43results about How to "Avoid spreading the epidemic" patented technology

The invention discloses primer pairs, a detection method and a fast diagnostic kit for detecting red-spotted grouper nervous necrosis viruses. The primer pairs comprise the following four primers: an inner primer FIP, an inner primer BIP, an external primer F3 and an external primer B3. Both the primer pairs and the kit of the invention can detect the red-spotted grouper nervous necrosis viruses with high efficiency and high specificity, as well as are based on loop-mediated isothermal amplification technology, apply six segments, four primers and one constant temperature, complete an amplification reaction within 1 hour, and are low in detection cost, short in detection time, high in yield and specificity, obvious in negative and positive result color developing difference, high in verification rate, obvious and reliable in verification. The kit is provided with a set of optimized reverse transcription-loop-mediated isothermal amplification reaction system can be used for detecting red-spotted grouper nervous necrosis viruses at different stages in a fish culture process, avoids viral spread and prevalence, improves scientific management efficiency and has high practical value.

The present invention is prawn white spot syndrome virus gene diagnosing kit and its usage. The kit and its usage is designed based on two pairs of primers for the conservation sequence of prawn white spot syndrome virus gene. By means of PCR technology, the present invention detects qualitatively the specific DNA nucleic acid segment of prawn white spot syndrome virus fast, simply, specifically and sensitively. The present invention may be used in the tracing detection of different stages of prawn cultivation and environment monitoring.

The invention discloses a biological prevention and control method for controlling prawn diseases through Tilapia. The method of the invention realizes the prevention for infectious diseases of the prawn and the increase of prawn survival rate by introducing Tilapia to prawn cultivation zones. The method of the invention can prevent prawn diseases from propagating and becoming epidemic in healthy prawn community, reduce the workload of manually removing diseased and dead prawns, enhance the removal efficiency and the cultivation success rate and bring tremendous economical benefit to the culturist.

The present invention is mandarin fish infectious spleen and kidney necrosis virus gene diagnosing kit and its usage. The kit and its usage is designed based on two pairs of primers for the conservation sequence of mandarin fish infectious spleen and kidney necrosis virus gene. By means of PCR technology, the present invention detects qualitatively the specific DNA nucleic acid segment of mandarin fish infectious spleen and kidney necrosis virus fast, simply, specifically and sensitively. The present invention may be used in the tracing detection of different stages of mandarin fish cultivation and environment monitoring.

A kit used for diagnosing reovirus of juyuanqin crab consists of a pair of primer designed according to RNA sequence of a reovirus separated out from ill juyuanqin crab. Its detection method utilizes reverse transcription - polymerase chain reaction technique to carry out qualitative detection on specific RNA nucleic acid section of reovirus on juyuanqin crab.

The invention discloses a detection primer group, a detection kit, and a detection method of nocardia seriolae, vibrio harveyi, and streptococcus iniae. The detection primer group comprises a primer pair Ns-mce, a primer pair Vh-tox, and a primer pair Si-its; the nucleotide sequences of the primer pairs are represented by SEQ ID NO.1-6. The detection kit comprises above detection primer group. According to the detection method, the detection kit is used for detection; samples are subjected to pretreatment, genome DNA is extracted as sample group DNA, and PCR is adopted to detect the samples. A triplex PCR detection method is established for tracking measurement of nocardia seriolae, vibrio harveyi, and streptococcus iniae at different periods, avoiding transmission of pathogenic bacteria, and improving rapid inspection and quarantine of pathogenic bacteria in aquatic products; and the invention belongs to the field of aquatic cultured animal pathogenic bacteria detection.

