10 results about "Vibrio fluvialis" patented technology

The invention belongs to the technical field of molecular biology, and particularly relates to a nucleic acid sequence combination for simultaneously detecting various pathogenic vibrios, an RT-PCR (reverse transcription-polymerase chain reaction) kit and a use method of the RT-PCR kit, and the nucleic acid sequence combination is shown as SEQ ID No.1-SEQ ID No.19. The RT-PCR kit comprises RT-PCR primer probes, the primer probes comprise forward and reverse amplification primers and detection probes respectively aiming at vibrio cholerae, vibrio parahaemolyticus, vibrio fluvialis, vibrio mimicus, vibrio vulnificus and vibrio alginolyticus, and corresponding sequences in SEQ ID No.1-SEQ ID No.19 are adopted. The kit disclosed by the invention can be used for rapidly detecting and identifying the six vibrios at the same time; the kit has high accuracy, specificity and sensitivity, and is suitable for clinical detection of infected samples.

The invention provides a method for detecting three pathogenic vibrios of penaeus vannamei. According to the method, specific primers are designed by using toxR genes of vibrio fluvialis, flaA genes of vibrio anguillarum and pyrH genes of vibrio alginolyticus, and sensitivity test is carried out. The lowest detectable concentrations of the three vibrios are 5.21 * 10 < 2 > cfu / mL, 2.70 * 10 < 4 > cfu / mL and 2.48 * 10 < 2 > cfu / mL, and the sensitivity is high. The three vibrios are accurately and simultaneously identified through a multiple PCR method, and the identification efficiency is improved. Meanwhile, the lowest detection concentrations of the DNA of the three vibrios identified and extracted by using a real-time fluorescent quantitative PCR (qRT-PCR) technology are 1.0 * 10 <-6 > nmol / L, 1.0 * 10 <-7 > nmol / L and 1.0 * 10 <-8 > nmol / L respectively. Compared with the two methods, the common PCR technology has lower requirements on instruments and operation, so that the common PCR technology is more suitable for the diagnosis of the vibrio infection of the penaeus vannamei. The qRT-PCR technology is more suitable for detection cases with higher sensitivity requirements. By using the method, an efficient and reliable means is provided for diagnosing the bacterial diseases of the penaeus vannamei boone.

The invention relates to a primer and probe combination, a kit and a method for detecting gastrointestinal tract pathogenic bacteria. The gastrointestinal pathogenic bacteria comprise vibrio cholerae, vibrio parahaemolyticus, salmonella, shigella, diarrheagenic escherichia coli, campylobacter, yersinia enterocolons, clostridium difficile, cronobacter sakazakii, aeromonas hydrophila, plesiomonas shigelloides and vibrio fluvialis. The primer and the probe group can be independently used for PCR amplification detection and can be combined in the same reaction system and reaction procedure for multiple PCR amplification detection, and the primers and the probes aiming at different pathogenic bacteria cannot interfere with each other during multiple PCR amplification detection. The primer and probe combination covers various types of gastrointestinal pathogenic bacteria, and is high in sensitivity, low in detection limit, good in specificity and wide in application prospect.

The application discloses specific molecular targets of Vibrio alginolyticus and Vibrio fluvialis and a detection method thereof, wherein the nucleotide sequence of the specific molecular target of the Vibrio alginolyticus is as shown in SEQ ID NO. 1, and the nucleotide sequence of the specific molecular target of the Vibrio fluvialis is as shown in SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 or SEQ ID NO. 5. The application not only discloses one specific molecular target for identifying the Vibrio alginolyticus and four specific molecular targets for identifying the Vibrio fluvialis, but also provides related specific primer sequences and corresponding PCR detection methods. The application has the advantages of short detection time, low cost, strong specificity and the like without physiological and biochemical identification.

