The invention discloses a multi-mode station stopping method at an optimized comprehensive cost for urban public transport. The stopping method includes steps of firstly, acquiring passenger travel information, vehicle operating information and route station information; secondly, building a objective function with the lowest comprehensive cost based on passenger travel cost and vehicle operating cost, then building constraint conditional expressions corresponding to the objective function; and thirdly, substituting the passenger travel information, the vehicle operating information and the route station information which are acquired in the first step into the objective function and the constraint conditional expressions which are built in the second step to obtain departure frequency h and stopping modes O, and obtaining a multi-mode station stopping mode with the lowest comprehensive cost. The constraint conditional expressions comprise constraints in aspects of public transport capacity, passenger flow, time points, departure intervals and a load factor. By the method, the constraints in the aspects of the public transport capacity, the passenger flow, the time points, the departure intervals and the load factor and the stopping modes are taken into consideration, so that the passenger travel cost and the public transport operating cost are minimized.