The invention relates to a kit for diagnosing the reovirus genes of grass carps, comprising an RNA (Ribonucleic Acid) extraction reagent for viruses in visceral tissues, an RAN extraction reagent for cell infecting viruses, an RT (Reverse Transcriptase) reaction reagent and a PCR (Polymerase Chain Reaction) reaction reagent, wherein the RNA extraction reagent for the viruses in the visceral tissues comprises the components of DEPC (Diethyl Pyrocarbonate) water, protease K (1 mg/mL, pH is 8.0), SDS (Sodium Dodecyl Sulfate) (1%), phenol/chloroform/isoamylol (25/24/1), chloroform/isoamylol (24/1), isopropanol and 70% ethanol; the RAN extraction reagent for the cell infecting viruses comprises the components of Trizol, chloroform, isopropanol, 70% ethanol and the DEPC water; the RT reaction reagent comprises the components of 5*AMV Buffer, dNTP, the DEPC water, a reverse primer R1, an RNA enzyme inhibitor RNAsin (R1) and a reverse transcriptase AMV; and the PCR reaction reagent comprises the components of 10*PCR buffer (containing Mg<2+>) and DNTP Mixture (which are respectively 2.5 mmol/L), a specific oligonucleotide primer F1, the primer R1, the DEPC water and Taq E (5U/muL), the forward primer F1:5'-ATCCCGTATATCTATGGCTT-3', and the reverse primer R1:5'TTGGAGACGAACATAGACGC-3. The kit is used for diagnosing the reovirus genes of the grass carps.

The invention discloses a quick diagnostic kit for abalone shriveling syndrome associated virus (AbSV). The quick diagnostic kit comprises the following components: (1) DNA extract; (2) digestive juice; (3) G1 liquid used for polymerase chain reaction (PCR) amplification reaction and comprising Buffer containing Mg<2+>, dNTP, forward primer F1, reverse primer R1 and Taq enzyme, wherein F1 is 5-ATGACAGATTTCACCGTAAGCAATGAAAATC-3, and R1 is 5-CTAGCTGGCTTTGGTATAAGTAGTACCAAAAG-3; and (4) positive control H liquid, which is 100ng/muL of AbSV infected abalone genome DNA. The invention also disclosesa method for detecting the AbSV by using the quick diagnostic kit. The quick diagnostic kit and the detection method provided by the invention can be used for virus tracking detection of each period in the abalone culture process, can also be used for environment monitoring, avoid viral transmission, improve the scientific management efficiency, and have a very high practical value.

The invention discloses a biological prevention and control method for controlling prawn diseases with clarias lazera. According to the method provided by the invention, clarias lazeras are added into a prawn culture zone, such that prawn infectious disease prevention is realized, and prawn survival rate is improved. With the method, prawn diseases can be prevented from spreading in healthy prawn populations; work load of artificially removing diseased and dead prawns is reduced; removing efficiency and culture success rate are improved; and higher economic benefit is brought to farmers.

The invention provides a biological control method of controlling prawn diseases through grass carps. The method is characterized by putting grass carps in a prawn culture area to prevent the infectious diseases of prawns and increase the survival rate of prawns. The method can prevent the spreading and prevalence of prawn diseases in healthy prawn groups, reduce workload for removing diseased prawns and dead prawns manually, increase the removal efficiency and culture success rate, and bring greater economic benefit for culturists.

The invention discloses a primer set for testing mud crab bicistronic mRNA virus, a test method and a rapid diagnosis kit. The primer set includes the following four primers: outer primer F3, outer primer B3, inner primer FIP and inner primer BIP. The primer set for testing mud crab bicistronic mRNA virus, the test method and the rapid diagnosis kit can test mud crab bicistronic mRNA virus at high efficiency and high specificity, the specificity of the primer set is high, and the test method has the advantages of low test cost, short time consumption, high productivity, high specificity, remarkable color difference between negative and positive results and high verification rate, and is clearer and more reliable; and the kit in the invention establishes a set of optimized inverse transcription-loop-mediated isothermal amplification reaction system, can be used for tracking and testing mud crab dbicistronic mRNA virus at each stage in the process of culturing blue crabs, and can prevent the spread of virus, so the efficiency of scientific management is increased, and the high practical value is realized.