38 results about "Biologic response" patented technology

Compositions and methods disclosed herein relate to improved in vitro cell-free systems for various biological reactions. In one aspect, a composition of the present disclosure includes: a cell-free extract derived from an organism; a nucleic acid; and an organelle that is heterologous to the organism.

The application discloses a kind of biological reaction devices of plant tissue, comprising, culture device, for the culture of plant tissue, the outer wall of the culture device is provided with detection device, for detecting the parameter and / or sampling of culture solution in the culture device;Heat transfer structure, for the temperature control of the culture device, the heat transfer structure has notch, the detection device is arranged on the outer wall of the culture device in the notch.The plant tissue is cultured by the culture device in the application, the temperature in the device is kept stable by heat transfer structure, prevent the influence caused by external temperature to the environment in the device.And detection device is arranged on the outer wall of the culture device in the notch of heat transfer structure, can avoid the damage or heat loss of heat transfer structure caused by detection device passing through heat transfer structure, ensure the integrity of heat transfer structure, make temperature keep stable, plant tissue better growth.

The utility model relates to the technical field of microalgae continuous culture, specifically discloses a continuous culture system and continuous culture method of isochrysis galbana, the culture system includes host computer and lower computer, the host computer is the remote monitoring and control platform based on mobile terminal, is connected with lower computer, is used for real -time monitoring, parameter setting and remote control, the lower computer includes pipeline type light biological reaction circulation unit, control unit, feed addition unit and liquid outlet collection unit, based on this culture system can realize the efficient, stable, low -cost continuous culture of isochrysis galbana.

Provided herein included methods and systems to identify exposure to environmental carcinogens, compounds, and other toxins are disclosed. Additionally provided, include methods and systems to identify risk associated with such environmental carcinogens, compounds, and other toxins. Various embodiments include machine learning models to identify cellular morphometric biomarkers (CMBs). Various methods and systems described herein are capable of using CMBs for diagnostic, prognostic, or forensic purposes. Some embodiments are directed to training a machine learning model for such using CMBs for diagnostic, prognostic, or forensic purposes, including (but not limited to) generating images of biological responses that can be used as a training data set. Additional embodiments are directed to computing devices and / or software that are capable of performing methods as described herein.

This invention provides an organizational attribute analysis system and apparatus that analyze organizational attributes through changes in the biological responses of users participating in online sessions conducted within an organization. [Solution] The tissue attribute analysis device 100 includes a bio-response analysis unit 12 that analyzes changes in the user's bio-response for each online session of multiple organizations in which the same user participates, based on video images obtained of the user during an online session conducted by multiple users, and a tissue attribute analysis unit 13 that analyzes the attributes of the organization based on the changes in bio-response analyzed by the bio-response analysis unit 12 for each online session of multiple organizations. The device analyzes the bio-response of participants in online sessions conducted within an organization and analyzes the attributes of the organization based on the analysis results.

This invention discloses an intelligent biological reaction monitoring system and method, belonging to the field of intelligent monitoring. The system includes: a reaction stage segmentation unit for segmenting and determining multiple reaction stages; a variable relationship mining unit for determining dominant and auxiliary variables, mining linear relationships between variables, coordinating insensitive loss functions, and supervising the training of a soft monitoring module; a continuous monitoring and acquisition unit for configuring monitoring equipment groups, performing continuous monitoring, and determining reaction monitoring data; a data analysis and screening unit for preprocessing data, performing linear analysis and abnormal reaction localization, and determining valid monitoring data; a game equilibrium decision-making unit for performing game equilibrium decisions on abnormal reaction identifiers and determining reaction regulation strategies; and a reaction monitoring management unit for responding to regulation strategies, performing feedback monitoring analysis and reaction monitoring management, improving the accuracy of biological reaction monitoring, achieving real-time online monitoring, and intelligently regulating abnormal situations.

This application discloses a method and apparatus for optimizing a bioreaction process using deep learning, relating to the field of cell bioengineering. The method includes acquiring the first bioreaction type, the first reaction stage, the first basic configuration parameters of the cell bioreactor, and the first monitoring parameters collected by multiple types of sensors in the current bioreaction process within a cell bioreactor; retrieving contextual data related to the first bioreaction type and the first reaction stage from a bioreaction knowledge vector base; constructing prompt words based on the contextual data, the first monitoring parameters, the first bioreaction type, the first reaction stage, the first basic configuration parameters, preset data analysis instructions, and historical question-and-answer data from monitoring and analysis; and inputting the prompt words into a trained large-scale cell bioreaction model, so that the large-scale cell bioreaction model outputs first control parameters for a programmable logic controller to predict the operating state of the cell bioreactor and correct for faults.

