136 results about "Salmonella dan" patented technology

The invention provides an Enterococcus faecium ANSE228 of which the collection number is CGMCC No.4082. The invention also provides application of the Enterococcus faecium ANSE228 to inhibition of salmonella pullorum and/or Escherichia coli and/or Staphylococcus aureus. The Enterococcus faecium ANSE228 is obtained by processes of repeated separation, purification, rejuvenation and the like, and has high biological activity, obvious probiotic property, high adversity resistance and the like. The invention also provides a microecological agent which contains the Enterococcus faecium ANSE228. When the microecological agent is added into drinking water and/or feeds for breeding animals, the Enterococcus faecium ANSE228 can be quickly activated and reproduced and a dominant beneficial flora can be formed after the Enterococcus faecium ANSE228 is fed into intestinal canals of the animals, and the Enterococcus faecium ANSE228 has the effects of reducing a harmful flora in the intestinal canals, adjusting microecological balance of the intestinal canals, substituting for medicaments such as antibiotic and the like, and improving weight increment of the animals and the utilization rate of the feeds.

The preparation method of girasole beverage includes three technological process steps: 1. preparation of girasole hydrolyzate; 2. preparation of osmanthus flower extract; and 3. blending, packaging and sterilizing. The girasole hydrolyzate, osmanthus flower extract, phosphoric acid and citric acid are mixed according to the mixing ratio of 100:(10-12):3:(4-6), then water is added according to the ratio of the above-mentioned mixed material liquor and water of 1:(50-60), uniformly stirred, bottled, capped and sterilized so as to obtain the invented product with rice nutrients and several health-care functions of reproducing bifidobacteria in human body, inhibiting the growth of intestinal salmonella and spilage organism, raising immunity of human body and reducing blood-lipid, etc.

Antimicrobial salt solutions for food safety and quality applications are disclosed. Because these solutions contain a substantial concentration of salt, they are adaptable to a variety of food-processing applications, such as for chilling brine applications, disinfecting meat baths/rinses, beef injection brines, poultry chill tanks, brines used in cheese manufacture, as a wash to kill salmonella on hard-boiled eggs, and as a wash to disinfect produce, which can become contaminated with salmonella and other pathogenic bacteria in the field. These make use of concentrated salt solutions that depress the freezing point of the solution, to provide a low temperature bath or shower in which food products can be cooled. A preferred formulation contains between 25 ppm and 25,000 ppm surfactant, between 0.1% acid and 25% acid, and between 72.5% and 99.9% salt. This blend can then be dissolved in water to make a solution of between about 1% total solids by weight up to the saturation point, which can be used as an antimicrobial solution for food safety applications. An even more preferred range of solutions would be 17% by weight of between 0.3% and 6.0% citric acid, between 50 and 500 ppm SLS, and between 94% and 99.7% sodium chloride, and can be used in process temperatures of about -6.7° C.

The present invention provides a gene chip for detecting common pathogen in dairy, which comprises a solid-phase vector and an oligonucleotide probe fixed on the solid-phase vector, wherein the oligonucleotide probe includes DNA fragment or its complementary DNA or RNA sequence selected from E. sakazakii, streptococcus pyogenes, staphylococcus aureus, Klebsiella pneumoniae, Klebsiella oxytoca, Listeria monocytogenes, salmonella and 16S-23S rDNA intergenic spacer region of Bacillus cereus, as well as ipaH gene of Shigella or gyrB gene of citrobacter freundii. The invention further provides a reagent box which uses the gene chip to detect pathogen in dairy. The gene chip and the reagent box of the invention for detecting pathogen in dairy are easy to operate, highly precise and greatly repeatable.

The present invention provides an edible film and film solution comprising incorporated organic acids; protein and glycerol useful for coating raw whole fruit, fresh cut fruit, vegetables, meat, poultry, seafood, cereals, nuts, etc. Moreover, the edible films of the present invention can inhibit pathogen growth including Listeria monocytogens, Salmonella gaminara and E. coli 0157:H7. In a preferred embodiment, the edible film comprises 0.9% glycerol; 10% soy protein; and 2.6% malic acid. The present invention also provides a method for coating comestible products with edible films without masking the color but increasing the shelf-life.

