The invention discloses a phytase mutant and applications thereof. The sequence of the phytase mutant is represented by SEQ ID No.1. The mutant is obtained through the following mutations of amino acid that encodes the amino acid sequence (SEQ ID No.3) of fig aspergillus phytase: A39E, N40D, S42L, V43S, I44V, P46S, R167G, Q169T, P170N, G171R, Q172A, T252R, S253T, T254S, V255D, D256A, T257S, and K258Q. The phytase mutant can be used in feed additive. A mutant (SEQ ID No.2) of novel phytase gene is obtained. The mutant is effective in a wide pH range, has an ideal heatproof characteristic, is suitable for high temperature granulation, and meets the requirements of feed industry.