Serum-free stem cell culture medium

A culture medium and stem cell technology, applied in the direction of cell culture active agent, culture process, tissue culture, etc., can solve the problems of standardization of cell culture, difficulty of separation and purification of cell culture expression product repeatability, etc. Cell growth and proliferation, the effect of fast expansion
CN106520690AInactive Publication Date: 2017-03-22严志海
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
严志海
Publication Date
2017-03-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of cell culture media and particularly relates to a serum-free stem cell culture medium. The serum-free stem cell culture medium comprises a basic culture medium, insulin, transferrin, icariin, glycylalanine, alanyl-L-tyrosine, polylysine, vitamin E, polyvinylpyrrolidone, transforming growth factor-beta, reductive glutathione and sodium bicarbonate. The serum-free stem cell culture medium can promote cell growth and proliferation and can remarkably promote stem cell differentiation and culture the proliferated stem cells to protect a cell retrogression model.
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Description

technical field

[0001] The invention belongs to the technical field of cell culture medium, in particular to a serum-free stem cell culture medium. Background technique

[0002] Many studies have shown that stem cells can not only self-renew, but also differentiate into other functional cells under suitable conditions. Therefore, stem cells are expected to become an effective means of treating difficult human diseases. However, the content of stem cells in normal adult tissues is very small. How to rapidly expand and cultivate stem cells in vitro is an important technology for studying the mechanism of stem cells and exploring their therapeutic methods in the treatment of human diseases.

[0003] A patent application (CN103243070A) discloses a stem cell culture medium and its application. The culture medium components include basal medium, fetal bovine serum, cytokines or protein polypeptides, vitamins and lipids. The medium provided by it can rapidly expand stem cells with...

Claims

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