Bifidobacterium longum mutant strain H8

A technology of Bifidobacterium longum and mutagenic strains, which is applied in the direction of bacteria, mutant preparation, treatment of microorganisms with electricity/wave energy, etc., and can solve the problems of Bifidobacterium longum intolerance to oxygen, etc.

Active Publication Date: 2021-04-09
NANJING SHENGDE INST OF BIOTECHNOLOGY CO LTD +1
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  • Claims
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Problems solved by technology

[0004] In order to solve the problem that the existing Bifidobacterium longum is not resistant to oxygen, the present invention provides a mutagenic oxygen-tolerant Bifidobacterium longum strain H8, which has a short lag period and a high growth rate

Method used

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  • Bifidobacterium longum mutant strain H8
  • Bifidobacterium longum mutant strain H8
  • Bifidobacterium longum mutant strain H8

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Embodiment Construction

[0020] The present invention will be described in further detail below in conjunction with the examples. Unless otherwise specified, the reagents and consumables used in the present invention are commercially available conventional products.

[0021] 1. The cultivation and growth curve determination of the original strain 6189 of Bifidobacterium longum

[0022] Streak the original strain 6189 of Bifidobacterium longum on a plate containing solid medium, culture it anaerobically at 37°C for 48 h, pick a single colony after the strain has obvious single colonies, and inoculate it into the liquid medium with 1% inoculum. Anaerobic culture at 37°C, sampling every 2 hours to measure the growth, and drawing the growth curve, such as figure 1 As shown, the strain is in the lag phase in the early stage of growth, and grows slowly until the strain reaches the plateau phase.

[0023] The above solid medium components: 10 g of meat juice, 5 g of peptone, 3 g of yeast powder, 5 g of D(+)...

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Abstract

The invention relates to a bifidobacterium longum mutant strain H8. The bifidobacterium longum mutant strain H8 is named as Bifidobacterium longum longum in taxonomy, and is collected in China General Microbiological Culture Collection Center on September 05, 2019, and the collection number is CGMCC No. 18468. The bifidobacterium longum mutant strain H8 according to the invention is obtained by taking bifidobacterium longum 6189 as an original strain and adopting a plasma mutagenesis treatment technology for screening. Compared with the original strain, the mutant strain H8 according to the invention has the advantages that the lag phase is greatly shortened, and the growth rate is also remarkably increased.

Description

technical field [0001] The present invention relates to microbial preparations, more specifically, it relates to an oxygen-tolerant Bifidobacterium longum mutagenic strain H8. Background technique [0002] Bifidobacterium longum, a kind of probiotics, has the effect of regulating the human intestinal tract and improving health. It is taken from the healthy human intestinal tract and belongs to the genus Bifidobacterium. Bifidobacterium (Bifidobacterium) is an anaerobic Gram-positive bacillus isolated from the feces of healthy breast-fed infants by French scholar Tissier in 1899. The ends are often forked, hence the name Bifidobacterium. Bifidobacterium is a classic probiotic, and its promoting effect on human health has been proved by extensive research. Bifidobacterium, Bifidobacterium longum. Bifidobacteria can produce lactic acid and acetic acid after fermentation in the human intestine, which can improve the utilization rate of calcium, phosphorus and iron, and can tre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N13/00C12N15/01C12R1/01
CPCC12N13/00C12N15/01
Inventor 史鲁秋汪昌国薛虹宇苏桂珍其他发明人请求不公开姓名
Owner NANJING SHENGDE INST OF BIOTECHNOLOGY CO LTD
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