 2-(N-morpholine) ethylsulfonic acid monohydrate) for induced culture to obtain primary embryogenic calli; 2), proliferation of the embryogenic calli, namely transferring the embryogenic callli obtained in the step 1) into a proliferation culture medium (with the DCR culture medium as the basic culture medium added with, by weight, 30g/L of maltose, 7g/L of carrageenan, 0.5mg/L-1.0mg/L of2,4-D, 0.5mg/L-1.0mg/L of 6-benzylamino adenine (6-BA), 300mg/L of L-glutamine and 250mg/L-500mg/L of casein hydrolysate for proliferation culture to obtain proliferating embryogenic calli.