34 results about "Platelet factor" patented technology

A composition and method for enhancing tissue growth, regeneration, and repair includes a Biological Glue formed by extraction of an Extremely Platelet Rich Plasma (EPRP) derived from whole blood, and subsequent activation and clotting. The Biological Glue may be utilized alone to fill defects or may be used as an adhesive agent for other biological and non-biological materials. These materials may include processed thrombus derived from the activation of EPRP. Additionally, the Extremely Platelet Rich Plasma may be impregnated with directly harvested or cultured cells, including stem cells, or other materials, prior to activation, to form a Biological Implant that may be implanted in vivo. A Platelet Factor Enriched Serum (PFS) derived from the activation of the Extremely Platelet Rich Plasma (EPRP) may be added to the cell cultures in preparation of a Biological Implant, in order to provide additional growth factors that speed the development of the cell cultures.

Methods for determining the presence of heparin/platelet factor 4 antibodies in a sample suspected to contain heparin/platelet factor 4 antibodies are provided, along with apparatus suitable for performing the methods. The method depends upon a color visualization indicating the presence or absence of heparin/platelet factor 4 antibodies in the sample. Preferred methods comprise contacting the sample with particles being complexed to platelet factor 4 (PF4) and which particle-complexed PF4 reacts specifically with heparin/platelet factor 4 antibodies, passing the sample/particle mixture through a filter, and then analyzing the color of the filtrate. The presence of heparin/platelet factor 4 antibodies in the sample is established where the color of the filtrate is substantially different from the color of the receptor-bearing particles.

The invention discloses a preparation method of an autologous platelet-factor-rich plasma (PFRP) preparation. The preparation method is characterized by comprising the steps of: A, preparation of autologous PFRP, to be specific, collecting autologous whole blood, and preparing the autologous PFRP in a centrifuging and freeze-thawing manner; and B, preparation of the PFRP preparation, to be specific, mixing the obtained PFRP with a biological scaffold/autologous somatic cells according to a volume ratio of (3:1)-(1:1) to obtain the PFRP preparation. By means of specially processing the autologous whole blood to obtain the autologous PFRP, pollution risks caused by adding an activating agent are reduced, and the immune risks of a membrane antigen during heterogenous usage are avoided. The autologous PFRP preparation prepared according to the method not only has a good effect of repairing damaged tissues such as meniscus, but also has very good anti-wrinkle, filling and lifting effects.

The invention provides a method for selectively modifying a heparin structure by using biological enzyme, which can improve anticoagulation activity of heparin, reduce combination of heparin with blood protein such as platelet factors and the like, and reduce toxic and side effects. The invention belongs to the field of biological medicine. The antithrombus and anticoagulation activities of the heparin derivatives prepared by the method are 2 to 3 times higher than common heparin medicament, the 2-O-sulfate and 6-O-sulfate contents are about 20 percent of the common heparin, and the combination capability of the heparin derivatives with the platelet factors is about 20 percent of the common heparin. The novel heparin derivatives have high anticoagulation activity and low side effect.

Atopic asthma is a chronic, inflammatory lung disease characterized by recurrent breathing problems in response to an allergen. Platelets play an important role in this allergic inflammatory process, by releasing preformed mediators like platelet factor 4 (PF4) and regulated upon activation in normal T cells expressed and secreted (RANTES) upon activation causing eosinophil chemotaxis. The present invention relates to allelic variants of the human Inositol polyphosphate 4-phosphatase (INPP4A) gene and splice variants of the coding sequence, which encodes INPP4A enzyme known to be an important regulator of platelet activation; and provides primers and methods suitable for the detection of these allelic variants for applications such as molecular diagnosis, prediction and prevention of an individual's disease susceptibility, and/or the genetic analysis of the INPP4A gene in a population. The invention also provides an association with the expression profile of INPP4A protein in the mouse model of asthma. Specifically, the invention provides a method for detection of predisposition to atopic disorders/other immunological disorders such as, autoimmune disorders, inflammatory disorders, cancer, multiple sclerosis, fibrosis, tuberculosis, sarcoidosis, hypertension and disorders developing due to hypertension, diabetes and disorders developing due to diabetes, alcohol abuse, anxiety, asthma, chronic obstructive pulmonary disease (COPD), cholecystectomy, degenerative joint disease (DJD), seizure disorder, arthritis, etc. where human Inositol polyphosphate 4-phosphatase (INPP4A) might play an important role due to its involvement in platelet action.