This invention relates to an organoid biomechanical response platform and a method for simulating the force exerted on human gait organs. The platform includes an organ-on-a-chip and a biomechanical response device. The biomechanical response device is divided into two types: simulating the force exerted on the organoid during prolonged standing and simulating the force exerted on the organoid during walking or running. The system simulating prolonged standing includes an interconnected pressure chamber base, a one-way valve, a stroke cylinder, a stroke reciprocating structure, and a motor. The system simulating walking or running includes an interconnected pressure chamber base, a stroke cylinder, a stroke reciprocating structure, and a motor. The organoid is placed within the pressure chamber base. The stroke reciprocating structure includes a connecting rod piston, a crankshaft connecting rod, and a crankshaft. The connecting rod portion of the connecting rod piston is connected to the crankshaft via the crankshaft connecting rod. The crankshaft is connected to the motor. The piston portion of the connecting rod piston extends into the stroke cylinder. Compared with existing technologies, this invention achieves stable, repeatable, and quantifiable mechanical stimulation, realistically reproducing human gait stress.

Provided are a compound including a cleavable linker, a use thereof, and an intermediate compound for preparing the same, and more particularly, the compound including a cleavable linker of the present invention may include an active agent (for example, a drug, a toxin, a ligand, a probe for detection, etc.) having a specific function or activity, a —S(═O)(═N—)— functional group which is capable of selectively releasing the active agent, and a functional group which triggers a chemical reaction, a physicochemical reaction and / or a biological reaction by external stimulation, and may further include a ligand (for example, oligopeptide, polypeptide, antibody, etc.) having binding specificity for a desired target receptor.

This invention discloses a bioreactor device, components, and operating method. The device includes: a container forming a sealed containment space, with at least two fluid ports communicating with the containment space; at least one plate, which may be a planar plate or a microporous plate, arranged in parallel within the containment space; and a shaft connected to at least one end of the container, passing through each plate, the plates rotating with the shaft or the container, the shaft being hollow and having openings for conveying fluid within the shaft to each plate. This technical solution is particularly suitable for cell and cell spheroid culture, enabling batch production based on the number and size of cell aggregates, thereby producing highly reproducible, reliable, homogeneous, and uniformly sized products. This improves the controllability, scalability, safety, and compliance of bioreactors, enhances the level of automated production, and meets the needs of large-scale clinical production.

A parallel multi-step bio-reaction system(10) comprising: (a) a substrate arrangement(12) comprising a plurality of bio-reaction substrate holders(18) configured to hold a plurality of bio-reaction substrates(20); (b) a well arrangement(14) comprising a plurality of fluidic wells(22), the fluidic wells(22) corresponding to a plurality of steps of a multi-step bio-reaction; (c) an actuator(16) configured to: (i) move either the substrate arrangement(12) or the well arrangement(14) relative to the other of the substrate arrangement(12) or the well arrangement(14) to change the alignment of the bio-reaction substrates(20) as a group relative to the fluidic wells(22) as a group; and (ii) bring the bio-reaction substrates(20) into and out of contact with fluids in the fluidic wells(22).

Systems, apparatus, articles of manufacture, and methods are disclosed to remotely measure biological response data. An example apparatus includes interface circuitry; machine readable instructions; and programmable circuitry to at least one of instantiate or execute the machine readable instructions to generate a study based on one or more target modalities; transmit the study to electronic devices corresponding to study participants; obtain response data corresponding to the study; and aggregate the response data across the participants and across the modalities.

The invention discloses an automatic in-vitro biological synthesis reaction system which comprises a reaction tank assembly, a mixing assembly, a feeding assembly, a reaction raw material quantity feedback part, a detection part and a control device in communication connection with all the components, and the reaction tank assembly is provided with a tank body used for containing reaction liquid for in-vitro biological reaction; the reaction mixing assembly is used for mixing reaction liquid; the feeding assembly is provided with a feeding pipe communicated with the tank body and a feeding pump communicated with the feeding pipe; the reaction raw material quantity feedback part is used for detecting the raw material quantity in the tank body to obtain raw material quantity parameters; the detection part is used for detecting the reaction liquid and / or the gas generated by the reaction to obtain detection parameters; the control device is provided with a feeding module, an operation module and a mixing module and is used for achieving automatic operation of the steps of automatic feeding, automatic stirring, automatic detection, automatic discharging, automatic cleaning and the like, and the workload and operation knowledge requirements of operators are reduced.

