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Infectious leenand kidney necrosis virus ORF022 gene deletion strain as well as preparation method and application thereof

A technology of spleen and kidney necrosis virus, ORF022, applied in biochemical equipment and methods, viruses, viral peptides, etc., can solve problems that have not been reported in research, and achieve high immunity rate, good immune effect, and cost-saving effects

Active Publication Date: 2018-06-29
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the use of the ISKNVORF022 gene deletion strain as a gene deletion live attenuated vaccine at present.

Method used

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  • Infectious leenand kidney necrosis virus ORF022 gene deletion strain as well as preparation method and application thereof
  • Infectious leenand kidney necrosis virus ORF022 gene deletion strain as well as preparation method and application thereof
  • Infectious leenand kidney necrosis virus ORF022 gene deletion strain as well as preparation method and application thereof

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Embodiment Construction

[0052] The following description is a preferred embodiment of the present invention, it should be pointed out that for those skilled in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications are also considered Be the protection scope of the present invention.

[0053] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The experimental method that does not indicate specific condition in the embodiment, generally according to routine condition. Unless otherwise specified in the examples of the present invention, all reagents and consumables used are commercially available.

[0054] The embodiment of the present invention provides a preparation method of recombinant virus vaccine with ORF022 gene deletion of infectious spleen and kidney necrosis virus, comprising the following steps:

[00...

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Abstract

The invention provides an infectious leenand kidney necrosis virus ORF022 gene deletion strain as well as a preparation method and the application thereof. The strain is an attenuated virus strain made by deleting an ORF022 gene of an infectious leenand kidney necrosis virus strain. The invention further provides an infectious leenand kidney necrosis virus ORF022 gene deletion strain with a screening marker, and a preparation method and the application thereof. The strain is an attenuated virus strain made by deleting the ORF022 gene of the infectious leenand kidney necrosis virus and inserting a screening marker gene into a gene deletion part through homologous recombination. The infectious leenand kidney necrosis virus ORF022 gene deletion strain provided by the invention is a recombinant gene engineering vaccine of which the ORF022 gene is knocked off, has relatively weak toxicity, has a remarkable immunity effect through only immersion immunization, needs no injection, and has verygreat application values, and the disease fatality rates of immunized fishes can be greatly reduced.

Description

technical field [0001] The invention relates to the field of prevention and control of aquaculture diseases, in particular to an infectious spleen and kidney necrosis virus ORF022 gene deletion strain and its preparation method and application. Background technique [0002] Infectious spleen-kidney necrosis virus (ISKNV) is a representative species of the genus Dymovirus. ISKNV causes infectious diseases after infecting the main host mandarin fish, and causes a large number of mandarin fish to die. The group nucleic acid sequence of the whole gene of ISKNV has been determined, its full length is 111362bp, G+C content is 54.78%, contains 124 potential open reading frames (ORF), and the coding length ranges from 40 to 1208 amino acids. Through sequence analysis, it is speculated that ORF006L is the main capsid protein; ORF19R, ORF46L, ORF27L and ORF63L are related to DNA replication, modification and processing; ORF24, ORF28L, ORF34R, ORF64L and ORF87R are related to transcri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01A61K39/12A61P31/20
CPCA61K39/12C07K14/005A61K2039/552A61K2039/5254C12N2710/00021C12N2710/00022C12N2710/00034
Inventor 何建国郭长军曾若云林易凡翁少萍何键
Owner SUN YAT SEN UNIV
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