Platelet lysate supported micro-sphere preparation method

A technology of platelet lysate and platelet, which is applied in the direction of blood/immune system cells, cells fixed on/in organic carriers, bone/connective tissue cells, etc. It can solve the problem of large consumption of platelet lysate, poor adsorption effect of microcarriers, etc. problem, to achieve the effect of effective proliferation, promotion of attachment, and increase of specific surface area
CN108715833AActive Publication Date: 2018-10-30天晴干细胞股份有限公司
54 Cites 4 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
天晴干细胞股份有限公司
Publication Date
2018-10-30

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a platelet lysate supported micro-sphere preparation method, and aims to solve the problem of large platelet lysate consumption or poor micro-carrier adsorption effect in existing cell culture. The preparation method includes the steps of platelet lysate preparation, micro-sphere component preparation and platelet factor and micro-capsule supported micro-sphere preparation. A prepared micro-sphere is quite strong in osteoblast carrying capacity, most of cells are fitted on the micro-sphere under the condition that the ratio of osteoblasts to the micro-sphere is 500:1,and only few individual cells are scattered in culture solution. Mesenchymal stem cells on the micro-sphere are good in growth condition, cell viability exceeds 95%, doubling time is short, positive expression rate exceeds 95% in terms of purity, and the preparation method is applied to the technical field of biology.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a method for preparing microspheres loaded with platelet lysate. Background technique

[0002] Platelets have been regarded as non-functional cell fragments in the blood for a long time. It was not until 1882 that the Italian physician Bizzozero discovered that they played an important role in the hemostasis process after vascular injury, and it was first proposed that the name of platelets began. Explore the process of platelet coagulation, hemostasis, and maintenance of capillary wall integrity. It is now recognized that platelets have important functions.

[0003] Platelets are biologically active small pieces of cytoplasm that are lysed and shed from the cytoplasm of mature megakaryocytes in the bone marrow. They are small in size, 2-3 microns in diameter, and have no nucleus. Platelets have a specific morphological structure and biochemical composition, and have a relatively constant number in normal blood (for example, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More