The invention relates to a platelet lysate supported micro-sphere preparation method, and aims to solve the problem of large platelet lysate consumption or poor micro-carrier adsorption effect in existing cell culture. The preparation method includes the steps of platelet lysate preparation, micro-sphere component preparation and platelet factor and micro-capsule supported micro-sphere preparation. A prepared micro-sphere is quite strong in osteoblast carrying capacity, most of cells are fitted on the micro-sphere under the condition that the ratio of osteoblasts to the micro-sphere is 500:1,and only few individual cells are scattered in culture solution. Mesenchymal stem cells on the micro-sphere are good in growth condition, cell viability exceeds 95%, doubling time is short, positive expression rate exceeds 95% in terms of purity, and the preparation method is applied to the technical field of biology.

The invention discloses a bottom-layer blood platelet factor patch and a preparation method of a sPL blood platelet factor gel patch membrane. The bottom-layer blood platelet factor patch and the preparation method of the sPL blood platelet factor gel patch membrane aim at solving the problems that a blood platelet plasmagel product has to be prepared in situ, and a blood platelet factor patch membrane is variable in shape and uneven in thickness and component. The bottom-layer blood platelet factor patch comprises a bottom-layer lining membrane and an upper-layer cover membrane, the middle area of the bottom-layer lining membrane is sunken downward to form a blood platelet factor bearing area, and the blood platelet factor bearing area is coated with collagen and an activating agent; blood platelet factors quickly make contact with the collagen and the activating agent and are quickly and uniformly activated. The bottom-layer blood platelet factor patch and the preparation method of the sPL blood platelet factor gel patch membrane overcome the defects that the conventional blood platelet plasmagel product cannot achieve standardization and cannot be prepared in advance, through a conventional spray coating mode or a conventional mixing mode, the gel patch membrane is not shaped and is uneven in thickness and variable in shape, and gel is crumpled and shrunken. The bottom-layer blood platelet factor patch and the preparation method of the sPL blood platelet factor gel patch membrane are applied to the field of blood platelet factor gel patch membranes.

The invention discloses a preparation method of an allograft-derived platelet-rich gelator. The method comprises the steps as follows: (1) umbilical cord blood collection; (2) primary centrifugation:centrifuging a blood sample at the centrifugation parameter of 1500 r/min for 10 min; sucking a supernatant, a middle layer and the upper end of a red blood cell layer for 0.5-1 mm after centrifugation, and performing secondary centrifugation; (3) secondary centrifugation: performing secondary centrifugation at the centrifugation parameter of 2800 r/min for 10 min; sucking a supernatant liquid after centrifugation until 5 ml of the lower layer is left, which is the platelet-rich factor; and (4) uniformly mixing the platelet-rich factor with a calcium chloride-bovine thrombin solution in the volume ratio being 1:(8-10), leaving the mixture to stand for 2 min or longer to obtain the platelet-rich gelator. The allograft-derived platelet-rich gelator prepared by the method can be used for allograft therapy, has concentration up to 9 times that of original whole blood, can be used for acute and chronic wound repair and can be used as compound artificial bone for treatment of nonunion.

The invention discloses a preparation method and application of conditioned serum rich in cytokines, and the preparation method of the conditioned serum comprises the following steps: taking fresh blood, centrifuging, taking PRP part, freezing, thawing and centrifuging to obtain platelet factor-rich plasma, adding sterile glass beads into the rest part, and carrying out constant-temperature oscillation incubation to obtain the conditioned serum rich in the cytokines. And centrifugally filtering to obtain conditioned serum rich in IL-1Ra, and mixing the platelet factor-rich plasma with the conditioned serum rich in IL-1Ra to obtain conditioned serum rich in cytokines. According to the conditioned serum rich in cytokines prepared by the method, platelet factors and IL-1Ra are fully enriched, the content is greatly increased, and meanwhile, the conditioned serum has the effects of repairing tissue damage, recovering functions and treating tissue inflammation and degeneration, and can be used for treating inflammatory arthritis and other inflammatory diseases caused by factors such as trauma, inflammation, infection and degeneration.