Orthodontic systems and related methods are disclosed for designing and providing tooth moving systems. Methods and orthodontic systems include the generation of an overcorrection in the tooth-receiving cavities of an appliance worn in the dentition. The overcorrection may provide an improved and more accurately applied force or moment applied to a tooth. The overcorrected force or moment can move a tooth closer to a desired position than if not overcorrected as sufficient force can still be applied to the tooth as it gets closer to the desired position. The overcorrected force or moment may also better target the root of the tooth where the biological response to tooth movement occurs. The disclosed method comprises receiving input data comprising an initial tooth arrangement and a target tooth arrangement for a patient's teeth, determining a plurality of treatment stages for repositioning the patient's teeth from the initial tooth arrangement toward the target tooth arrangement, determining, for at least some of the treatment stages, an overcorrected movement corresponding to an overcorrected position for the tooth that is different from the target position specified by the treatment stage, and generating fabrication instructions for a plurality of dental appliances corresponding to the plurality of treatment stages.

The application belongs to the technical field of medicine, and specifically discloses a radiotherapy sensitizer IR808-ATIPA as well as a preparation method and application thereof. The compound is covalently connected by IR808 and ATIPA, has the functions of radiosensitization and CT imaging, and can prolong the retention time in tumors. IR808-ATIPA can enhance X-ray absorption and induce ferroptosis (down-regulate GPX4, up-regulate ACSL4 and ROS) to synergistically improve the effect of radiotherapy. The preparation method comprises the following steps: condensation of phenylhydrazine and 3-methyl-2-butanone to generate an intermediate 1, reaction of the intermediate 1 with 6-bromohexanoic acid to generate an intermediate 2, acyl chloride treatment of the intermediate 2, condensation of the intermediate 2 with triiodoisophthalic acid, and finally reaction and purification of the intermediate 2 with a cyclohexene derivative to obtain the target product. Experiments show that after intratumoral injection of IR808-ATIPA, the micro-CT can guide the radiotherapy target area in real time, and the combination of the micro-CT and low-dose X-ray (4 Gy) can significantly inhibit the growth of tumors and reduce the expression of Ki67. The compound is suitable for tumor treatment with integration of diagnosis and treatment, and has the potential of high biological safety and precise radiotherapy.

Systems and methods can apply machine learning techniques to predict biological responses. One of the methods is performed by at least one processor executing executable logic including at least one machine learning model trained to predict biological responses. The method includes receiving first sequence data of a first molecular sequence, receiving second sequence data of a second molecular sequence, and predicting a biological response for the second molecular sequence based at least partly on the received first and second sequence data.

The invention relates to the technical field of biochemical analysis, and discloses miniaturized biochemical reaction monitoring equipment which comprises a computer case, a shell is fixedly connected to the outer side of the computer case, a bottom plate is arranged in the shell, and a storage vessel is fixedly connected to the top of the bottom plate; the inner top wall of the shell is fixedly connected with a photo-biological reaction lamp, the interior of the shell is fixedly connected with a first supporting plate, the middle of the first supporting plate is fixedly connected with a biosensor, and the side, away from the first supporting plate, of the interior of the computer case is fixedly connected with a second supporting plate; an air cylinder is fixedly connected to the middle of the second supporting plate, a photoelectric detector is fixedly connected to the output end of the air cylinder, and a miniature air conditioner is fixedly connected to the middle of the computer case. Through an automatic linkage mechanism and state vector prediction control, active correction of the reaction process is realized, and the automation degree, the regulation and control precision and the repeatability are improved.

The invention relates to a multi-axis joint simulation biological reaction system and method, and the system comprises a multi-axis mechanical loading module which is used for applying a multi-axis mechanical load to a sample and simulating a muscle-bone mechanical environment; the dynamic degradation module is used for simulating the body fluid environment of the sample by controlling body fluid parameters; the cell co-culture module is used for providing a cell co-culture environment for the sample and simulating an interaction process of the cell and the sample; the monitoring module is used for collecting real-time data of the multi-axis mechanical loading module, the dynamic degradation module and the cell co-culture module and monitoring the sample based on the real-time data. The problem of how to realize mechanical, chemical and biological synchronous simulation and monitoring of instruments in vitro in the prior art is solved.

