Phytase mutant and applications thereof
A technology of mutants and phytase, applied in the field of phytase mutants and its application, can solve the problems of poor heat resistance and unsatisfactory practical application of fig phytase
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[0022] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0023] The present invention compares the sequences and structures of the high heat-resistant Aspergillus fumigatus phytase and Aspergillus fig phytase by computer-aided means, and replaces the fig phytase (black Aspergillus phytase) the amino acid sequence of the corresponding position. The amino acid changes in the amino acid sequence of Aspergillus fig (Aspergillus niger) phytase SEQ ID NO: 3 are as follows: A39E, N40D, S42L, V43S, I44V, P46S, R167G, Q169T, P170N, G171R, Q172A, T252R, S253T, T254S, V255D, D256A, T257S, K258Q. The specific embodiment is to use the fig phytase (Aspergillus niger phytase) gene as a templa...
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