Kit for diagnosing gene of pathogenic bacterial and river vibrion of aquatic animal and human and testing method thereof

Abstract

The invention provides a gene diagnosis reagent box and its detecting method for aquatic animal (like cyprinoid, Penaeus monodon, Epinephelus awoara, ormer, etc) and human pathogens-Vibrio fluvialis, designed by using a pair of primers designed by the sequence in conservative region of Vibrio fluvialis gene to act as main body. It adopts polyase chain reaction (PCR) technique to qualitatively detect the specific DNA fragments of the Vibrio fluvialis, simply and conveniently, and rapidly, good-specificity, and high-sensitivity; can be used in bacteria tracking detection of aquatic animal pathogens in the course of breeding in each period, and also the clinic detection of human intestinal acute infections, as well as environmental monitoring, avoiding the pathogens infecting and popularizing and it has very great practical value.

Description

technical field

[0001] The invention relates to a diagnostic kit and a detection method for aquatic economic animals and human pathogenic bacteria, and mainly relates to a kit and a detection method for gene diagnosis of Vibrio fluvialis. Background technique

[0002] Vibrio fluvialis is a halophilic bacterium widely distributed in rivers, bays and coastline areas and in seafood all over the world. This bacterium is an opportunistic pathogenic bacterium, which can break out and become popular under certain conditions. In recent years, with the deterioration of the breeding environment, there have been more and more reports of aquatic product morbidity or mass death and human food poisoning caused by Vibrio riverina in various regions of my country. At present, it is known that Vibrio fluvialus can cause many types of aquatic animals such as silver carp, carp, crucian carp, medlar, red carp, bream, etc. The bacteria can infect humans through contaminated aquatic products, c...

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