9 results about "Doubling time" patented technology

An object of the present invention is to provide a method for improving a proportion of capsids containing a gene of interest GOI in a method for producing a virus, and to provide a method for improving a proportion of capsids containing a gene of interest GOI in a method for introducing a nucleic acid into cells, the method including a nucleic acid introduction step of adding a nucleic acid to a suspension containing cells. According to the present invention, there is provided a method for producing a virus, the method including a nucleic acid introduction step of adding a nucleic acid to a suspension containing cells having a density of 10×106 cells / mL or more, and a culture step of culturing the cells into which the nucleic acid has been introduced, in which the nucleic acid is added in a case where a population cell doubling time of the suspension satisfies 35.4 hours or less.

The invention relates to the technical field of biology, in particular to the technical field of formic acid biological utilization and efficient biological conversion. The invention provides a formic acid biotransformation engineering strain. The bacterial strain is obtained after a plasmid pED31-FFM and a plasmid pAD31-gcvTHP are introduced into escherichia coli, and the plasmid pED31-FF M is obtained after an ftfl-fchA-mtdA (FFM) gene is connected to a pED31 empty plasmid; the plasmid pA D31-gcvTHP is obtained by connecting a gcvTHP gene to a pAD31 empty plasmid, and the plasmid pA D31-gcvTHP can be obtained by connecting the gcvTHP gene to the pAD31 empty plasmid. According to the strain provided by the invention, under the initial concentration of 20-60mM formic acid, the multiplication time is within 5 hours, the biomass yield can reach 6g CDW / mol formic acid, the single-cell protein yield can reach 2.45-4.2 g / mol, and the international leading level of formic acid conversion and biomass is reached.

To provide an improved serum-free animal cell culture medium that can be used for production of a protein of interest.SOLUTION: Inclusion of ornithine, either with or without putrescine, in serum-free cell culture medium ("OS" medium) increases cell viability and density, decreases cell doubling time, and allows for high titer protein production by these cells.SELECTED DRAWING: None

Methods and systems for antibacterial susceptibility testing of a bacterium are provided. The method includes exposing a bacterium to an antimicrobial agent. A series of images of the bacterium is captured over time after exposure The series of images are captured during an imaging period. For each image of the series of images, the method includes extracting a value of each feature in a set of morphological features of the bacterium. The set of morphological features includes one or more of area, aspect ratio, length, circularity, perimeter, angularity, curvature, ferret, pole, roundness, sinuosity, width, trajectory, morphology, orientation, solidity, and z-score. A rate of change is calculated for each feature of the set of morphological features during the imaging period. An inhibition status of the bacterium is determined using a machine-learning classifier applied to input data.

The invention discloses a chicken SERBP1 gene knockout cell line as well as a construction method and application thereof, and belongs to the technical field of gene engineering. The invention provides sgRNA for knocking out a chicken SERBP1 gene, the sequence of the sgRNA is shown as SEQ ID NO.1, a CRISPR-Cas9 system is prepared according to the sgRNA, and a chicken SERBP1 gene knockout cell line is constructed. Experimental results show that after the SERBP1 gene is knocked out, replication of IBDV in the cell line is remarkably promoted, the chicken SERBP1 gene knockout cell line is high in adaptability, and the cell multiplication time is 24 h; the SERBP1 gene can still be stably silenced after the cells are subcultured to 10 generations, and no SERBP1 protein is expressed. The invention provides a new cell line model for producing the IBDV virus, researching the mechanism of the host against the IBDV and preparing the medicine for preventing and treating the IBDV.

The application provides a fusion double-time-scale pulse minimum gate method and an acceleration device, and the method comprises the following steps: S1, obtaining input features of a current outer time step, performing a fusion linear transformation combined with output pulses of a previous outer time step, generating a linear transformation result and generating a single gate signal based on the result; S2, adopting a double-time-scale attenuation mechanism, maintaining a cross-frame long-time memory membrane potential with an outer attenuation factor and responding to an instantaneous input in a frame with an inner attenuation factor; S3, based on the gate signal, weighting and fusing the maintained membrane potential and the responsive membrane potential to obtain a current fusion membrane potential; S4, comparing the fusion membrane potential with a preset threshold value, generating an output pulse and resetting. The application solves at least one of the technical problems of high inference delay, frequent weight memory, high hardware cost of the gate unit, large area occupied by the multiplier, single time scale structure limitation and complex training process in the existing pulse neural network timing reasoning scheme.

The application provides an agent memory auditing method and device based on a double time axis and a storage medium. The method comprises: receiving memory data associated with a user identifier, and recording a first time parameter and a second time parameter for each piece of memory data; performing a double time axis joint determination on the memory data based on a target time point to determine an effective memory data set; when new memory data is received, performing state invalidation updating on existing memory data corresponding to the new memory data, the state invalidation updating comprising updating the end time of the first time parameter of the existing memory data to the start time of the first time parameter of the new memory data in the same transaction, and writing the new memory data to form a state evolution record; generating an audit result based on the effective memory data set, and recording an audit log corresponding to an audit request. The application can improve the memory time semantic distinction ability, improve the historical state backtracking accuracy, and enhance the audit tracing integrity.

The invention discloses a project progress management system based on a double-time-mark code name network diagram, and the system defines a non-critical task time difference as a dynamic elastic interval, dynamically adjusts an interval boundary through combining upstream delay, resource conflicts and external interference data, and outputs real-time elastic interval data; by calculating an elastic interval width attenuation rate, when the attenuation rate exceeds a safety baseline threshold value, triggering early warning of a corresponding path, and outputting a buffer health index and an early warning level; constructing a time difference consumption chain model, inputting local progress deviation, then simulating time difference loss of subsequent tasks, displaying paths and high-risk areas on a double-time-scale network diagram in a pulse animation and thermodynamic diagram manner, outputting a cascade risk report, and screening out high-consumption-rate paths; and traversing tasks of which the elastic interval does not reach an early warning threshold in the path, generating a translation scheme, and after logic and resource constraint detection, outputting a feasibility scheduling instruction and a corresponding risk mitigation rate.

The invention provides engineering vibrio natriticus for producing farnesene by using ethanol and a preparation method of farnesene, and relates to the technical field of bioengineering. In the embodiment of the invention, traditional chassis organisms such as yeast which grow slowly are abandoned, and the vibrio natriticus of which the doubling time is less than 10 minutes is used as a chassis; and engineering vibrio natrieti capable of efficiently utilizing ethanol is obtained by combining directed evolution, so that the thallus proliferation and fermentation period is greatly shortened. The selected exogenous plasmids Kesling 2187 and pFZ71 carry a gene cluster with a definite function, and after the gene cluster is successfully introduced into the vibrio natriticus, engineering vibrio natriticus with stable inheritance is formed. MVA pathway key genes (atoB, tHMG1 and the like) carried by the exogenous plasmids and a farnesene terminal synthesis gene (AFS1) have a synergistic effect, so that a complete and efficient synthesis pathway from ethanol to acetyl coenzyme A to FPP to alpha-farnesene is formed, the metabolic flux loss is reduced, and the farnesene synthesis flux is improved.