The invention discloses a
bacillus subtilis strain WN0101 and an application of the
bacillus subtilis strain WN0101. The
bacillus subtilis WN0101 is classified and named as bacillus subtilis, the bacillus subtilis WN0101 is preserved in the China Center for Type Culture Collection on September 29, 2025, and the preservation number of the bacillus subtilis WN0101 is CCTCC (China Center for Type Culture Collection) NO: M 20252145. The
fermentation supernatant of the bacillus subtilis WN0101 can be used for specifically killing
vibrio fluvialis and
streptococcus iniae. In addition, the bacillus subtilis WN0101 has no hemolytic activity, and the
fermentation supernatant of the bacillus subtilis WN0101 has good temperature stability and
protease stability. Therefore, the bacillus subtilis WN0101 can replace
antibiotics and is applied to prevention and treatment of
vibrio fluvialis and
streptococcus iniae infection.