A low-salt culture medium for Lactobacillus rhamnosus and a culture method thereof
A technology of Lactobacillus rhamnosus and culture method, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of salty taste, affecting application, high salinity of supernatant after centrifugation, etc., and achieve inorganic salt The effect of low content and stable strain
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Embodiment 1
[0030] Embodiment 1 A kind of Lactobacillus rhamnosus substratum and culture method
[0031] (1) a culture medium for Lactobacillus rhamnosus, comprising: low-salt basal medium and low-salt optimization medium;
[0032] Formula and preparation of Lactobacillus rhamnosus low-salt basal medium, formula: yeast peptone 22g / L, yeast powder 11g / L, glucose 32g / L, lactose 11g / L, isomaltooligosaccharide 3g / L, L-apple Acid 6g / L, manganese sulfate 0.06g / L, the balance is purified water, pH 6.8; weigh each component according to the formula ratio, mix, heat to dissolve, and sterilize at 115°C for 30min.
[0033] Formula and preparation of Lactobacillus rhamnosus low-salt optimized medium, formula: yeast peptone 22g / L, yeast powder 11g / L, glucose 32g / L, lactose 11g / L, isomaltooligosaccharide 3g / L, L-apple Acid 6g / L, citric acid 6g / L, manganese sulfate 0.06g / L, the balance is purified water, pH 6.8; weigh each component according to the formula ratio, mix, heat to dissolve, and sterilize a...
Embodiment 2
[0046] The single factor screening experiment of embodiment 2 low-salt basal medium components
[0047] At present, the most used culture medium for Lactobacillus rhamnosus is MRS medium. In order to achieve the purpose of low-salt culture and large-scale growth of Lactobacillus rhamnosus, it is necessary to find a low-salt medium suitable for the proliferation of Lactobacillus rhamnosus. It is applied to the large-scale industrial production of Lactobacillus rhamnosus freeze-dried powder as a food additive, and the low-salt improvement experiment of MRS medium is carried out.
[0048] Increase the content of nitrogen source and carbon source in the MRS medium, reduce the content of inorganic salts, conduct single factor experiments on the components of diammonium hydrogen citrate, dipotassium hydrogen phosphate, magnesium sulfate and manganese sulfate in the formula of MRS medium to investigate Effect of each component on the viable count of cultured Lactobacillus rhamnosus. ...
Embodiment 3
[0058] The single factor screening experiment of embodiment 3 low-salt optimized culture medium components
[0059] Select the bacterium liquid cultivated in the basal medium of Formula IV in Example 2, and inoculate the tertiary culture suspension into a 200L fermenter equipped with 120L low-salt optimized medium according to 5% inoculum size, and carry out the secondary culture of the bacteria. fermentation. Compared with the MRS medium, the low-salt optimized medium of Lactobacillus rhamnosus increases the content of nitrogen source and carbon source, reduces the type and content of inorganic salt, and removes Tween 80. The culture medium was prepared according to the following medium formulas of each group, and Lactobacillus rhamnosus was cultivated under the same culture conditions, and the number of viable bacteria in the fermentation broth and the conductivity of the supernatant after centrifuging the fermentation broth were detected.
[0060] MRS medium formula: yeast...
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