CHO-K1 suspension acclimatization culture medium and acclimatization method

Abstract

The invention provides a CHO-K1 suspension acclimatization culture medium and an acclimatization method thereof. The culture medium at least comprises CD CHO Medium, IGF-1, poloxamer 188 and conditionmedium. When the culture medium is adopted to acclimatize CHO-K1 cells, the cell culture time is short (less than 2 months), the multiplication speed is high, the cells cannot be clustered, and the survival rate is high.

Description

technical field [0001] The invention relates to a CHO-K1 suspension domestication medium and a domestication method, belonging to the field of biotechnology. Background technique [0002] CHO, which was isolated from an adult female hamster ovary by Dr. Theodore T. Puck of the University of Colorado in 1957, is an epithelial adherent cell and is a cell line widely used in bioengineering. The cells are immortal and can be passed down for more than a hundred generations, and are widely used in bioengineering. In addition, CHO cells have another advantage in the use of genetic engineering. This cell belongs to fibroblast and is a non-secreting cell. It rarely secretes CHO endogenous proteins, so it is very beneficial for the separation and purification of target proteins. . It can form active dimers (such as interleukin 2) and has the function of glycosylation (such as EPO). CHO is an ideal host for expressing complex biological macromolecules. [0003] CHO-K1 cells are most...

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Problems solved by technology

[0006] In view of the above-mentioned shortcoming of the prior art, the object of the present invention is to provide a kind of CHO-K1 suspension domestication culture medium and domestication method, be used to solve the CHO-K1 culture time long, easy agglomeration, low activity rate in the prior art. , and the problem of long doubling time

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