30results about How to "Sensitive and specific" patented technology

The invention pertains to the clinical medicine field, disclosing a Mycobacterium tuberculosis DNA screening method which is based on a Loop-mediated isothermal amplification technology. A nucleic acid detecting method which is based on Loop-mediated isothermal amplification technical principle, detects the Mycobacterium tuberculosis in sputum. By adopting specific primers, and utilizing LAMP technology platform to amplify the specific area of target gene, the Mycobacterium tuberculosis in sputum is screened and detected in molecular level with the auxiliary of a series of quality control and internal control detecting system. The invention is characterized by simpleness and convenience, economy, quick speed, sensitivity and specific performance, and has wide application prospect.

The present invention is prawn white spot syndrome virus gene diagnosing kit and its usage. The kit and its usage is designed based on two pairs of primers for the conservation sequence of prawn white spot syndrome virus gene. By means of PCR technology, the present invention detects qualitatively the specific DNA nucleic acid segment of prawn white spot syndrome virus fast, simply, specifically and sensitively. The present invention may be used in the tracing detection of different stages of prawn cultivation and environment monitoring.

The invention relates to the field of biotechnology, in particular to a method for rapidly detecting Angiostrongylus cantonensis by utilizing the loop-mediated isothermal amplification (LAMP) technology for the rapid detection technology of verminosis. In the method, two pairs of LAMP primers (FIP, BIP, F3 and B3) for detecting Angiostrongylus cantonensis are provided, and an LAMP method for detecting Angiostrongylus cantonensis by utilizing the two pairs of primers is provided. The method comprises preparation of a reaction template, utilization of an LAMP reaction system and an LAMP reaction program and judgment of detection results. The invention has the advantages of simple and convenient operation, low cost and easy observation of results, and is very suitable for on-site rapid detection of Angiostrongylus cantonensis.

The present invention is mandarin fish infectious spleen and kidney necrosis virus gene diagnosing kit and its usage. The kit and its usage is designed based on two pairs of primers for the conservation sequence of mandarin fish infectious spleen and kidney necrosis virus gene. By means of PCR technology, the present invention detects qualitatively the specific DNA nucleic acid segment of mandarin fish infectious spleen and kidney necrosis virus fast, simply, specifically and sensitively. The present invention may be used in the tracing detection of different stages of mandarin fish cultivation and environment monitoring.

The invention relates to a fusion protein HABP-mKate and a preparation method and application thereof. The fusion protein HABP-mKate has the amino acid sequence disclosed as SEQ ID NO.2. The fusion protein HABP-mKate is prepared as follows: optimizing the gene sequence of HABP and mKate by gene optimization software; synthesizing the optimized gene sequence in vitro with an overlapping PCR method; adding a corresponding endonuclease site on two ends; cloning to a PET-15b expression vector; converting BL21-plus host bacteria; carrying out induction expression; separating expression bacteria after ultrasonication to obtain supernatant; filtering the supernatant obtained by separation by a filter membrane; carrying out affinity purification by a nickel post; eluting by 0.5M imidazole buffer solution; and obtaining the target protein HABP-mKate. The fusion protein HABP-mKate is used for detecting hyaluronic acid.

The invention relates to the technical field of virus detection and especially relates to an LAMP (loop-mediated isothermal amplification) primer for detecting porcine circovirus 3. The LAMP primer is shown by a sequence 1-6 in a sequence list. The LAMP primer is utilized to perform amplification; if an amplification result is obtained, a sample to be detected contains the porcine circovirus 3; if no amplification result is obtained, the sample to be detected has no the porcine circovirus 3. The method has the advantages of sensitivity, specificity, convenience, quickness and application to PCV3 detection and clinical diagnosis.

The invention provides a peste des petits ruminants virus antibody detection kit based on an immunoperoxidase monolayer assay (IPMA). The kit comprises a 96-well cell culture plate containing intracellular positive antigens and intracellular negative antigens, positive control serum, negative control serum, sample diluents, saline scrubbing solutions, HRP labeled mouse anti-goat McAb, two-component AEC colorimetric solutions, stop solutions, a serum diluting plate and an operating instruction. The intracellular positive antigens are BHK-gSLAM cells infected by a peste des petits ruminant virus rPPRV/GFP, the intracellular negative antigens are BHK-gSLAM cells not infected by the peste des petits ruminant virus, the positive control serum is immune positive serum obtained through blood sampling and separating after a goat is immunized by peste des petits ruminants virus vaccine strains PPRV N75/1, and the neutralizing titer is above 1:160; the negative control serum is negative serum obtained through direct blood sampling and separating from a non-immunized peste des petits ruminants negative goat.

