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The application discloses a kind of Ampelomyces quisqualis rice fermentation extraction compound and preparation method and application thereof, its preparation method is: preparation Ampelomyces quisqualis seed liquid;Preparation rice fermentation medium, Ampelomyces quisqualis seed liquid is used to ferment medium, under 22±2 ℃, natural fermentation 5~60 days, after drying, fermentation dry material is obtained for future use;After sieving, fermentation dry material is crushed and added with water, after heating reflux, centrifugation and filtration supernatant, Ampelomyces quisqualis rice fermentation extract is obtained;Hyaluronic acid or sodium hyaluronate is added to Ampelomyces quisqualis rice fermentation extract, and the compound is obtained after swelling and drying.The application is formed by Ampelomyces quisqualis rice fermentation extract and hyaluronic acid (sodium), and the polymer grid can be synergized and quickly anti-inflammatory and promote wound healing, especially promote the regeneration of normal skin appendages such as hair follicle to heal, effectively improve the repair and cure effect on various skin and mucosa damage, and has no negative effect.

This invention belongs to the field of plant growth regulator technology, and relates to an inducer for improving female flower differentiation in *Xanthoceras sorbifolium*, its preparation method, and its application. The inducer, calculated per liter of aqueous solution, comprises 150-1200 mg of 6-benzyladenine, 10-50 mg of salicylic acid, 100-300 mg of choline chloride, 1.0-5.0 g of urea, 2.0-3.0 g of potassium dihydrogen phosphate, 0.5-2.0 g of boric acid, and 0.5-1.0 mL of emulsifier. This invention constructs a multi-component synergistic system for the directional regulation of flower bud sex differentiation, significantly increasing the number and proportion of female flowers in *Xanthoceras sorbifolium*, and reducing the flower bud abortion rate. Simultaneously, it improves the receptivity of the stigma of female flowers and the subsequent fruit setting rate, increasing yield per unit area, solving the industrial problem of "one fruit per thousand flowers" in *Xanthoceras sorbifolium*, and meeting the core needs of high-yield and high-efficiency cultivation and large-scale industrial development of *Xanthoceras sorbifolium*.

The invention discloses a salmonella choleraesuis three-gene deletion strain without a resistance marker, wherein the preservation number of the salmonella choleraesuis three-gene deletion strain is CCTCC (China Center For Type Culture Collection) M 20242059. The strain successively lacks a virulence regulating gene crp, an important nutrition metabolism gene asd for mediating diaminopimelic acid (DAP) synthesis, and an important carbon metabolism gene manA for mediating and coding mannose-6-phosphate isomerase. The salmonella choleraesuis gene deletion mutant strain is constructed by adopting a suicide vector and SacB reverse inheritance, and a non-resistant three-gene deletion mutant strain is obtained. The three-gene deletion mutant strain is clear in genetic background, free of resistance markers, good in safety and immunoprotectiveness to mice and capable of helping the mice to resist attack of high-lethal-dose salmonella choleraesuis, as the asd gene in the deletion strain is completely deleted in a traceless mode, a balanced lethal system plasmid pya3493-ApxIAN is electrically transformed into the deletion strain, and therefore the expression of the salmonella choleraesuis in the high-lethal-dose salmonella choleraesuis in the high-lethal-dose salmonella choleraesuis. The recombinant strain not only can grow in a DAP-free culture medium, but also can stably express foreign protein ApxIAN, and has a wide application prospect.