Platelet lysate supported micro-sphere preparation method

A technology of platelet lysate and platelet, which is applied in the direction of blood/immune system cells, cells fixed on/in organic carriers, bone/connective tissue cells, etc. It can solve the problem of large consumption of platelet lysate, poor adsorption effect of microcarriers, etc. problem, to achieve the effect of effective proliferation, promotion of attachment, and increase of specific surface area

Active Publication Date: 2018-10-30
天晴干细胞股份有限公司
View PDF54 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a method for preparing microspheres loaded with platelet lysate in order to solve the problem of large consumption of platelet lysate or poor adsorption effect of microcarriers in the existing cell culture

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Platelet lysate supported micro-sphere preparation method
  • Platelet lysate supported micro-sphere preparation method
  • Platelet lysate supported micro-sphere preparation method

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0024] Specific embodiment one: In this embodiment, a method for preparing microspheres loaded with platelet lysate is carried out according to the following steps:

[0025] 1. Add basement membrane proteoglycan solution to the platelet lysate rich in CD41+ and CD81+ microcapsules, and stir evenly while adding to obtain composition A; wherein the platelet lysate and basement membrane protein rich in CD41+ and CD81+ microcapsules The volume ratio of polysaccharide solution is (3.7-5.5):(0.8-2.4);

[0026] 2. At room temperature, add the sodium alginate solution to composition A, and mix well to form composition B; wherein the volume ratio of the sodium alginate solution to the platelet lysate rich in CD41+ and CD81+ microcapsules is (3.0- 5.0):(3.7-5.5);

[0027] 3. Add composition B to a spray gun equipped with a porous nozzle, use nitrogen as the power, and spray into 3-10% CaCl at 4°C with 90rpm magnetic stirring at a speed of 1-30m / s 2 In aqueous solution, microspheres wi...

specific Embodiment approach 2

[0032] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the preparation method of the platelet lysate rich in CD41+, CD81+ microcapsules in step one is:

[0033] 1. Use a focused extracorporeal shock wave instrument with a frequency of 15Hz and an energy-flux density of 0.15-0.20mJ / mm 2 , after shocking platelet-rich plasma or platelet-rich saline 200-500 times, at 37°C CO 2 Incubate in the incubator for 10-15 minutes; then at a frequency of 15Hz, the energy flux density is 0.20-0.25mJ / mm 2 , shock 700-1000 times, at 37°C CO 2 Incubate in the incubator for 20-30 minutes; then at a frequency of 15Hz, the energy flux density is 0.25-0.30mJ / mm 2 , shock 1500-2000 times, at 37°C CO 2 Incubate in an incubator for 10 minutes to obtain a suspension;

[0034] 2. Put the suspension in step 1 into a sealed freezing tube, put it into liquid nitrogen to freeze for 20-40 minutes, take it out and put it in a water bath to shake and melt, re...

specific Embodiment approach 3

[0036] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 or 2 is that the platelet concentration in platelet-rich plasma or platelet-rich saline is (6-15)×10 5 pieces / μl. It is the same as the specific embodiment 1 or 2.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
concentrationaaaaaaaaaa
Login to view more

Abstract

The invention relates to a platelet lysate supported micro-sphere preparation method, and aims to solve the problem of large platelet lysate consumption or poor micro-carrier adsorption effect in existing cell culture. The preparation method includes the steps of platelet lysate preparation, micro-sphere component preparation and platelet factor and micro-capsule supported micro-sphere preparation. A prepared micro-sphere is quite strong in osteoblast carrying capacity, most of cells are fitted on the micro-sphere under the condition that the ratio of osteoblasts to the micro-sphere is 500:1,and only few individual cells are scattered in culture solution. Mesenchymal stem cells on the micro-sphere are good in growth condition, cell viability exceeds 95%, doubling time is short, positive expression rate exceeds 95% in terms of purity, and the preparation method is applied to the technical field of biology.

Description

technical field [0001] The invention relates to a method for preparing microspheres loaded with platelet lysate. Background technique [0002] Platelets have been regarded as non-functional cell fragments in the blood for a long time. It was not until 1882 that the Italian physician Bizzozero discovered that they played an important role in the hemostasis process after vascular injury, and it was first proposed that the name of platelets began. Explore the process of platelet coagulation, hemostasis, and maintenance of capillary wall integrity. It is now recognized that platelets have important functions. [0003] Platelets are biologically active small pieces of cytoplasm that are lysed and shed from the cytoplasm of mature megakaryocytes in the bone marrow. They are small in size, 2-3 microns in diameter, and have no nucleus. Platelets have a specific morphological structure and biochemical composition, and have a relatively constant number in normal blood (for example, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C12N5/078C12N11/10
CPCC12N5/0644C12N5/0665C12N11/10
Inventor 张怡吕秀明牛春玮刘艳青
Owner 天晴干细胞股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap