A low-salt culture medium for Lactobacillus rhamnosus and a culture method thereof

A technology of Lactobacillus rhamnosus and culture method, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of salty taste, affecting application, high salinity of supernatant after centrifugation, etc., and achieve inorganic salt The effect of low content and stable strain

Active Publication Date: 2019-01-18
江西仁仁健康产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The commonly used medium for Lactobacillus rhamnosus (MRS) usually contains a relatively high concentration of inorganic salts, which will lead to high salinity of the bacteria, fermentation broth, and supernata

Method used

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  • A low-salt culture medium for Lactobacillus rhamnosus and a culture method thereof
  • A low-salt culture medium for Lactobacillus rhamnosus and a culture method thereof
  • A low-salt culture medium for Lactobacillus rhamnosus and a culture method thereof

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0030] Example 1 A Lactobacillus rhamnosus culture medium and culture method

[0031] (1) A Lactobacillus rhamnosus culture medium, including: a low-salt basic medium and a low-salt optimized medium;

[0032] Formula and preparation of Lactobacillus rhamnosus low-salt basic medium, formula: yeast peptone 22g / L, yeast powder 11g / L, glucose 32g / L, lactose 11g / L, isomalt-oligosaccharide 3g / L, L-apple Acid 6g / L, manganese sulfate 0.06g / L, the balance is purified water, pH 6.8; Weigh the components according to the formula ratio, mix, heat to dissolve, and sterilize at 115°C for 30 min.

[0033] Formula and preparation of Lactobacillus rhamnosus low-salt optimized medium, formula: yeast peptone 22g / L, yeast powder 11g / L, glucose 32g / L, lactose 11g / L, isomalt-oligosaccharide 3g / L, L-apple Acid 6g / L, citric acid 6g / L, manganese sulfate 0.06g / L, the balance is purified water, pH 6.8; Weigh the components according to the formula ratio, mix, heat to dissolve, and sterilize at 115°C for 30 mi...

Example Embodiment

[0046] Example 2 Single factor screening experiment of low-salt basic medium components

[0047] At present, the most used medium for the cultivation of Lactobacillus rhamnosus is the MRS medium. In order to achieve low-salt culture and large-scale growth of Lactobacillus rhamnosus, a low-salt medium suitable for the propagation of Lactobacillus rhamnosus is sought. Used in large-scale industrial production of freeze-dried Lactobacillus rhamnosus powder as a food additive, and low-salt improvement experiments on MRS medium.

[0048] Increase the nitrogen source and carbon source content in the MRS medium, reduce the content of inorganic salts, and conduct single factor experiments for the diammonium hydrogen citrate, dipotassium hydrogen phosphate, magnesium sulfate and manganese sulfate components in the MRS medium formula. The influence of each component on the number of viable bacteria of Lactobacillus rhamnosus.

[0049] Compared with the MRS medium, the low-salt basal medium of...

Example Embodiment

[0058] Example 3 Single factor screening experiment of low-salt optimized medium components

[0059] Select the bacterial liquid cultured in the basic medium of formula IV in Example 2, and inoculate the tertiary culture bacterial suspension into a 200L fermentor equipped with 120L low-salt optimized medium according to the inoculum amount of 5%, and carry out the secondary bacterial species Fermentation. Compared with the MRS medium, the low-salt optimized medium of Lactobacillus rhamnosus increases the content of nitrogen and carbon sources, reduces the types and content of inorganic salts, and removes Tween 80. The medium was prepared according to the following medium formulas, and Lactobacillus rhamnosus was cultured under the same culture conditions, the number of viable bacteria in the fermentation broth was detected, and the conductivity of the supernatant after the fermentation broth was centrifuged.

[0060] MRS medium formula: yeast peptone 10g / L, yeast powder 5g / L, beef...

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Abstract

The invention discloses a low-salt culture medium of Lactobacillus rhamnosus comprising a basic culture medium and an optimized culture medium, wherein that basic culture medium comprises yeast peptone, yeast powder, glucose, lactose, isomaltooligosaccharide, L-Malic acid, manganese sulfate, the balance of sterile water, pH 6.50-7.00. The optimized medium consistes of yeast peptone, yeast powder,glucose, lactose, isomaltooligosaccharide, L-Malic acid, citric acid, manganese sulfate, the balance of sterile water, pH 6.50-7.00. The culture medium is suitable for the propagation of Lactobacillusrhamnosus, and the number of viable bacteria in fermentation broth could reach more than 109 CFU/ml.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a low-salt culture medium and a culture method of Lactobacillus rhamnosus. Background technique [0002] Microbial culture medium (Medium) is an artificially prepared nutrient for the growth and maintenance of microorganisms. It mainly provides nutrients such as carbon sources and nitrogen sources required for the growth of microorganisms, and generally contains carbohydrates, nitrogenous substances, and inorganic salts (including trace elements) and water etc. Different media can add some growth factors according to actual needs. [0003] Studies have found that many modern chronic diseases, such as high blood pressure, kidney disease, upper respiratory tract infection, heart disease, etc., are positively correlated with excessive salt consumption. Low-salt diet has become a healthy way of life, so high salt is one of the key factors restricting the application of Lact...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/225
CPCC12N1/20
Inventor 张春宇余萍王聪耀闵祥博
Owner 江西仁仁健康产业有限公司
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