Programmable oncolytic virus vaccine system and application thereof

Abstract

The present invention provides an expression system, the system comprising: a first nucleic acid molecule having a cell specific promoter; a second nucleic acid molecule encoding a transcriptional activator; a third nucleic acid molecule having a first recognition sequence of the transcriptional activator; a fourth nucleic acid molecule having a first promoter and a first regulatory element; a fifth nucleic acid molecule encoding a first regulatory protein; a sixth nucleic acid molecule having a second recognition sequence of the transcriptional activator; a seventh nucleic acid molecule having a second promoter and a second regulatory element; an eighth nucleic acid molecule encoding a second regulatory protein; and: a ninth nucleic acid molecule configured to conditionally inhibit expression of the first regulatory protein; and a tenth nucleic acid molecule configured to conditionally inhibit expression of the second regulatory protein, wherein the first regulatory element is adaptedto inhibit the function of the first promoter by binding to the second regulatory protein, and the second regulatory element is adapted to inhibit the function of the second promoter by binding to the first regulatory protein.

Description

[0001] priority information [0002] This application claims the priority and rights of the patent application No. 201710184736.2 filed with the State Intellectual Property Office of China on March 24, 2017, and is hereby incorporated by reference in its entirety. technical field [0003] The present invention relates to the field of biomedicine, specifically, the present invention relates to expression systems and applications thereof, more specifically, the present invention relates to expression systems, recombinant viruses, recombinant cells and the use of expression systems, recombinant viruses, and recombinant cells in the preparation of medicines, Furthermore, the present invention also relates to a method for expressing a target protein using an expression system. Background technique [0004] Oncolytic viruses refer to a class of viruses that have the ability to replicate and package to kill tumors. At present, most studies have transformed some naturally occurring...

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Problems solved by technology

[0007] However, the research results of the existing oncolytic adenoviruses show that the existing regulation methods have defects such as too single, poor specificity, and low safety.

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