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A kind of infectious spleen and kidney necrosis virus orf069 gene deletion strain and its preparation method and application

A spleen and kidney necrosis virus, gene deletion technology, applied in microorganism-based methods, biochemical equipment and methods, viruses, etc., can solve problems such as unreported research, and achieve high immunization rate, cost saving, and good immunization effect. Effect

Active Publication Date: 2022-02-11
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the study of ISKNV ORF069 gene deletion strain as gene deletion live attenuated vaccine

Method used

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  • A kind of infectious spleen and kidney necrosis virus orf069 gene deletion strain and its preparation method and application
  • A kind of infectious spleen and kidney necrosis virus orf069 gene deletion strain and its preparation method and application
  • A kind of infectious spleen and kidney necrosis virus orf069 gene deletion strain and its preparation method and application

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Embodiment Construction

[0052] The following description is a preferred embodiment of the present invention, it should be pointed out that for those skilled in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications are also considered Be the protection scope of the present invention.

[0053] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The experimental method that does not indicate specific condition in the embodiment, generally according to routine condition. Unless otherwise specified in the examples of the present invention, all reagents and consumables used are commercially available.

[0054] The embodiment of the present invention provides a preparation method of recombinant virus vaccine with ORF069 gene deletion of infectious spleen and kidney necrosis virus, comprising the following steps:

[00...

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Abstract

The invention provides an infectious spleen-kidney necrosis virus ORF069 gene deletion strain and its preparation method and application, which is an attenuated virus strain prepared by deleting the ORF069 gene of the infectious spleen-kidney necrosis virus strain. The present invention also provides an infectious spleen and kidney necrosis virus ORF069 gene deletion strain with a selection marker and its preparation method and application, wherein the ORF069 gene is deleted for the infectious spleen and kidney necrosis virus, and homologous recombination is carried out at the gene deletion site An attenuated virus strain made by inserting a selection marker gene. The infectious spleen-kidney necrosis virus ORF069 gene deletion strain provided by the present invention is a recombinant genetically engineered vaccine in which the ORF069 gene has been knocked out. Immunity effect, without injection, great application value.

Description

technical field [0001] The invention relates to the field of aquaculture disease prevention and control, in particular to an infectious spleen and kidney necrosis virus ORF069 gene deletion strain and its preparation method and application. Background technique [0002] Mandarin fish, also known as sweet-scented osmanthus fish, has become one of the main freshwater farmed fishes in my country because of its fast growth, good quality and delicious taste. The iridescent virus disease of mandarin fish caused by infectious spleen and kidney necrosis virus (ISKNV) is the main disease in mandarin fish farming, causing the mortality rate of mandarin fish to be close to 100%, which has become the main bottleneck restricting the development of mandarin mandarin fish farming. [0003] Infectious spleen and kidney necrosis virus (ISKNV) is a representative species of the genus Dymovirus. The nucleic acid sequence of its entire gene has been determined. The total length is 111362bp, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00C12N7/01C12N15/85A61K39/12A61P31/20C12R1/93
CPCA61K39/12A61P31/20C12N7/00C12N15/85C07K14/005A61K2039/5254A61K2039/552C12N2710/00051C12N2710/00034C12N2710/00022C12N2710/00021C12N2800/106
Inventor 何建国郭长军曾若云林易凡翁少萍何键
Owner SUN YAT SEN UNIV
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