The application discloses an in-situ biological detection system and method based on an insertion type Raman probe, and the in-situ biological detection system comprises a Raman detection device and a biological detection cabin. The Raman detection device comprises a Raman probe, and the Raman probe is provided with a probe head which is inserted into the biological detection cabin. The biological detection cabin comprises a shell and a biological reaction substrate arranged in the shell, and the shell is provided with a drain hole. After the in-situ biological detection system is assembled, the system is dived to a required position, and the metabolic interaction process between the biological detection cabin and the in-situ environment is monitored in real time and continuously by the Raman detection device. The in-situ biological detection system based on the insertion type Raman probe adopts the in-situ Raman detection method, and has a simpler structure and stable deep-sea in-situ detection performance. Since the system itself is used for in-situ environment detection, the application needs of sensing systems for in-situ detection of various types of organisms can be met, and the system has more sensitive selectivity.

Provided herein included methods and systems to identify exposure to environmental carcinogens, compounds, and other toxins are disclosed. Additionally provided, include methods and systems to identify risk associated with such environmental carcinogens, compounds, and other toxins. Various embodiments include machine learning models to identify cellular morphometric biomarkers (CMBs). Various methods and systems described herein are capable of using CMBs for diagnostic, prognostic, or forensic purposes. Some embodiments are directed to training a machine learning model for such using CMBs for diagnostic, prognostic, or forensic purposes, including (but not limited to) generating images of biological responses that can be used as a training data set. Additional embodiments are directed to computing devices and / or software that are capable of performing methods as described herein.

Provided herein included methods and systems to identify exposure to environmental carcinogens, compounds, and other toxins are disclosed. Additionally provided, include methods and systems to identify risk associated with such environmental carcinogens, compounds, and other toxins. Various embodiments include machine learning models to identify cellular morphometric biomarkers (CMBs). Various methods and systems described herein are capable of using CMBs for diagnostic, prognostic, or forensic purposes. Some embodiments are directed to training a machine learning model for such using CMBs for diagnostic, prognostic, or forensic purposes, including (but not limited to) generating images of biological responses that can be used as a training data set. Additional embodiments are directed to computing devices and / or software that are capable of performing methods as described herein.

Systems and methods can apply machine learning techniques to predict biological responses. One of the methods is performed by at least one processor executing executable logic including at least one machine learning model trained to predict biological responses. The method includes receiving first sequence data of a first molecular sequence, receiving second sequence data of a second molecular sequence, and predicting a biological response for the second molecular sequence based at least partly on the received first and second sequence data.

A method for analyzing a user's reaction to a stimulus is described which includes providing a user stimulus and a stimulation parameter through a sensory stimulator, measuring a biological response and acquiring a biosignal which indicates a neurophysiological reaction provides neurophysiological reaction data which generates sensory metadata indicative of the user's response to the corresponding stimulus. The sensory metadata is archived to obtain a plurality of sensory metadata associated with the user. Each piece of stored sensory metadata is processed according to input information, generating an output information including a reaction index to the stimulus as a function of the input information, and modifying a stimulation parameter according to the reaction index.

The invention discloses a method for site-specific chemical modification of a nano antibody and application of the method, and belongs to the technical field of chemical modification of nano antibodies. The invention aims to provide more modification sites and strategies for the nano antibody. According to the method, on the basis of NHS ester reaction, natural lysine is completely or selectively mutated into arginine by rationally designing an amino acid sequence of a nano antibody, so that accurate control of modification sites is realized. According to one method, after all lysine is mutated, NHS ester only reacts with alpha-amino at the N end of the nano antibody, so that specific modification of the N end is realized; according to the other method, cysteine is introduced to the N end of the nano antibody to react with a 2-cyanobenzothiazole derivative so as to close the N-end alpha-amino group, meanwhile, single lysine is reserved or introduced to serve as a specific modification site, other lysine mutates, and precise functionalization of a nano antibody skeleton or a CDR region is achieved. And the method has wide potential in basic research, imaging and treatment development.