The invention relates to the field of cell medical beauty, and discloses a skin fibroblast precursor cell isolated culture and preparation preparing method. Fibroblast precursor cells are obtained from the autologous skin tissue through a tissue block adherence method. Accordingly, autologous platelet-rich factor plasma is further utilized for expanding the autologous skin fibroblast precursor cells, and the sufficient amount of autologous skin fibroblast precursor cells are obtained. The skin fibroblast cells have the functions of secreting collagen and hyaluronic acid and other extracellularmatrices, and the good effect is achieved for wrinkle removing, soft tissue repairing and facial rejuvenation.

The present invention relates to an activation test for diagnosing heparin-induced thrombocytopenia. More especially, the invention relates to determine functionality and an easy automation test of the heparin-induced thrombocytopenia (HIT). What is measured is the secretion of PF4 (platelet factor 4) from activated thrombocytes.

The invention relates to a neural tube defect prenatal noninvasive diagnosis molecular marker which comprises 6 miRNA (miR-144, miR-142-3p, miR-720, miR-1275, miR-575 and miR-765) and 12 proteins (pro-protein convertase subtilisin/kexin 9, ceruloplasmin, leukaemia inhibitory factor, 14-3-3, paraoxonase, plasma alpha-globulin inhibiting factor H4, serum S protein, glycosyl-phosphatidylinositol specific phospholipase D1, fetuin A, amyloid protein P, plasma alpha-globulin inhibiting factor H2 and platelet factor 4). The invention further provides application of the molecular marker.

A composition for prolonging skin aging function and improving its preparation method. It relates to a composition and its preparation method. The short-term effect is not obvious and the long-term effect is not long-lasting due to the poor effect of continuous secretion, the poor viscosity of the local simple factor injection, the weak support effect on the base tissue, and the poor ability to balance the cells and tissues. The active ingredients of the composition of the present invention include platelet factor, fibroblast, low molecular weight hyaluronic acid, laminin, superoxide dismutase, sodium gluconate, sodium acetate, sodium chloride, potassium chloride and magnesium chloride. The present invention can prolong the effect of improving skin aging function. Through the application of this combination, the effect of pure platelet factor and pure fibroblast on improving skin aging function can be prolonged.

Provided herein are isolated antibodies or antibody fragments thereof that immunospecifically bind to platelet factor 4 (PF4). In some embodiments the isolated antibodies or antigen-binding fragments thereof comprise a light chain CDR and framework region comprising SEQ ID NO: 4 and a heavy chain CDR and framework region comprising SEQ ID NO: 10. Also provided herein are methods for treating heparin-induced thrombocytopenia (HIT) and methods for reducing the likelihood that subject will become afflicted with HIT. Further disclosed are uses of the isolated antibodies or antibody fragments in the treatment of HIT or the manufacture of compositions for the treatment of HIT.

The invention relates to the technical field of wet tissues, in particular to a wet tissue with functions of hemostatic and accelerating wound healing and preparation method thereof. According to thewet tissue, plantain and rhododendron are compatible with each other, the number of blood platelet can be effectively increased, the activity of platelet factor can be enhanced, the time for generating thromboplastin is shortened, blood clotting is accelerated, and a function of fast blood stopping is achieved; a lithospermum extracting solution, hazelnut, gulfweed, passionflower, and wheat germ oil are taken as raw materials for preparation of the wet tissue, and are mutually matched with one another, a function of cell activating is achieved, human metabolism is promoted, and wound healing is accelerated; furthermore, winkles generated due to cutis laxa and aging can further be prevented, and functions of skin repairing and skin restoring are achieved; and in addition, sebum secretion can be adjusted, skin is protected, evaporation of moisture from the skin is reduced, and the good moisturizing effect is achieved.