The invention discloses a bacillus subtilis strain WN0101 and an application of the bacillus subtilis strain WN0101. The bacillus subtilis WN0101 is classified and named as bacillus subtilis, the bacillus subtilis WN0101 is preserved in the China Center for Type Culture Collection on September 29, 2025, and the preservation number of the bacillus subtilis WN0101 is CCTCC (China Center for Type Culture Collection) NO: M 20252145. The fermentation supernatant of the bacillus subtilis WN0101 can be used for specifically killing vibrio fluvialis and streptococcus iniae. In addition, the bacillus subtilis WN0101 has no hemolytic activity, and the fermentation supernatant of the bacillus subtilis WN0101 has good temperature stability and protease stability. Therefore, the bacillus subtilis WN0101 can replace antibiotics and is applied to prevention and treatment of vibrio fluvialis and streptococcus iniae infection.

The application discloses Vibrio fluvialis JH-3, which is preserved in the Guangdong Microbial Culture Collection Center and has a preservation number of GDMCC NO: 66094. The application also discloses application of the Vibrio fluvialis JH-3 in denitrification and nitrogen removal. The application further discloses a denitrification and nitrogen removal method for salt-containing wastewater, which comprises the following steps: mixing the Vibrio fluvialis JH-3 with salt-containing wastewater to obtain a mixed solution, and culturing to carry out denitrification and nitrogen removal. The Vibrio fluvialis JH-3 screened by the application has strong salt-tolerant denitrification capacity and can be used for biological nitrogen removal of high-salt wastewater.

The invention discloses a vibrio fluvialis JH-3 which is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the vibrio fluvialis JH-3 is GDMCC (China General Microbiological Culture Collection Center) NO: 66094. The invention further discloses an application of the vibrio fluvialis JH-3 in nitrogen removal by denitrification, and the vibrio fluvialis JH-3 can be used for preparing a nitrogen removal agent for nitrogen removal by denitrification. The invention also discloses a denitrification nitrogen removal method of the salt-containing wastewater, which comprises the following steps: uniformly mixing the Vibrio fluvialis JH-3 with the salt-containing wastewater to obtain a mixed solution, and culturing to perform denitrification nitrogen removal. According to the invention, the Vibrio fluvialis JH-3 is screened out, and the Vibrio fluvialis JH-3 has relatively strong salt-resistant denitrification capability and can be used for biological nitrogen removal of high-salt wastewater.

The invention discloses a primer probe group, a kit and a method for detecting black sea vibrio. The primer probe group capable of specifically detecting the vibrio black sea vibrio is designed based on the recA gene of the vibrio black sea vibrio as a target, and the detection sensitivity reaches 0.01 fg / mu L. The invention not only provides a new molecular target for detecting the vibrio black sea vibrio, but also can directly and specifically distinguish the vibrio black sea vibrio from vibrio parahaemolyticus, vibrio harveyi, vibrio fluvialis, vibrio alginolyticus and vibrio cholerae through PCR reaction, and provides a simpler and more convenient detection means for disease monitoring of the vibrio black sea vibrio in the aquaculture process.

The application discloses a method for treating printing and dyeing wastewater by using Chlorella sorokiniana and Vibrio fluvialis. 7 8 The method is to separate original Chlorella sorokiniana strains and Vibrio fluvialis strains from printing and dyeing wastewater, and then add Vibrio fluvialis bacterial liquid with a concentration of 2.3*10 7 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10 8 ~2.3*10

The invention provides a molecular target spot for specifically detecting vibrio fluvialis, a detection method and application, a primer group capable of specifically detecting vibrio fluvialis is designed by taking a gene segment with a nucleotide sequence as shown in SEQ ID NO: 1 or a gene segment with a nucleotide sequence as shown in SEQ ID NO: 2 as a molecular target. A new molecular target is provided for detection of vibrio fluvialis, meanwhile, the established detection method can rapidly identify vibrio fluvialis in a to-be-detected sample, and the method has wide application prospects in detection, prevention and treatment of vibrio fluvialis.