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A Mutagenic Strain H8 of Bifidobacterium longum

A technology of Bifidobacterium longum and mutagenic strains, which is applied to bacteria, mutant preparation, microorganisms and other directions, can solve the problem of Bifidobacterium longum being intolerant to oxygen and other problems, and achieve good growth and oxygen tolerance, increase growth rate, and delay growth. period shortening effect

Active Publication Date: 2022-04-26
NANJING SHENGDE INST OF BIOTECHNOLOGY CO LTD +1
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Problems solved by technology

[0004] In order to solve the problem that the existing Bifidobacterium longum is not resistant to oxygen, the present invention provides a mutagenic oxygen-tolerant Bifidobacterium longum strain H8, which has a short lag period and a high growth rate

Method used

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  • A Mutagenic Strain H8 of Bifidobacterium longum
  • A Mutagenic Strain H8 of Bifidobacterium longum
  • A Mutagenic Strain H8 of Bifidobacterium longum

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Embodiment Construction

[0020] The present invention will be described in further detail below in conjunction with the examples. Unless otherwise specified, the reagents and consumables used in the present invention are commercially available conventional products.

[0021] 1. The cultivation and growth curve determination of the original strain 6189 of Bifidobacterium longum

[0022] Streak the original strain of Bifidobacterium longum 6189 on a plate containing solid medium, culture it anaerobically at 37°C for 48 hours, pick a single colony after the strain has obvious single colonies, and inoculate it into the liquid medium with 1% inoculum amount, 37 ℃ anaerobic culture, sampling every 2 hours to measure the growth, and draw the growth curve, such as figure 1 As shown, the strain is in the lag phase in the early stage of growth, and grows slowly until the strain reaches the plateau phase.

[0023] The above solid medium components: 10 g of meat juice, 5 g of peptone, 3 g of yeast powder, 5 g of...

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Abstract

A Bifidobacterium longum mutagenic strain H8, which is named Bifidobacterium longum taxonomically, and was deposited in the General Microbiology Center of China Committee for Microbial Culture Collection on September 5, 2019, with a deposit number It is CGMCC No.18468. The bifidobacterium longum mutagenic strain H8 of the present invention is obtained by using the bifidobacterium longum 6189 as a starting strain and screening by plasma mutagenesis treatment technology. Compared with the starting strain, the mutagenic strain H8 of the present invention has a lag period greatly shortened, and a growth rate is also significantly increased.

Description

technical field [0001] The present invention relates to microbial preparations, more specifically, it relates to an oxygen-tolerant Bifidobacterium longum mutagenic strain H8. Background technique [0002] Bifidobacterium longum, a kind of probiotics, has the effect of regulating the human intestinal tract and improving health. It is taken from the healthy human intestinal tract and belongs to the genus Bifidobacterium. Bifidobacterium (Bifidobacterium) is an anaerobic Gram-positive bacillus isolated from the feces of healthy breast-fed infants by French scholar Tissier in 1899. The ends are often forked, hence the name Bifidobacterium. Bifidobacterium is a classic probiotic, and its promoting effect on human health has been proved by extensive research. Bifidobacterium, Bifidobacterium longum. Bifidobacteria can produce lactic acid and acetic acid after fermentation in the human intestine, which can improve the utilization rate of calcium, phosphorus and iron, and can tre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N13/00C12N15/01C12R1/01
CPCC12N13/00C12N15/01
Inventor 史鲁秋汪昌国薛虹宇苏桂珍其他发明人请求不公开姓名
Owner NANJING SHENGDE INST OF BIOTECHNOLOGY CO LTD
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