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Enzyme catalysis conductance immune sensor and method for detecting food-borne causal agent

An immunosensor, food-derived technology, applied in the field of biological analysis, can solve the problems of low specificity, low sensitivity, cumbersome and time-consuming operation, etc., and achieve the effect of simple detection steps, high sensitivity, and simple preparation

Inactive Publication Date: 2008-10-01
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the shortcomings of traditional food-borne pathogen detection technology such as low sensitivity, low specificity and cumbersome and time-consuming operation, and propose an enzyme-catalyzed conductance immunosensor and its method for detecting food-borne pathogens, which have the advantages of High sensitivity, good specificity, fast and low cost

Method used

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  • Enzyme catalysis conductance immune sensor and method for detecting food-borne causal agent

Examples

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Effect test

Embodiment 1

[0029] Example 1: Detection of Salmonella by an immunosensor based on microgap array electrodes.

[0030] 1. Preparation of immunosensor:

[0031] The micro-gap array gold electrode produced by the conventional photolithography printing method is used as the substrate, which is an interdigitated double electrode, and the positive and negative electrodes are two comb-shaped gold electrodes, which cross each other to form a micro-gap array gold electrode. Comb tooth width is 1μm-100μm, gap is 1μm-100μm;

[0032] After washing the microgap array gold electrode with absolute ethanol (100%) three times (1 min each time), the electrode was immersed in 1M NaOH solution for 30 min; then the electrode was washed with ultrapure water three times (1 min each time), and dried; Then, the electrode is immersed in an ethanol solution containing 5% (volume percentage) of aminopropyltrimethoxysilane, and left to stand at room temperature for 24 hours; it is washed with ultrapure water three t...

Embodiment 2

[0037] Example 2: Detection of Escherichia coli O157:H7 by an immunosensor based on micro-gap array electrodes.

[0038] 1. Preparation of immunosensor:

[0039]The micro-gap array gold electrode produced by the conventional photolithography printing method is used as the substrate, which is an interdigitated double electrode, and the positive and negative electrodes are two comb-shaped gold electrodes, which cross each other to form a micro-gap array gold electrode. Comb tooth width is 1μm-100μm, gap is 1μm-100μm;

[0040] After washing the microgap array gold electrode with absolute ethanol (100%) three times (1 min each time), the electrode was immersed in 1M NaOH solution for 30 min. Then wash the electrode three times with ultrapure water (each time for 1 min), and dry it. Then, the electrode is immersed in an ethanol solution containing 5% (volume percentage) of aminopropyltrimethoxysilane, and left to stand at room temperature for 24 hours; it is washed with ultrapure...

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Abstract

The present invention provides a micro-gap electrode array immune sensor which is used for detecting food-borne causal agent. The sensor comprises a micro-gap array electrode, an electrode substrate which is silylanized, an enzyme deposit which has excellent electric conductivity and an enzyme linked immunoreaction sensor based on the electrical conducting test. The invention also provides a detecting method. The invention uses the micro-gap electrode array immune sensor for detecting salmonella and bacillus coli O157:H7. The method has the advantages of high sensitivity, easy operation, portability and low cost. The invention can provide an immune detecting technique which has the advantages of high speed, practicability, low cost, high sensitivity and high flux for detection and determination of the food-borne pathogenic bacteria. The transmission of food-borne pathogenic bacteria can be controlled and prevented in time and effectively.

Description

technical field [0001] The invention belongs to the field of biological analysis, and in particular relates to an immune sensor based on a micro-gap array electrode and a method for detecting food-borne pathogens in food by using the immune sensor. Background technique [0002] Foodborne pathogens refer to a large class of bacteria and viruses that use food as a carrier and cause human diseases. In recent years, global food safety incidents have occurred frequently, such as the "Listeria incident" in the United States and the "Escherichia coli O157 epidemic incident" in Japan, etc., which have brought great harm to human health and attracted the full attention of governments. Traditional detection methods (such as isolation and culture, biochemical identification, etc.) cannot detect pathogenic bacteria that are difficult to cultivate or cannot be cultured, and they have low specificity, low sensitivity, cumbersome and time-consuming operations, and cannot achieve effective ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/569G01N33/535G01N27/327
Inventor 蒋健晖黄勇俞汝勤沈国励楚霞
Owner HUNAN UNIV
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