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Urea bacillus SC-7 and application thereof in compost

A Bacillus, SC-7 technology, applied in the field of environment and agricultural biology, can solve problems such as the inability to maintain activity, and achieve the effects of effective degradation, prolonged high temperature period, and improved composting efficiency

Active Publication Date: 2018-10-12
BEIJING AGRO BIOTECH RES CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, most of the strains used in composting inoculum are mesophilic strains, such as Bacillus, lactic acid bacteria, Trichoderma, etc. Although some microorganisms can withstand the high temperature of composting, they cannot maintain activity and play a role in high temperature conditions.

Method used

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  • Urea bacillus SC-7 and application thereof in compost
  • Urea bacillus SC-7 and application thereof in compost
  • Urea bacillus SC-7 and application thereof in compost

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The preparation of embodiment 1 liquid microbial agent

[0042] The preparation method of the liquid bacterial agent containing Bacillus urea SC-7 is as follows.

[0043] (1) Strain activation: under aseptic conditions, pick the Bacillus urea SC-7 strain stored in the slant medium at 4°C at low temperature, streak it on the fresh beef extract peptone solid medium, and culture it at 50°C±2°C 24 hours.

[0044] Beef extract-peptone medium: 3.0g beef extract, 10.0g peptone, 5.0g NaCl, 15-20g agar, make up to 1L with distilled water, adjust the pH to 7.4-7.6 with 1N hydrochloric acid, sterilize at 121°C for 20 minutes.

[0045] (2) Seed culture: prepare beef extract peptone liquid medium: beef extract 0.6g, peptone 2g, NaCl 1g, add water to make up to 200ml, 1N hydrochloric acid to adjust pH to 7.4-7.6, divide into two 250ml triangular flasks, each bottle 100ml, sterilized at 121°C for 20 minutes. Inoculate the Bacillus urea SC-7 activated in the step (1) into the above-...

Embodiment 2

[0047] The preparation of embodiment 2 solid bacterial agents

[0048] The preparation method of the solid microbial agent containing Bacillus urea SC-7 is as follows.

[0049] (1) Strain activation: under aseptic conditions, pick the Bacillus urea SC-7 strain stored in the slant medium at 4°C at low temperature, streak it on the fresh beef extract peptone solid medium, and culture it at 50°C±2°C 24 hours. The preparation of the beef extract peptone solid medium is the same as the step (1) of Example 1.

[0050] (2) Seed culture: prepare beef extract peptone liquid medium, beef extract 4.8g, peptone 16g, NaCl 8g, add water to make up to 1.6L, 1N hydrochloric acid to adjust pH to 7.4~7.6, divide into 500ml triangular flasks, each bottle contains liquid 160ml, 10 bottles in total, sterilized at 121°C for 20 minutes. Ten bottles of Bacillus urea SC-7 activated through step (1) were inoculated in the above-mentioned beef extract peptone liquid medium, 50° C., 200 rpm, and cultu...

Embodiment 3

[0053] Application of Example 3 Bacillus urea SC-7 bacterial agent in composting

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Abstract

The invention discloses a urea bacillus SC-7 and application thereof in compost. The preservation serial number of the provided urea bacillus SC-7 is CGMCC No.14961, and the urea bacillus can rapidlygrow and propagate under high temperature. The urea bacillus is subjected to fermenting culture to prepare liquor or a solid microbial agent containing the urea bacillus, the liquor or the microbial agent is added into the compost, in this way, the temperature-rising time of the compost is remarkably shortened, meanwhile, the high-temperature period of the compost is obviously prolonged, then decomposing of the compost is accelerated, and meanwhile degrading of residual antibiotics is promoted. The urea bacillus or the bactericide containing the urea bacillus has high removal efficiency for common antibiotics of different properties or categories, so that the residual antibiotics are effectively removed, and influences on human health and environment of antibiotic residues are reduced.

Description

technical field [0001] The invention relates to the field of environmental and agricultural biotechnology, in particular to a strain of bacillus urea SC-7 and its application in compost preparation. Background technique [0002] In order to meet the growing demand of consumers for livestock and poultry products, my country's farming model has shifted from retail farming to large-scale and intensive farming. Because antibiotics have good effects in promoting growth, preventing and treating animal diseases, they have been widely used in intensive livestock and poultry farming for a long time. According to statistics, the total consumption of antibiotics in my country in 2013 was about 162,000 tons, of which 52% were veterinary antibiotics. However, most of these antibiotics used in livestock and poultry farming cannot be completely absorbed by animals, and about 60-90% of the antibiotics are discharged into the environment in the form of original drugs or primary metabolites ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C05F17/00C12R1/01
CPCC05F11/08C12N1/20C05F17/20C12N1/205C12R2001/01Y02W30/40
Inventor 仇天雷李复煌王旭明郑瑞峰陈余高敏孙艳梅王梁常卓
Owner BEIJING AGRO BIOTECH RES CENT
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