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Method for promoting bioleaching of marmatite in high-altitude low-oxygen environment by acetazolamide

A technology of acetazolamide and sphalerite, applied in the direction of improving process efficiency, etc., can solve problems such as low oxygen content, ultraviolet intensity, disordered cell energy metabolism, and reduced microbial activity, so as to enhance the adaptability of bacteria and enhance oxygen The effect of using abilities and shortening the growth lag period

Active Publication Date: 2021-10-01
SOUTHWEAT UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing technologies are all aimed at promoting bioleaching methods in ordinary environments, and there is no precedent for promoting bioleaching methods in high-altitude and low-oxygen environments. High-altitude areas have low oxygen content and high ultraviolet intensity, causing microorganisms to leaching Lag period too long
[0005] At present, many studies have shown that under low oxygen and low pressure environment, it will lead to increased oxidative stress, lipid peroxidation, difficulty in respiratory metabolism, and inhibition of ATP production, leading to disturbance of cellular energy metabolism
Excessive UV intensity causes an increase in ROS in the bioleaching system, and ROS can cause oxidative damage to proteins, lipids and nucleic acids, resulting in a decrease in microbial activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for acetazolamide to promote the bioleaching of sphalerite in a high-altitude low-oxygen environment, comprising: in a high-altitude mining area, the activated acidophilic Thiobacillus ferrooxidans liquid (the number of viable bacteria is 1 × 10 6 1) inoculated into the culture medium, then added acetazolamide and ore pulp, and cultured with constant temperature shaking.

[0025] To further optimize the technical scheme: the volume ratio of acidophilic Thiobacillus ferrooxidans liquid to medium is 1:9.

[0026] For further optimizing the technical scheme: the medium is a 4.5K medium, and the formula is: (NH 4 ) 2 SO 4 1.0g / L, KCl0.05g / L, K 2 HPO 4 0.05 g / L, MgSO 4 ·7H 2 O 0.05g / L, Ca(NO 3 ) 2 0.001g / L, FeSO 4 ·7H 2 O 15.0g / L (adjust the pH value with 10% (v / v) dilute sulfuric acid, and control the pH value of the medium at 1.5-3.5).

[0027] To further optimize the technical scheme: the final concentration of acetazolamide is 0.1mg / L.

[0028] In or...

Embodiment 2

[0031] A method for acetazolamide to promote the bioleaching of sphalerite in a high-altitude low-oxygen environment, comprising: in a high-altitude mining area, the activated acidophilic Thiobacillus ferrooxidans liquid (the number of viable bacteria is 4 × 10 6 1) inoculated into the culture medium, then added acetazolamide and ore pulp, and cultured with constant temperature shaking.

[0032] To further optimize the technical scheme: the volume ratio of acidophilic Thiobacillus ferrooxidans liquid to medium is 1:9.

[0033] For further optimizing the technical scheme: the medium is a 4.5K medium, and the formula is: (NH 4 ) 2 SO 4 3.0g / L, KCl0.55g / L, K 2 HPO 4 0.25 g / L, MgSO 4 ·7H 2 O 0.25g / L, Ca(NO 3 ) 2 0.015g / L, FeSO 4 ·7H 2 O 25.0g / L.

[0034] To further optimize the technical scheme: the final concentration of acetazolamide is 5mg / L.

[0035] In order to further optimize the technical scheme: the final concentration of the pulp is 50g / L.

[0036] In order ...

Embodiment 3

[0038] A method for acetazolamide to promote the bioleaching of sphalerite in a high-altitude low-oxygen environment, comprising: inoculating the activated acidophilic Thiobacillus ferrooxidans solution into the culture medium in a high-altitude mining area, and then adding acetazolamide , pulp, constant temperature shock culture.

[0039] To further optimize the technical scheme: acidophilic Thiobacillus ferrooxidans liquid (the number of viable bacteria is 9×10 6 ) to medium volume ratio of 1:9.

[0040] For further optimizing the technical scheme: the medium is a 4.5K medium, and the formula is: (NH 4 ) 2 SO 4 5.0g / L, KCl1.0g / L, K 2 HPO 4 0.50 g / L, MgSO 4 ·7H 2 O 0.50g / L, Ca(NO 3 ) 2 0.02g / L, FeSO 4 ·7H 2 O 35.0g / L.

[0041] To further optimize the technical scheme: the final concentration of acetazolamide is 10mg / L.

[0042] In order to further optimize the technical scheme: the final concentration of the pulp is 200g / L.

[0043] In order to further optimize ...

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Abstract

The invention discloses a method for promoting bioleaching of marmatite in a high-altitude low-oxygen environment by acetazolamide, and belongs to the technical field of biological metallurgy. The method for promoting bioleaching of the marmatite in the high-altitude low-oxygen environment by the acetazolamide comprises the following steps of inoculating acidophilic thiobacillus ferrooxidans into a culture medium, adding the acetazolamide and ore pulp, and carrying out constant-temperature shaking culture in a high-altitude mining area. The method for promoting bioleaching of the marmatite in the high-altitude low-oxygen environment by the acetazolamide is easy to operate, the used acetazolamide is harmless and non-toxic, and the acetazolamide is added in the biological leaching process to leach low-grade sulfide ore waste rocks or tailings containing copper, zinc, nickel and other metals in the high-altitude mining area; compared with common ore leaching microorganisms, the bacterial stagnation period is shortened by 60%-80%, the leaching rate of valuable metals is increased by 3%-10%, and the ore microbiological leaching efficiency in the high-altitude low-oxygen environment can be remarkably improved.

Description

technical field [0001] The invention relates to the technical field of biometallurgy, in particular to a method for acetazolamide to promote the bioleaching of sphalerite in a high-altitude and low-oxygen environment. Background technique [0002] With the gradual development of mineral resources, most of the copper resources that have been proven at home and abroad but are yet to be developed are distributed in remote areas, such as the Yulong Copper Mine in my country, and the Qulong Copper Mine in Tibet, which is under investigation, is known as the largest copper mine in Asia. The common characteristics of these areas are harsh natural conditions, fragile ecological environment, remote location, and backward infrastructure and industrial supporting facilities. In the process of developing this type of mine, traditional technologies such as pyrometallurgy face severe challenges in many aspects such as production cost and environmental protection. [0003] Compared with t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C22B3/18C22B19/20
CPCC22B3/18C22B19/20Y02P10/20
Inventor 傅开彬候普尧田莉查威白贵琪龙美樵张家铭邓全淋
Owner SOUTHWEAT UNIV OF SCI & TECH
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