Method for promoting sphalerite bioleaching under high altitude and low oxygen environment by Rhodiola rosea

A sphalerite and bioleaching technology, applied in the direction of improving process efficiency, can solve the problems of low oxygen content, ultraviolet intensity, cell energy metabolism disorder, and long lag period of microorganisms, so as to enhance the adaptability of strains and enhance oxygen The effect of using abilities and shortening the growth lag period

Active Publication Date: 2022-03-18
SOUTHWEAT UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the existing technologies are all aimed at promoting bioleaching methods in ordinary environments, and there is no precedent for promoting bioleaching methods in high-altitude and low-oxygen environments. High-altitude areas have low oxygen content and high ultraviolet intensity, causing microorganisms to leaching Lag period too long
[0005] At present, many studies have shown that under low oxygen and low pressure environment, it will lead to increased oxidative stress, lipid peroxidation, difficulty in respiratory metabolism, and inhibition of ATP production, leading to disturbance of cellular energy metabolism
Excessive UV intensity causes an increase in ROS in the bioleaching system, and ROS can cause oxidative damage to proteins, lipids and nucleic acids, resulting in a decrease in microbial activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for rhodiola to promote the bioleaching of sphalerite in a high-altitude low-oxygen environment, comprising: in a high-altitude mining area, the activated acidophilic Thiobacillus ferrooxidans liquid (acidophilic Thiobacillus ferrooxidans liquid active The number of bacteria is 1×10 6 indivual. ) into the culture medium, then add rhodiola rosea extract, ore pulp, and culture with constant temperature and vibration.

[0024] To further optimize the technical scheme: the volume ratio of acidophilic Thiobacillus ferrooxidans liquid to medium is 1:9.

[0025] For further optimizing the technical scheme: the medium is a 4.5K medium, and the formula is: (NH 4 ) 2 SO 4 1.0g / L, KCl0.05g / L, K 2 HPO 4 0.05 g / L, MgSO 4 ·7H 2 O 0.05g / L, Ca(NO 3 ) 2 0.001g / L, FeSO 4 ·7H 2 O 15.0g / L (adjust the pH value with 10% (v / v) dilute sulfuric acid, and control the pH value of the medium at 1.5-3.5).

[0026] To further optimize the technical scheme: the final concentrati...

Embodiment 2

[0030] A method for rhodiola to promote the bioleaching of sphalerite in a high-altitude low-oxygen environment, comprising: in a high-altitude mining area, the activated acidophilic Thiobacillus ferrooxidans liquid (acidophilic Thiobacillus ferrooxidans liquid active The number of bacteria is 5×10 6 indivual. ) into the culture medium, then add rhodiola rosea extract, ore pulp, and culture with constant temperature and vibration.

[0031] To further optimize the technical scheme: the volume ratio of acidophilic Thiobacillus ferrooxidans liquid to medium is 1:9.

[0032] For further optimizing the technical scheme: the medium is a 4.5K medium, and the formula is: (NH 4 ) 2 SO 4 3.0g / L, KCl0.55g / L, K 2 HPO 4 0.25 g / L, MgSO 4 ·7H 2 O 0.25g / L, Ca(NO 3 ) 2 0.015g / L, FeSO 4 ·7H 2 O 25.0g / L.

[0033] To further optimize the technical scheme: the final concentration of Rhodiola rosea extract is 0.5 μg / L.

[0034] In order to further optimize the technical scheme: the fi...

Embodiment 3

[0037] A method for rhodiola to promote the bioleaching of sphalerite in a high-altitude low-oxygen environment, comprising: in a high-altitude mining area, the activated acidophilic Thiobacillus ferrooxidans liquid (acidophilic Thiobacillus ferrooxidans liquid active The number of bacteria is 9×10 6 indivual. ) into the culture medium, then add rhodiola rosea extract, ore pulp, and culture with constant temperature and vibration.

[0038] To further optimize the technical scheme: the volume ratio of acidophilic Thiobacillus ferrooxidans liquid to medium is 1:9.

[0039] For further optimizing the technical scheme: the medium is a 4.5K medium, and the formula is: (NH 4 ) 2 SO 4 5.0g / L, KCl1.0g / L, K 2 HPO 4 0.50 g / L, MgSO 4 ·7H 2 O 0.50g / L, Ca(NO 3 ) 2 0.02g / L, FeSO 4 ·7H 2 O 35.0g / L.

[0040] To further optimize the technical scheme: the final concentration of Rhodiola rosea extract was 1 μg / L.

[0041] In order to further optimize the technical scheme: the final...

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PUM

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Abstract

The invention discloses a method for Rhodiola rosea to promote the bioleaching of sphalerite in a high-altitude and low-oxygen environment. It belongs to the technical field of biometallurgy. Including: In the high-altitude mining area, the acidophilic Thiobacillus ferrooxidans is inoculated into the medium, and the extract of Rhodiola rosea and the pulp are added, and the culture is shaken at a constant temperature after mixing. The invention is simple to operate, and the rhodiola rosea extract used is harmless and non-toxic, and the low-grade sulfide ore waste rock containing copper, zinc, nickel, etc. For tailings, compared with ordinary ore leaching microorganisms, the bacterial stagnation period is shortened by 60% to 80%, and the leaching rate of valuable metals is increased by 2% to 10%, which can significantly improve the ore microbial leaching efficiency in high-altitude low-oxygen environments.

Description

technical field [0001] The invention relates to the technical field of biometallurgy, in particular to a method for rhodiola to promote the bioleaching of sphalerite in a high-altitude and low-oxygen environment. Background technique [0002] With the gradual development of mineral resources, most of the copper resources that have been proven at home and abroad but are yet to be developed are distributed in remote areas, such as the Yulong Copper Mine in my country, and the Qulong Copper Mine in Tibet, which is under investigation, is known as the largest copper mine in Asia. The common characteristics of these areas are harsh natural conditions, fragile ecological environment, remote location, and backward infrastructure and industrial supporting facilities. In the process of developing this type of mine, traditional technologies such as pyrometallurgy face severe challenges in many aspects such as production cost and environmental protection. [0003] Compared with tradit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C22B3/18C22B19/20C22B15/00C22B23/00
CPCC22B3/18C22B19/20C22B15/0065C22B23/0407Y02P10/20
Inventor 傅开彬候普尧田莉查威白贵琪龙美樵周艾莉邓全淋
Owner SOUTHWEAT UNIV OF SCI & TECH
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