Serum-free stem cell culture medium

A culture medium and stem cell technology, applied in the direction of cell culture active agent, culture process, tissue culture, etc., can solve the problems of standardization of cell culture, difficulty of separation and purification of cell culture expression product repeatability, etc. Cell growth and proliferation, the effect of fast expansion

Inactive Publication Date: 2017-03-22
严志海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the process of stem cell culture, a large number of complex proteins in serum bring difficulties to the standardization of cell culture. The complex proteins and multiple cytokines will cause stem cells that are easy to differentiate themselves to produce a variety of completely different cells. Phenotypes, resulting in a variety of completely different cell differentiation trends, also brings great difficulties to the separation and purification of cell culture expression products and the reproducibility of results

Method used

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  • Serum-free stem cell culture medium
  • Serum-free stem cell culture medium
  • Serum-free stem cell culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 A kind of serum-free stem cell culture medium

[0026] A serum-free stem cell medium consisting of the following components and their concentrations: DMEM / F12 1L, insulin 7.5mg / L, transferrin 24.5mg / L, icariin 0.51μM, glycylalanine 765mg / L L, alanyl-L-tyrosine 1.97g / L, polylysine 0.34mg / L, vitamin E 0.32mg / L, polyvinylpyrrolidone 0.042mg / L, transforming growth factor-β 19μg / L, reduced Glutathione 0.43mg / L, sodium bicarbonate 0.52g / L.

[0027] The culture medium preparation method is:

[0028] (1) Accurately weigh the corresponding concentrations of insulin, transferrin, icariin, glycylalanine, alanyl-L-tyrosine, polylysine, vitamin E, polyvinylpyrrolidone, Transforming growth factor-β, reduced glutathione, dissolved in ultrapure water at a temperature of 25°C, stirred until completely dissolved, to obtain solution I;

[0029] (2) Add sodium bicarbonate of corresponding concentration to the above solution I, stir well, add 1L DMEM / F12 culture medium, stir...

Embodiment 2

[0032] Embodiment 2 A kind of serum-free stem cell culture medium

[0033] A serum-free stem cell medium consisting of the following components and their concentrations: DMEM-L 1L, insulin 6mg / L, transferrin 21mg / L, icariin 0.45μM, glycylalanine 365mg / L, Alanyl-L-tyrosine 1.85g / L, polylysine 0.15mg / L, vitamin E 0.3mg / L, polyvinylpyrrolidone 0.03mg / L, transforming growth factor-β 10μg / L, reduced gluten Glutathione 0.025mg / L, sodium bicarbonate 0.45g / L.

[0034] The preparation method is similar to Example 1.

Embodiment 3

[0035] Embodiment 3 A kind of serum-free stem cell culture medium

[0036] A serum-free stem cell medium consisting of the following components and their concentrations: DMEM-L 1L, insulin 15mg / L, transferrin 35mg / L, icariin 0.55μM, glycylalanine 850mg / L, Alanyl-L-tyrosine 2.15g / L, polylysine 0.75mg / L, vitamin E 0.5mg / L, polyvinylpyrrolidone 0.045mg / L, transforming growth factor-β 30μg / L, reduced gluten Glutathione 0.54mg / L, sodium bicarbonate 0.65g / L.

[0037] The preparation method is similar to Example 1.

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Abstract

The invention belongs to the technical field of cell culture media and particularly relates to a serum-free stem cell culture medium. The serum-free stem cell culture medium comprises a basic culture medium, insulin, transferrin, icariin, glycylalanine, alanyl-L-tyrosine, polylysine, vitamin E, polyvinylpyrrolidone, transforming growth factor-beta, reductive glutathione and sodium bicarbonate. The serum-free stem cell culture medium can promote cell growth and proliferation and can remarkably promote stem cell differentiation and culture the proliferated stem cells to protect a cell retrogression model.

Description

technical field [0001] The invention belongs to the technical field of cell culture medium, in particular to a serum-free stem cell culture medium. Background technique [0002] Many studies have shown that stem cells can not only self-renew, but also differentiate into other functional cells under suitable conditions. Therefore, stem cells are expected to become an effective means of treating difficult human diseases. However, the content of stem cells in normal adult tissues is very small. How to rapidly expand and cultivate stem cells in vitro is an important technology for studying the mechanism of stem cells and exploring their therapeutic methods in the treatment of human diseases. [0003] A patent application (CN103243070A) discloses a stem cell culture medium and its application. The culture medium components include basal medium, fetal bovine serum, cytokines or protein polypeptides, vitamins and lipids. The medium provided by it can rapidly expand stem cells with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0667C12N5/0663C12N5/0665C12N2500/25C12N2500/30C12N2500/38C12N2500/50C12N2500/90C12N2501/15
Inventor 严志海
Owner 严志海
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