Enterococcus faecium ANSE228 and application thereof

A technology of Enterococcus faecalis and seeds, applied in Enterococcus faecalis and its application fields, can solve the problems of increasing the occurrence of digestive tract diseases of livestock and poultry, mutation of pathogenic bacteria into drug-resistant strains, and destruction of animal microecological balance, etc., to achieve significant benefit Sex, reduce production cost, improve production performance

Active Publication Date: 2011-04-27
科润生科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its long-term and large-scale use has led to some negative effects, such as destroying the micro-ecological balance of the animal itself, increasing the incidence of digestive tract diseases in livestock and pou...

Method used

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  • Enterococcus faecium ANSE228 and application thereof
  • Enterococcus faecium ANSE228 and application thereof
  • Enterococcus faecium ANSE228 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation of embodiment 1 enterococcus faecalis ANSE228 fermented liquid

[0040] Get Enterococcus faecalis ANSE228 (preservation number is CGMCC No.4082, viable bacteria concentration is 10 9 CFU / ml), inoculated in 50ml medium for shake flask fermentation culture, the fermentation temperature was 37°C, the pH value was 7.0, static culture, and the fermentation time was 24h.

[0041] Among them, the shake flask fermentation medium is composed of the following components: corn flour 15g, soybean meal 20g, glucose 5g, dipotassium hydrogen phosphate 0.15g, potassium dihydrogen phosphate 1.5g, manganese sulfate monohydrate 1.0g, magnesium sulfate heptahydrate 1.5 g, distilled water 1000mL, pH value is 7.0.

[0042] After the shake flask fermentation, carry out the fermenter pilot test, take 50ml of shake flask fermentation seed liquid and inoculate it into a 10L fermenter, the liquid volume is 5L, the fermentation temperature is 37°C, the pH value is 7.0, the stirrin...

Embodiment 2

[0044] Example 2 Probiotic verification of Enterococcus faecalis ANSE228

[0045] Prepare an MRS plate with a thickness of about 4mm, and make the concentration of pathogenic bacteria (Escherichia coli, Staphylococcus aureus and Salmonella) 10 6 Spread 100 μL of the bacteria suspension on the plate, and place a sterilized stainless steel small tube (round small tube with an inner diameter of 6 mm, an outer diameter of 8 mm, and a height of 10 mm, with smooth ends) placed on the medium by aseptic operation. , gently pressurized so that it contacts with the culture medium without gaps, and after a few minutes, add 200-300 microliters of the fermented liquid (activated strain bacterial liquid) prepared in Example 1 dropwise to each small tube respectively. Make it overflow, incubate at 37°C for 24 hours, and then measure the diameter of the inhibition zone. Each experiment was repeated three times and the average value was taken. The result is as Figure 1-3 shown.

[0046] W...

Embodiment 3

[0048] Example 3 Stress resistance verification of Enterococcus faecalis ANSE228

[0049] 1. Take the preserved 5mL fermented liquid prepared in Example 1 and pour it into the centrifuge tube 1, use ten-fold serial dilution, and spread it on the MRS plate 1. Then put the centrifuge tube 2 containing 5mL of the fermented liquid prepared in Example 1 in a water bath at 80°C and heat it for 15 minutes, take the heated Enterococcus faecalis bacterial liquid and carry out ten-fold stepwise dilution, and spread it on the MRS plate 2 . Finally, the plates before and after heating were cultured at 37° C. for 24 hours, and the number of Enterococcus faecalis ANSE228 before and after heating was calculated.

[0050] The results showed that the survival rate of live bacteria reached 85%.

[0051] 2. Tolerance test of simulated gastric juice: Dissolve pepsin in 0.5% normal saline to make the final concentration 3g / L, and adjust the pH value to 2.0 with concentrated hydrochloric acid or ...

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Abstract

The invention provides an Enterococcus faecium ANSE228 of which the collection number is CGMCC No.4082. The invention also provides application of the Enterococcus faecium ANSE228 to inhibition of salmonella pullorum and/or Escherichia coli and/or Staphylococcus aureus. The Enterococcus faecium ANSE228 is obtained by processes of repeated separation, purification, rejuvenation and the like, and has high biological activity, obvious probiotic property, high adversity resistance and the like. The invention also provides a microecological agent which contains the Enterococcus faecium ANSE228. When the microecological agent is added into drinking water and/or feeds for breeding animals, the Enterococcus faecium ANSE228 can be quickly activated and reproduced and a dominant beneficial flora can be formed after the Enterococcus faecium ANSE228 is fed into intestinal canals of the animals, and the Enterococcus faecium ANSE228 has the effects of reducing a harmful flora in the intestinal canals, adjusting microecological balance of the intestinal canals, substituting for medicaments such as antibiotic and the like, and improving weight increment of the animals and the utilization rate of the feeds.

Description

technical field [0001] The invention relates to an enterococcus faecalis and its application. Background technique [0002] With the development of society and science, people pay more and more attention to food safety, because unsafe food not only endangers human health and life, but may even have adverse effects on future generations. The society calls for green and safe food, and the safety of feed is the safety of food. [0003] Antibiotics, as feed additives, play an important role in promoting animal growth and development, and improving disease resistance. However, its long-term and large-scale use has led to some negative effects, such as destroying the micro-ecological balance of animals, increasing the incidence of digestive tract diseases in livestock and poultry, and causing pathogenic bacteria to mutate into drug-resistant strains. More importantly, the residues of antibiotics in animals are transmitted to humans through the food chain, bringing hidden dangers...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P31/04A23K1/16C12Q1/02A23K10/18A61K35/741
CPCY02A50/30
Inventor 计成马秋刚高欣范彧李笑樱
Owner 科润生科技发展有限公司
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