The present invention comprises methods and apparatus for detecting prolonged myocardial repolarization as an indicator of cardiac conditions, including without limitation, transmural ischemia. In some embodiments, without limitation, the present invention comprises methods and apparatus to detect prolonged repolarization using electrocardiographic and electrophysiological tools and measurements.

The invention belongs to the field of gene engineering, and discloses detection and an application of a novel cervical cancer metastasis marker. The cervical cancer metastasis marker is a lymphatic metastasis mode LVEM wrapped by tumor-associated macrophages, and is applied to diagnosis for distinguishing metastatic and non-metastatic early cervical cancers. Aiming at defects that an existing method for evaluating preoperative early cervical cancer lymph node metastasis is not high in sensitivity, has limitation on detection of micro-metastatic lymph nodes, is not high in timeliness and economic practicability, has false negative or false positive, is not high in sensitivity and specificity of the existing tumor marker and the like, based on a fact that an expression level of the LVEM canserve as an index for evaluating the lymph node metastasis state of a cervical cancer patient, the LVEM is developed as a marker, the expression level of the LVEM in cervical cancer tissue is detectedthrough a multicolor immunofluorescence technology, and the lymph node metastasis state of the cervical cancer patient can be more sensitively and specifically evaluated before an operation.

The invention discloses a female premature ovarian insufficiency susceptibility gene detection model and a detection kit. The female premature ovarian insufficiency susceptibility gene detection modelprovided by the invention is composed of 9 SNP loci of four genes of SCUBE1, GPSM1, CNN2 and PSPH. According to the invention, a gene detection model especially for Chinese females is established forthe first time, which has race specificity. Moreover, a detection reagent and a kit researched and developed according to the gene detection model disclosed by the invention can be used for accurately, sensitively and specifically detecting the premature ovarian insufficiency of Chinese women; and a new way and scheme are provided for detecting the premature ovarian insufficiency of Chinese females.

The application discloses a female premature ovarian failure susceptibility gene detection model and detection kit. The female premature ovarian failure susceptibility gene detection model disclosed by the application consists of SNP loci of fifteen genes including ELN, SOD2, FSHR, DENND1A, IL17RD, FGF8, KAL1, THADA, LHCGR, FGFR1, GLO1, BMP15, KISS1R, ESR1 and CAT. According to the application, the gene detection model especially for Chinese females is initiatively established, and has race specificity. Moreover, a detection reagent and a kit which are researched and developed according to thegene detection model disclosed by the application can be used for carrying out accurate, sensitive and specific detection on the premature ovarian failure of Chinese females; and a new approach and scheme are provided for premature ovarian failure detection of Chinese females.

The invention relates to a parasite quick detection technology in the technical field of biology, and discloses a specific primer in LAMP (looped-mediated isothermal amplification) detection of trypanosoma evansi variant strain with deletion of a ToTat1.2 gene. The specific primer comprises two pairs of LAMP primers FIP, BIP, F3 and B3 for detecting trypanosoma evansi. An LAMP method using the two pairs of primers to detect the trypanosoma evansi comprises the following steps of preparation of a reaction template, using of an LAMP reaction system and an LAMP reaction procedure, and judgment of detection results. The specific primer has the advantages that the operation is simple and convenient, the cost is low, the result is easily observed, and the specific primer is special for the detection of the trypanosoma evansi variant strain with deletion of the ToTat1.2 gene.

The invention relates to the technical field of duck tembusu virus nucleic acid detection, particularly discloses a duck tembusu virus nucleic acid CRISPR-Cas13a detection system, an RPA primer pair and crRNA, and establishes a visual, sensitive and specific duck tembusu virus rapid clinical detection system based on a CRISPR-Cas13a diagnosis platform based on the designed RPA primer pair and the specific crRNA. The system does not need a complex temperature control instrument in the whole reaction process, isothermal detection only needs to be carried out at 37 DEG C, the system can be used for fluorescence reading or visual reading, the lowest detection limit of the detection system is 1.0 * 100 copies/reaction, cross reaction with other poultry viruses is avoided, the Cas13a detection system can also directly play a role in virus detection of clinical samples, and the Cas13a detection system can be used for detecting the viruses of the poultry. The kit has the advantages of simplicity, convenience and rapidness in detection operation, suitability for field detection, high specificity, high detection sensitivity, high accuracy and reliability.