The invention relates to a health-care beverage. The health-care beverage is prepared from the following raw materials in parts by weight: 50-99.99999999 parts of water, 0-30 parts of prebiotics and 0.00000001-20 parts of an additive. The health-care beverage disclosed by the invention is prepared from the water, the prebiotics and the additive in set parts by weight; in addition, the water is used as the main component, so that the cost of the health-care beverage is lower, the health-care beverage is suitable for people to drink for a long term, and side effects do not exist; the prebiotics can regulate a pH value in intestines, restrain the growth of salmonella and putrefying bacteria in the intestines, regulate gastroenteric functions and maintain microecological balance; and the additive can enable the health-care beverage to have various efficacies. The health-care beverage disclosed by the invention has the advantages that the health-care beverage is low in cost and convenient to popularize, the microecological balance of a human body can be fundamentally regulated, the gastroenteric functions can be regulated, the immunity and the resistibility of an organism are enhanced, and the absorption of the human body to nutrient substances is promoted.

The present invention provides a micro-gap electrode array immune sensor which is used for detecting food-borne causal agent. The sensor comprises a micro-gap array electrode, an electrode substrate which is silylanized, an enzyme deposit which has excellent electric conductivity and an enzyme linked immunoreaction sensor based on the electrical conducting test. The invention also provides a detecting method. The invention uses the micro-gap electrode array immune sensor for detecting salmonella and bacillus coli O157:H7. The method has the advantages of high sensitivity, easy operation, portability and low cost. The invention can provide an immune detecting technique which has the advantages of high speed, practicability, low cost, high sensitivity and high flux for detection and determination of the food-borne pathogenic bacteria. The transmission of food-borne pathogenic bacteria can be controlled and prevented in time and effectively.

The invention provides a broad-spectrum nucleic acid aptamer capable of identifying lipopolysaccharides of different Gram-negative bacterium sources. The broad-spectrum nucleic acid aptamer is obtained by directed screening on the basis of the capture-SELEX technology of label-free target molecules and immobilized nucleic acid libraries. A directed screening method of the broad-spectrum nucleic acid aptamer includes: using mouse salmonella typhi lipopolysaccharides as the unique target, using a biotin-avidin effect to fix random oligonucleotide libraries to Fe3O4 magnetic nano particles wrapped by avidin through biotinylated short complementary chains, performing incubation, separation, amplification, digestion single chain preparation, cloning sequencing and the like, adding complete active bacteria of salmonella and escherichia coli to serve as directed molecules to perform directed screening when the libraries are enriched to a certain degree, and one broad-spectrum nucleic acid aptamer capable of identifying four kinds of lipopolysaccharides is obtained through 15 rounds of repeated screening. The nucleic acid aptamer is applicable to the analysis and detection, separation and enriching, toxicity neutralizing and the like of the lipopolysaccharides in aspects of drinking water, food or clinical medicine and the like.

A method of extracting buckwheat flavone from buckwheat hull includes: shatter sweet buckwheat hull or tartary buckwheat hull raw material, formulate extracting solvent of 0.1 mol per liter sodium carbonate, use ultrasonic to extract, use kieselguhr filter for filtration, deposit, centrifuge, washing, and dry craft steps. By substantive laboratory studies and test, the heavy metal content, flavacin B1, pesticide residue, total bacterial count, mold and microzyme number, coliform group, salmonella and Staphylococcus aureus of buckwheat flavone produced by this method all meet regulation of commerce department medicinal plant and preparation trade green industry stand ard.

The invention discloses a maturative agent for a hawk tea golden flower cheese, and a preparation method of the maturative agent. The maturative agent for a hawk tea golden flower cheese consists of a tea polysaccharide concentrate of a hawk tea, and eurotium cristatum spores, wherein each gram of the maturative agent for the hawk tea golden flower cheese contains 1*1010-6*1010 eurotium cristatum spores. The maturative agent for the hawk tea golden flower cheese, disclosed by the invention, can be used for maturing mucedine cheeses, and has the unique perfume of the hawk tea and the golden color of the eurotium cristatum thalli. According to the maturative agent for the hawk tea golden flower cheese, disclosed by the invention, the kinds of the maturative agent for cheeses and the variety of maturative cheeses are enriched, and besides, harmful microorganisms, such as coliform, golden staphylococcus, salmonella and listeriosis can be retrained from breeding in the cheeses.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum and Salmonella pullorum, a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella enteritidis or Salmonella typhimurium, Fowl typhoid caused by Salmonella gallinarum, and Pullorum disease caused by Salmonella pullorum, which comprises the bacteriophage as an active ingredient, and an animal feed, drinking water, cleaner, and sanitizer which comprise the bacteriophage as an active ingredient.

