A double-cell continuous fermentation method with high ethanol production

Abstract

The invention discloses a double-cell continuous fermentation method with high ethanol production. Firstly, a seed medium is used to cultivate bacillus amyloliquefaciens and chlorella to ferment seeds, and finally, a double-cell co-cultivation fermentation medium is used to ferment and produce ethanol. During the seed culture process of this process, Bacillus amyloliquefaciens and Chlorella grow rapidly and the final cell concentration is high. During the fermentation process of this process, the composition of the fermentation medium is simple, and there is no need to add trace elements such as carbon sources, vitamins, and amino acids. transform.

Description

technical field [0001] The invention belongs to the field of cell fermentation engineering, and in particular relates to a high-yield double-cell continuous fermentation method. Background technique [0002] Biomass ethanol is a low-emission, green, and environmentally friendly clean energy. Substituting or partially replacing gasoline with ethanol as future vehicle fuel can greatly alleviate the energy crisis. At the same time, from the perspective of the global carbon cycle, the carbon dioxide released after ethanol combustion is re-fixed by green plants, which can slow down the greenhouse effect, so it has attracted more and more attention from governments and scientists. [0003] The production of biomass ethanol requires the use of renewable biomass resources. At present, the method of lignocellulose hydrolysis and microbial fermentation is mainly used in the world. Most of the cellulase used in this method is imported, and the cost is high. The fermentation strain yeas...

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Problems solved by technology

[0003] The production of biomass ethanol requires the use of renewable biomass resources. At present, the method of lignocellulose hydrolysis and microbial fermentation is mainly used in the world. Most of the cellulase used in this method is imported, and the cost is high. The fermentation strain yeast cannot withstand high concentration Ethanol and poor tolerance to inhibitors in lignocellulosic hydrolysates

In addition, there is also a method of synchronous saccharification and fermentation, but no strain suitable for large-scale production has been found. The artificially constructed yeast genetically engineered bacteria have low enzymatic hydrolysis efficiency and poor stability, and the ethanol fermentation level of other screened wild strains cannot reach industrialization. need

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