New application of sheep placenta extract in treatment of skin burn and trauma
A technology of sheep placenta and extract, applied in the field of medicine, can solve the problems such as unreported effect of sheep placenta on skin burn wounds, waste of resources, environmental pollution and the like
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[0014] Preparation of sheep placenta extract: take healthy fresh sheep placenta and add 5 times the total amount of normal saline (0°C), then make a slurry with liquid nitrogen, centrifuge at 4000rpm and centrifuge for 10min to obtain the supernatant, adjust its protein concentration to 50mg / mL, is sheep placenta extract;
[0015] Experimental animals: 100 healthy adult SD rats, half male and half male, weighing 180-200g, provided by Guangzhou Experimental Animal Center;
[0016] Experimental design: Divide the animals into a treatment group and a control group. Taking metal burns as an example, observe the recovery state of sheep placenta extract on the burned surface of rats. The treatment group is sprayed with sheep placenta extract every day, and its protein concentration is 100mg / mL ;
[0017] The production of the third-degree burn model of rats: use a small piece of asbestos cardboard 8cm×5cm, hollow out the middle, with an area of 4cm×3.5cm, take 100 rats, and divi...
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