5 results about "Growth Factor Gene" patented technology

The invention belongs to the technical field of experimental animal nutriology and sterile animal culture, and particularly relates to a vitamin compound for prolonging the survival time of sterile zebra fish and a preparation method of the vitamin compound. The vitamin compound is prepared from the following components in parts by mass: 600 to 1000 parts of vitamin A, 4000 to 6000 parts of vitamin C, 10 to 30 parts of vitamin D3, 1500 to 2200 parts of vitamin E, 80 to 120 parts of vitamin K3, 15000 to 25000 parts of compound B vitamins and sterile culture water. The preparation method comprises the following steps: dissolving the vitamin raw materials in sterile culture water, performing ultrasonic assisted dissolution, and then filtering and sterilizing to obtain the vitamin supplement. When the vitamin supplement is used for feeding the sterile zebra fish, the survival time of the sterile zebra fish can be obviously prolonged, and the expression level of growth hormone genes and insulin-like growth factor genes can be up-regulated. The application value of the sterile zebrafish in scientific research is further expanded.

The application discloses a kind of RSP03 recombinant proteins, culture medium for prawn cell in vitro proliferation culture and its culture method, proliferation is prawn cell in vivo proliferation or prawn cell in vitro proliferation;The nucleotide corresponding to RSP03 recombinant protein is as shown in sequence SEQ ID NO.1, and the amino acid sequence of RSP03 recombinant protein is as shown in SEQ ID NO.2.The endogenous growth factor RSP03 gene of South American white prawn is cloned, and expression vector is constructed to express and purify endogenous LvRSPO3 recombinant protein, and the in-vitro culture activity of prawn cell can be extremely significantly enhanced by adding 5~30 ng / mL LvRSPO3 recombinant protein on base medium, can more effectively promote the proliferation and division of prawn cell in vitro, and can be cultured for at least 30 days, to overcome the problem that prawn cell is difficult to divide when in vitro culture, and provide a train of thought for establishing prawn continuous cell line.

The invention discloses an RSPO3 recombinant protein, a culture medium for in-vitro proliferation culture of prawn cells and a culture method of the RSPO3 recombinant protein. The proliferation is in-vivo proliferation of the prawn cells or in-vitro proliferation of the prawn cells; the nucleotide corresponding to the RSPO3 recombinant protein is as shown in a sequence SEQ ID NO.1, and the amino acid sequence of the RSPO3 recombinant protein is as shown in a sequence SEQ ID NO.2. An endogenous growth factor RSPO3 gene of penaeus vannamei boone is cloned, an expression vector is constructed to express and purify an endogenous LvRSPO3 recombinant protein, and 5-30 ng / mL of the LvRSPO3 recombinant protein is added to a basic culture medium, so that the in-vitro culture activity of penaeus vannamei boone cells can be remarkably enhanced, in-vitro proliferation and division of the penaeus vannamei boone cells can be more effectively promoted, and the survival rate of the penaeus vannamei boone cells is increased. The method can be cultured for at least 30 days for a long time, and provides a thought for overcoming the problem that prawn cells are difficult to split during in-vitro culture at present and establishing a prawn continuous cell line.

The present invention relates to a nucleic acid molecule comprising a nucleotide sequence encoding a metabolic protein or a portion thereof, or a sequence having at least 90% sequence identity to said metabolic protein or a portion thereof, a human insulin-like growth factor II (IGFII) gene sequence, and a nucleotide sequence encoding at least one peptide that promotes cellular uptake or transcytosis inserted into said IGFII gene sequence between the nucleotides encoding amino acids 28 and 42 of mature IGFII. The invention further relates to related viral particles, fusion proteins, and uses thereof.

The invention relates to nucleic acid molecules comprising a nucleotide sequence encoding a metabolic protein or a part thereof or a sequence having at least 90% sequence identity to said metabolic protein or part thereof, a human insulin-like growth factor II (IGFII) gene sequence, and a nucleotide sequence encoding at least one peptide that facilitates cellular uptake or transcytosis which is inserted at a location between the nucleotides encoding amino acids 28 and 42 of mature IGFII of said IGFII gene sequence. The invention further relates to related viral particles, fusion proteins and uses thereof.