CHO-K1 suspension acclimatization culture medium and acclimatization method

A CHO-K1, suspension culture technology, applied in biochemical equipment and methods, culture process, tissue culture, etc., can solve the problems of long doubling time, easy to agglomerate, low viability, etc., to achieve fast amplification speed, cell The effect of non-agglomeration activity rate and short cell culture time

Active Publication Date: 2019-02-12
SHANGHAI OPM BIOSCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned shortcoming of the prior art, the object of the present invention is to provide a kind of CHO-K1 suspension domestication culture medi

Method used

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  • CHO-K1 suspension acclimatization culture medium and acclimatization method
  • CHO-K1 suspension acclimatization culture medium and acclimatization method
  • CHO-K1 suspension acclimatization culture medium and acclimatization method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. Materials and methods

[0045] 1.1 Experimental reagents and cell lines

[0046] product name

brand

Item No.

level

CHO-K1

ATCC

CCL-61

NA

CD CHO Medium

Gibco

10743

cell culture grade

recombinant expression of IGF-1

Primer Genen

105-03

cell culture grade

Poloxamer 188

Sigma

P2164009

Analytical pure

FBS

Gibco

10099

NA

L-glutamine

Gibco

25030

cell culture grade

F-12K

ATCC

30-2004

cell culture grade

[0047] 1.2 Main instruments and equipment

[0048]

[0049] 1.4 Experimental method

[0050] 1.4.1 Phase I: Adherence to lower serum

[0051] CHO-K1 cells were cultured in F-12K medium containing 10% FBS. When the confluence of the cells reached 70-80%, they were digested with trypsin, mixed slowly by pipetting, and mixed with 0.4×10 5 The density of cells / ml was inoculated into 10ml F-12K medium containing 10% FBS for...

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PUM

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Abstract

The invention provides a CHO-K1 suspension acclimatization culture medium and an acclimatization method thereof. The culture medium at least comprises CD CHO Medium, IGF-1, poloxamer 188 and conditionmedium. When the culture medium is adopted to acclimatize CHO-K1 cells, the cell culture time is short (less than 2 months), the multiplication speed is high, the cells cannot be clustered, and the survival rate is high.

Description

technical field [0001] The invention relates to a CHO-K1 suspension domestication medium and a domestication method, belonging to the field of biotechnology. Background technique [0002] CHO, which was isolated from an adult female hamster ovary by Dr. Theodore T. Puck of the University of Colorado in 1957, is an epithelial adherent cell and is a cell line widely used in bioengineering. The cells are immortal and can be passed down for more than a hundred generations, and are widely used in bioengineering. In addition, CHO cells have another advantage in the use of genetic engineering. This cell belongs to fibroblast and is a non-secreting cell. It rarely secretes CHO endogenous proteins, so it is very beneficial for the separation and purification of target proteins. . It can form active dimers (such as interleukin 2) and has the function of glycosylation (such as EPO). CHO is an ideal host for expressing complex biological macromolecules. [0003] CHO-K1 cells are most...

Claims

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Application Information

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IPC IPC(8): C12N5/077
CPCC12N5/0656C12N2500/30C12N2500/90C12N2501/105
Inventor 肖志华梁欠欠
Owner SHANGHAI OPM BIOSCI CO LTD
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