40results about How to "Significant difference in color rendering" patented technology

The invention provides a mulberry silkworm pebrine test kit. The mulberry silkworm pebrine (Nb) test kit comprises two pairs of primers which are designed by taking mulberry silkworm pebrine spores as target genes on the basis of a loop-mediated isothermal amplification (LAMP) technology, namely external primers F3/B3 and internal primers FIP/BIP. The mulberry silkworm pebrine test kit has a more comprehensive detection effect and low omission rate and can replace the conventional microscope detection technologies.

The invention discloses a rapid diagnosis kit and a detection method of genes of comma bacillus. The kit consists of two pairs of primers, DNA polymerase, stabilizing solution, reaction solution, sample pre-treatment solution, color development solution and positive comparison solution, wherein, the seven solutions are respectively placed in containers. The rapid diagnosis kit of genes of the invention applies six sections and four primers and can judge whether target materials exist only according to the amplification, thus being high in specificity. The rapid diagnosis kit of genes of the invention is high in speed, high in efficiency and sensitivity, can amplify the reaction with only constant temperature, without special reagents or equipment, and is low in detection cost. The rapid diagnosis kit of genes of the invention is simple and convenient in identification. Pyrophosphate ions separated out from the dNTP are combined with Mg<2+> in the reaction solution to produce a by-product which is the sediment of magnesium pyrophosphate which can be identified by naked eyes. Furthermore, after the color development solution is added, the color development differences of negative and positive results are notable and the identification is more obvious and reliable.

The invention discloses a primer group and a kit for detecting Roundup Ready transgenic soybeans. The kit consists of the primer group, Bst deoxyribonucleic acid (DNA) polymerase, a stabilizing solution, a reaction solution, a developing solution and a positive control solution. In the kit, six segments and four primers are utilized; and the kit has high specificity according to the condition that whether the existence of target materials can be judged or not through amplification. The quick diagnostic kit provided by the invention has high speed, high efficiency and high sensitivity, can be subjected to an amplification reaction only by a constant temperature without the usage of special reagents and equipment, has low detection cost and is easy and convenient to identify; pyrophosphate ions precipitated from deoxyribonucleoside triphosphate (dNTP) is combined with Mg<2+> in the reaction solution; the produced byproduct, namely magnesium pyrophosphate is precipitated and can be observed and identified through naked eyes; and after the developing solution is added, the kit has obvious development differences of negative and positive results and is more obvious and reliable. A soybean endogenous gene lectin is used as an internal label, so that errors and false negatives caused by the extraction of nucleic acid are prevented; and real-time monitoring is performed, so that detection time is greatly reduced, and human power and material resources are saved.

The invention discloses a visualized rapid detection kit for carp dropsy virus and a detection method thereof. The visualized rapid detection kit for the carp dropsy virus includes a virus DNA extraction reagent and a reaction reagent, the reaction reagent contains a nucleic acid primer set used for detecting amplification of the carp dropsy virus, and the primer set contains a primer CEV-F3, a primer CEV-B3, a primer CEV-BFIP, a primer CEV-BIP, a primer CEV-LpF and a primer CEV-LpB. The rapid detection kit establishes a set of optimized isothermal amplification reaction system, the qualitative detection of the carp dropsy virus is thus simpler, faster, high in specificity and high in sensitivity, and the kit is the first kit used for detecting the carp dropsy virus, the problem that there is no detection method for the carp dropsy virus is solved, and has high scientific and economic value.

The invention provides a mononuclear hyperplasia Listeria monocytogenes gene quick diagnosis reagent box based on a loop-mediated isothermal amplification technology and a method for detecting the same. The reagent box consists of two pairs of primers, DNA polymerase, reaction liquid, sample pretreatment liquid, developing liquid and a positive contrast solution, and the six kinds of liquid are respectively contained in a container. The gene quick diagnosis reagent box adopts six segments and four primers, and can judge whether a target substance exists or not according to the fact whether amplification occurs or not, thereby having high specificity. Moreover, the reagent box has quickness, high efficiency and high sensitivity, and can carry out amplification reaction just at a constant temperature without adopting special reagent and equipment. In addition, the reagent box can realize simple identification; therefore, pyrophosphate radical ion separated out from dNTP is combined with Mg<2+> in a reaction solution so as to generate byproduct magnesium pyrophosphate precipitate which can be identified through unaided viewing; moreover, after the developing liquid is added, the remarkable positive and negative result color developing difference is more obvious and reliable.

The invention relates to a kit for detecting swill-cooked dirty oil. The kit comprises a detection primer solution prepared from an outer primer I, an outer primer II, an inner primer I and an inner primer II, a BstDNA polymerase with chain displacement activity, a 10*reaction buffer, a dNTPs solution, a positive DNA control sample and a negative DNA control sample. Meanwhile, the invention also discloses a detection method of the kit. The kit disclosed by the invention has the characteristics of high specificity, high speed, high efficiency and low detection cost, is simple and convenient to operate and is suitable for rapid field detection.