A kind of infectious spleen and kidney necrosis virus orf069 gene deletion strain and its preparation method and application

A spleen and kidney necrosis virus, gene deletion technology, applied in microorganism-based methods, biochemical equipment and methods, viruses, etc., can solve problems such as unreported research, and achieve high immunization rate, cost saving, and good immunization effect. Effect
CN108384764BActive Publication Date: 2022-02-11SUN YAT SEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SUN YAT SEN UNIV
Publication Date
2022-02-11

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Abstract

The invention provides an infectious spleen-kidney necrosis virus ORF069 gene deletion strain and its preparation method and application, which is an attenuated virus strain prepared by deleting the ORF069 gene of the infectious spleen-kidney necrosis virus strain. The present invention also provides an infectious spleen and kidney necrosis virus ORF069 gene deletion strain with a selection marker and its preparation method and application, wherein the ORF069 gene is deleted for the infectious spleen and kidney necrosis virus, and homologous recombination is carried out at the gene deletion site An attenuated virus strain made by inserting a selection marker gene. The infectious spleen-kidney necrosis virus ORF069 gene deletion strain provided by the present invention is a recombinant genetically engineered vaccine in which the ORF069 gene has been knocked out. Immunity effect, without injection, great application value.
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Description

technical field

[0001] The invention relates to the field of aquaculture disease prevention and control, in particular to an infectious spleen and kidney necrosis virus ORF069 gene deletion strain and its preparation method and application. Background technique

[0002] Mandarin fish, also known as sweet-scented osmanthus fish, has become one of the main freshwater farmed fishes in my country because of its fast growth, good quality and delicious taste. The iridescent virus disease of mandarin fish caused by infectious spleen and kidney necrosis virus (ISKNV) is the main disease in mandarin fish farming, causing the mortality rate of mandarin fish to be close to 100%, which has become the main bottleneck restricting the development of mandarin mandarin fish farming.

[0003] Infectious spleen and kidney necrosis virus (ISKNV) is a representative species of the genus Dymovirus. The nucleic acid sequence of its entire gene has been determined. The total length is 111362bp, the...

Claims

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