Method for producing siniperca chuatsi infectious spleen and kidney necrosis virus and siniperca chuatsi rhabdovirus through microcarrier suspension culturing CPB cells

A spleen and kidney necrosis virus and microcarrier culture technology, applied in the field of cell engineering, can solve the problems of high cost, high titer virus technical difficulties, long time, etc., and achieve the effects of low production cost, low pollution probability and small labor force.
CN107326016AActive Publication Date: 2017-11-07PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
Publication Date
2017-11-07

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Abstract

The invention discloses a method for producing a siniperca chuatsi infectious spleen and kidney necrosis virus and a siniperca chuatsi rhabdovirus through microcarrier suspension culturing CPB cells. The method comprises the steps of selecting the CPB cells as a cell line for making vaccines; sequentially carrying out step-by-step magnifying microcarrier suspension culture on the CPB cells through stirring bottles and bioreactors, so that the CPB cells are used as hosts of virus infection; finally obtaining the siniperca chuatsi infectious spleen and kidney necrosis virus bulk and the siniperca chuatsi rhabdovirus bulk. The prepared ISKNV potency reaches up to 8.34LgTCID50 / mL, and the SCRV potency reaches up to 10.25LgTCID50 / mL. A microcarrier suspension culture process adopted by the invention is simple to operate, less in pollution probability, small in labor force compared with a roller bottle culture method, and lower in production cost.
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Description

technical field

[0001] The invention relates to a method for producing mandarin fish infectious spleen-kidney necrosis virus and mandarin fish fluke virus by culturing CPB cells with microcarriers, and belongs to the technical field of cell engineering. Background technique

[0002] Infectious Spleen and Kidney Necrosis Virus (ISKNV) is a disease that mainly breaks out in mandarin fish. It is caused by Infectious Spleen and Kidney Necrosis Virus (ISKNV) in the Iridoviridae genus Spleen and Kidney Necrosis Virus (ISKNV). 90%. The spleen and kidney of the sick mandarin fish had clinical symptoms such as hemorrhage and swelling. The disease brings huge economic losses to the mandarin fish farming industry every year.

[0003] Siniperca Chuatsi Rhabdovirus is caused by Siniperca Chuatsi Rhabdovirus (SCRV) in Rhabdoviridae, and the fatality rate exceeds 80%. The sick mandarin fish mainly showed clinical symptoms of skin hemorrhage. The disease brings huge economic losses to t...

Claims

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