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Method and detection device for rapidly detecting viral nucleic acid

A virus nucleic acid and detection device technology, applied in the field of virus detection, can solve the problems of difficult on-site rapid detection, low viral load, and high environmental requirements for nucleic acid detection methods

Pending Publication Date: 2021-07-30
青岛浩铂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] The recent recurrence of new coronary pneumonia in China is caused by imported goods carrying the new crown virus and then infecting contacts. Moreover, due to the low viral load in cold chain fresh food or related environments, routine sampling and detection methods There is a risk of missed detection, and conventional nucleic acid detection methods (such as PCR method) have defects such as high environmental requirements, long detection time, difficult operation, and difficulty in rapid on-site detection

Method used

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  • Method and detection device for rapidly detecting viral nucleic acid
  • Method and detection device for rapidly detecting viral nucleic acid
  • Method and detection device for rapidly detecting viral nucleic acid

Examples

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Comparison scheme
Effect test

Embodiment 1

[0116] This embodiment provides a method for rapid detection of new coronavirus nucleic acid, which specifically includes the following steps:

[0117] (1) The sampling diaphragm is wiped and sampled on the surface of the sample to be tested;

[0118] (2) Place the sampling membrane after sampling in the sample lysis chamber 2 equipped with lysate and carry out lysis treatment for 5 minutes to obtain a nucleic acid solution, wherein the lysate contains proteinase K 20mg / L, Tween 20 0.1%, urea 2 % and TritonX-1000.1%;

[0119] (3) The above-mentioned nucleic acid solution is transported to the first detection and color development chamber 5 and the second detection and color development chamber 6 equipped with reaction reagents respectively through the delivery device 3 to carry out nucleic acid detection and color development reaction, and the reaction time is 30min. The reaction is carried out at 60°C (it can be placed in a 60°C incubator or a water bath), wherein the reacti...

Embodiment 2

[0122] The present embodiment provides a method for rapid detection of norovirus nucleic acid, which specifically includes the following steps:

[0123] (1) The sampling diaphragm is wiped and sampled on the surface of the sample to be tested;

[0124] (2) Place the sampling membrane after sampling in the sample lysis chamber 2 equipped with lysate and carry out lysis treatment for 5 minutes to obtain a nucleic acid solution, wherein the lysate contains proteinase K 20mg / L, Tween 20 0.1%, urea 2 % and TritonX-1000.1%;

[0125] (3) The above-mentioned nucleic acid solution is transported to the first detection and color development chamber 5 and the second detection and color development chamber 6 equipped with reaction reagents respectively through the delivery device 3 to carry out nucleic acid detection and color development reaction, and the reaction time is 30min. The reaction is carried out at 60°C, wherein the reaction reagents in the first detection and color developme...

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Abstract

The invention provides a method and a detection method for rapidly detecting viral nucleic acid, belongs to the technical field of virus detection, and can solve the technical problems that a conventional nucleic acid detection method is high in environment requirement, relatively long in detection time, high in operation difficulty, difficult to realize on-site rapid detection and the like. The detection method comprises the steps of wiping and sampling, sample cracking treatment, detection, color development treatment and the like. The detection device comprises a base body, a sample cracking cavity, a conveying device and a detection and color development cavity. The method and the device have the characteristics of simplicity and convenience in operation, high sensitivity, short detection time, capability of realizing on-site quick detection and the like. The method and the device can be applied to the field rapid detection of viral nucleic acid.

Description

technical field [0001] The invention belongs to the technical field of virus detection, and in particular relates to a method for rapidly detecting viral nucleic acid and a detection device thereof. Background technique [0002] The recent recurrence of new coronary pneumonia in China is caused by imported goods carrying the new crown virus and then infecting contacts. Moreover, due to the low viral load in cold chain fresh food or related environments, routine sampling and detection methods There is a risk of missed detection, and conventional nucleic acid detection methods (such as PCR method) have defects such as high environmental requirements, long detection time, difficult operation, and difficulty in realizing on-site rapid detection. [0003] However, my country needs to import a large amount of cold chain commodities and raw materials every day. For institutions such as import and export ports and food production enterprises, there is an urgent need to develop a vir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6844C12N15/11C12M1/34
CPCC12Q1/6844C12Q1/701C12Q2531/119C12Q2561/113C12Q2563/107Y02A50/30
Inventor 周玭刘金燕温云飞王成林姜文彬赵美青
Owner 青岛浩铂生物科技有限公司
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