The invention discloses a lactobacillus plantarum SWUN5815 for high-yield bacteriocin and an application of the lactobacillus plantarum SWUN5815. The strain is preserved at the China Center for Type Culture Collection (CCCTCC) on August 2, 2016 and the preservation number is CCTCC NO: M2016419; and the strain is separated from yak dung. The bacteria generated by the strain can be degraded by protease; the strain has a very high inhibition effect on salmonella and escherichia coli and has good acid resistance and relatively high cholate tolerance; the survival rate after 3h in artificial gastric juice of which the pH is 3.0 is 93.36+/-4.06%; the strain can slowly grow in 1.0% cholate; and the growth efficiency reaches 15.26+/-1.19% of that of the strain which is cultivated under the condition free of the cholate. The bacteriocin is capable of effectively inhibiting growth of salmonella, escherichia coli and staphylococcus aureus. Therefore, the lactobacillus plantarum SWUN5815 for the high-yield bacteriocin has the potential of being applied to yak intestinal tracts as a microecological preparation.

The invention discloses salmonella enteritidis detection reagent kit and method based on a loop-mediated isothermal amplification technology, wherein two specific inner primers and two specific outer primers are designed by the reagent kit according to the six specific regions of a salmonella enteritidis Sdf I gene conserved region, thereby ensuring the specificity of loop-mediated isothermal amplification and the reliability of a detecting result; in the invention, salmonella enteritidis is detected on the basis of the loop-mediated isothermal amplification technology, a target sequence can be rapidly, efficiently and specifically amplified under an isothermal condition, the operation is simple and convenient, expensive instruments and reagents are not needed, an amplified product is directly developed through fluorescent dye, a result can be judged by naked eyes, and the detection cost is low; and the reagent kit and the method can specifically distinguish serum specific type salmonella enteritidis and other serum type salmonella and are particularly suitable for detection and application in medium and small units and fields.

Disclosed herein is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum, and Salmonella pullorum without affecting beneficial bacteria, in addition to showing excellent tolerance to acid, heat and desiccation. The novel bacteriophage of the present invention can be widely used as an active ingredient for therapeutic agents, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum or Salmonella pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the Salmonella bacteria. The present invention also provides important insights into prevention and control strategies against Salmonella infection and suggests that the use of bacteriophage can be a novel, safe, and effectively plausible alternative to antibiotics for the prevention of Salmonella infection in poultry.

The invention discloses an electrochemical immunosensor. An antibody for resisting phosphothreonine lyase is at the center of the electrode surface of the electrochemical immunosensor, and is preferably a monoclonal antibody; a sensor preferably contains a double-layer nano-gold membrane; and an electrode of the electrochemical immunosensor is preferably a glass-carbon electrode. The phosphothreonine-lyase electrochemical immunosensor modified by the double-layer nano-gold is constructed by taking chitosan as a bridging agent, fixing a first layer of nano-gold on glass-carbon electrode and adsorbing and fixing the antibody for resisting phosphothreonine lyase, fixing a thionine-chitosan /nano-gold-horseradish peroxidase (HRP) complex to the electrode by dropping onto the electrode, and adsorbing the antibody for resisting phosphothreonine lyase. The electrochemical immunosensor is applicable to detect salmonella and/or shigella in food, is high in sensitivity and strong in specificity, and is capable of realizing rapid quantitative detection on salmonella and/or shigella.

A test kit and test apparatus are provided for the detection of enzymes excreted by broken or ruptured bacteria cells in various environments, such as in the field, soil extracts, produce washing, slaughter houses, food preparation area's and other related or non-related areas where a quick test or rapid test is needed to obtain qualitative results. The method utilizes various components which allow for the collection of a sample from suspected contaminated areas. One method is to collect a sample from a pond or other water source possibly contaminated and by using a clean lab sample tube or clean plastic bag, a field hand mixer, a buffer, a pipette and a test strip with a reagent pad.