Pig endogenous retrovirus vector and construction method thereof

A technology of retrovirus and construction method, which is applied in the field of porcine endogenous retrovirus vector and its construction, can solve problems such as retrovirus vectors that have not yet been seen, and achieve the elimination of doubts about safety and genetic background Clear, broad host range effects
CN101899472AInactive Publication Date: 2010-12-01FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
Publication Date
2010-12-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a pig endogenous retrovirus vector and a construction method and application thereof. The vector constructed in the invention has the following advantages of: efficiently infecting various cells and having wide host range, efficiently integrating a target gene into a genome of the infected cells and expressing in a high level, having efficient infection characteristic, transmitting a recombinant target gene to an entire cell colony, combining with a tissue specificity promoter and probably fulfilling the aims of positioning and expressing the target gene in specific tissues or organs, along with higher suitability for gene therapy and other application.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a retrovirus vector and a construction method thereof, in particular to a pig endogenous retrovirus vector and a construction method thereof. Background technique

[0002] Retroviral vectors are gene transfer vectors commonly used in clinical gene therapy trials. Compared with other non-viral vectors and viral vectors, retroviral vectors have many unique advantages, including high-efficiency transduction of various types of cells, and ability to integrate into hosts. The exogenous gene carried by it is stably expressed in the chromosome of the cell for a long time. Most of the structural protein coding sequences gag, pol, and env genes are removed from the retroviral vectors currently used by people, so that the virus loses the ability to replicate and form virions.

[0003] These three viral structural proteins need to be included in the vector packaging system by constructing co-tra...

Claims

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