Small multifunctional biochemical analyzer based on total internal reflection resonance principle

Abstract

The invention discloses a small multifunctional biochemical analyzer based on a total internal reflection resonance principle. The small multifunctional biochemical analyzer mainly comprises a total internal reflection electromechanical coupling system module, a temperature control system module, a communication system module and a control system module. The total internal reflection electromechanical coupling system is used as a main body, and the automatic adjustment of the optical angle can be realized through the driving of a single motor, so that the incident light and the optical receiving device can change simultaneously within the angle range of 25-80 degrees. The prism coupling type total internal reflection resonance technology is adopted, angle modulation, intensity modulation and wavelength modulation are achieved, and spectrum detection, fluorescence detection and the like are carried out. In addition, technologies such as a micro electro mechanical system (MEMS), fluorescence detection, total internal reflection, surface plasmon resonance and polymerase chain reaction (PCR) are combined, and multifunctional qualitative and quantitative detection integrating amplification and detection is achieved. The volume of the device is reduced, the nucleic acid molecule amplification time is shortened, the detection efficiency is improved, and the biological reaction is observed in real time.

Description

technical field [0001] The invention relates to the technical field of optical sensing, in particular to label-free quantitative biochemical analysis based on the principle of total internal reflection. Background technique [0002] Total internal reflection resonance sensing technology uses the resonance principle of evanescent wave and surface plasmon wave to detect the interaction between molecules to detect the change process of the optical parameters of the sensing medium, so as to obtain kinetic information such as analyte concentration and affinity. This method is particularly suitable for studying interactions between molecules. Compared with traditional biochemical analysis techniques, total internal reflection resonance sensing technology has the advantages of real-time monitoring, label-free, fast response, and high throughput. It can be seen that the total internal reflection resonance sensing technology has high application value in the field of biochemical det...

AI Technical Summary

Problems solved by technology

[0004] At present, the most widely used structure of total internal reflection resonance sensing device is the prism coupling type, which has four modulation methods: intensity modulation, angle modulation, and phase modulation and wavelength modulation. Compared with the other three commonly used detection methods, phase modulation requires more requirements. It is more complex and not suitable for miniaturization. The commercial total internal reflection resonance detection device is bulky and expensive

At present, most of the total internal reflection detection instruments adopt a modulation detection method. Since each modulation detection application range has its own limitations, different detection methods cannot be applied to different samples to be tested.

[0005] In addition, the existing angle modulation adopts the method of changing the incident angle and reflection angle symmetrically at the same time, but in reality, due to reasons such as prism refraction, the angle values ​​of the incident angle and reflection angle have deviations.

Images