Phytase mutant and applications thereof

A technology of mutants and phytase, applied in the field of phytase mutants and its application, can solve the problems of poor heat resistance and unsatisfactory practical application of fig phytase

Inactive Publication Date: 2016-05-11
KUNMING QACTIVE BIOLOGICAL TECH CO LTD
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] The object of the present invention is to provide a phytase mutant and its application, aiming to solve the problem that the heat resistance of fig phytase is poor and unsatisfactory in practical application

Method used

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  • Phytase mutant and applications thereof
  • Phytase mutant and applications thereof
  • Phytase mutant and applications thereof

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Embodiment Construction

[0022] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0023] The present invention compares the sequences and structures of the high heat-resistant Aspergillus fumigatus phytase and Aspergillus fig phytase by computer-aided means, and replaces the fig phytase (black Aspergillus phytase) the amino acid sequence of the corresponding position. The amino acid changes in the amino acid sequence of Aspergillus fig (Aspergillus niger) phytase SEQ ID NO: 3 are as follows: A39E, N40D, S42L, V43S, I44V, P46S, R167G, Q169T, P170N, G171R, Q172A, T252R, S253T, T254S, V255D, D256A, T257S, K258Q. The specific embodiment is to use the fig phytase (Aspergillus niger phytase) gene as a templa...

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Abstract

The invention discloses a phytase mutant and applications thereof. The sequence of the phytase mutant is represented by SEQ ID No.1. The mutant is obtained through the following mutations of amino acid that encodes the amino acid sequence (SEQ ID No.3) of fig aspergillus phytase: A39E, N40D, S42L, V43S, I44V, P46S, R167G, Q169T, P170N, G171R, Q172A, T252R, S253T, T254S, V255D, D256A, T257S, and K258Q. The phytase mutant can be used in feed additive. A mutant (SEQ ID No.2) of novel phytase gene is obtained. The mutant is effective in a wide pH range, has an ideal heatproof characteristic, is suitable for high temperature granulation, and meets the requirements of feed industry.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a phytase mutant and its application. Background technique [0002] Phytic acid widely exists in feed materials such as corn, rice bran, and various cakes. On the one hand, it has a strong anti-nutritional effect, and it combines with metal ions and proteins in the feed to affect the digestion and absorption of these nutrients; Phytic acid molecules contain a large amount of organic phosphorus, which cannot be used in the gastrointestinal tract of animals due to the lack of corresponding enzymes. These organic phosphorus are discharged into the environment with feces, causing environmental pollution. Phytase can hydrolyze phytic acid to generate inositol derivatives and orthophosphoric acid. As a feed additive for monogastric animals, it can promote the utilization of phosphorus by animals, contribute to the growth of livestock and poultry, and improve the efficiency of an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/16C12N15/55C12N1/19C12N15/81A23K20/189C12R1/84
CPCC12N9/16C12Y301/03008
Inventor 黄遵锡苗华彪韩楠玉
Owner KUNMING QACTIVE BIOLOGICAL TECH CO LTD
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