To enable support in the differential diagnosis of heart disease. [Solution] An information processing system comprising: an answer input unit that receives input from a patient's answers to questions for differentiating mental illnesses; a video image acquisition unit that acquires video images of the patient; a biological response detection unit that analyzes the video images to detect changes in the patient's biological responses; and an estimation unit that estimates the mental illness the patient is suffering from by providing the received answers and detected changes in biological responses to a learning model that has learned the answers, changes in biological responses, and mental illnesses.

The invention belongs to the technical field of biological medicines, and relates to a tetrahydropyridine [4, 3-d] pyrimidine derivative as a Hedgehog signaling pathway inhibitor and a preparation method thereof. The tetrahydropyridine [4, 3-d] pyrimidine derivative serving as a Hedgehog signal channel inhibitor is prepared by creatively adjusting the reaction temperature and the ratio of reactants to a catalyst, the reaction yield is greatly increased, the reaction conditions are mild, and the derivative serving as the Hedgehog signal channel inhibitor has good activity on pancreatic cancer cells and medulloblastoma.

The present application relates to the field of bioengineering, and particularly to a production system and method for disposable high-pathogenic microorganism or inactivated vaccine. The method comprises: providing a self-definable assembly bio-culture module and a corresponding management module; the bio-culture module comprises: a bio-reaction device, an intermediate device and a purification device connected through detachable pipelines, and a liquid leakage sensing line is arranged on the pipelines; when the bio-culture module is started, a first set composed of readings on the liquid leakage sensing line is collected; the management module judges the risk state of the bio-culture module through the first set and controls the safe operation thereof. The present application provides a disposable production method and system suitable for high-pathogenic viruses, bacteria and inactivated systems, which has the advantages of flexible assembly, automatic leakage warning, sterilization and the like, and the cumbersome processes (such as centrifugation, cleaning and the like) in the fixed production method are omitted, thereby effectively saving the research and development time and cost.

The application discloses a kind of bioreactor for immune cell culture, comprising: bioreactor tank body and the culture layer being arranged in the inside of bioreactor tank body;Bioreactor tank body top is provided with adjustment liquid inlet, and bioreactor tank body annular face is provided with multiple layers of flushing liquid total inlet, flushing liquid total outlet, supplementary liquid total inlet, supplementary liquid total outlet, and bioreactor tank body bottom is provided with liquid total outlet.The bioreactor for immune cell culture of the application adopts multiple layers of independent culture structure, each layer is equipped with exclusive fluid channel and control system, substantially improves culture area while guaranteeing interlayer environment consistency;Honeycomb plate is designed as regular hexagon honeycomb hole, maximizes the use of culture space, avoids cell aggregation and nutrition blind area, provides uniform growth environment for semi-adherent immune cells, significantly improves cell proliferation efficiency and activity consistency.

The invention discloses a point of care system. The point of care system includes a multichannel bio-reaction device, a surface acoustic wave (SAW) signal reader and a communication module. The multichannel bio-reaction device is configured to receive an analyte collected from a user and generate a plurality of SAW signals each of which corresponds to at least one of a set of target proteomic markers in the analyte. The SAW signal reader is configured to receive the SAW signals and produce a plurality of time-stamped data corresponding to the SAW signals. The communication module configured to receive the plurality of time-stamped data and transmit the received plurality of time-stamped data to a processor, wherein the processor is configured to run a report-generating process for generating a report including an analysis to the set of target proteomic markers.

This invention belongs to the technical field of biological cell reaction equipment, specifically a bioengineering reaction vessel, comprising a vessel body, a pneumatic device, a sensing device, and a regulating device. The bottom of the vessel body is connected to the pneumatic device, and the upper end of the pneumatic device is connected to a main rod. Multiple sensing devices are connected to one side of the main rod, and these sensing devices are arranged vertically and alternately, with a driving connection between the sensing devices and the regulating device. This invention generates uniformly distributed microbubbles through the pneumatic device integrated at the bottom of the vessel body, providing dissolved oxygen and hybrid power to the reaction system. The vertically and alternately arranged sensing devices detect changes in the fluid state at different levels within the vessel in real time due to cell growth, product accumulation, etc., and convert these changes into measurable signals. The regulating device drives the sensing devices to change their states, thereby actively adjusting the mixing intensity and flow field, achieving real-time in-situ sensing and adaptive closed-loop control of the mixing state during the bioreaction process.