114 results about "River prawn" patented technology

The invention discloses a macrobrachium rosenbergii iridovirus disease resistance-related SNP (Single Nucleotide Polymorphism) site, a site combination and application thereof, and belongs to the field of aquatic organism breeding. According to the invention, SNP loci related to resistance of the macrobrachium rosenbergii iridovirus disease are successfully screened through whole genome association analysis, and the SNP loci are respectively located at a 9574545th base of a chromosome 10 of the macrobrachium rosenbergii and a 26491028th base of a chromosome 14 of the macrobrachium rosenbergii. Aiming at the two SNP sites, an SNP site combination related to the resistance of the macrobrachium rosenbergii iridovirus disease is further developed, and macrobrachium rosenbergii iridovirus disease resistant individuals can be screened by detecting the SNP site combination. Therefore, the SNP molecular marker provided by the invention can be used for macrobrachium rosenbergii molecular marker assisted breeding, accelerates disease-resistant macrobrachium rosenbergii variety breeding, and provides a reference basis for disease-resistant breeding work of macrobrachium rosenbergii.

The invention discloses application of astaxanthin as a feed additive in improving the disease resistance of macrobrachium rosenbergii, and belongs to the field of feed additives. According to the method, astaxanthin is supplemented in the feed, the survival rate of the juvenile macrobrachium rosenbergii after being infected with DIV1 is increased, meanwhile, the abundance of pathogenic bacteria in intestinal tracts after the macrobrachium rosenbergii is infected with DIV1 is reduced, then the purpose of preventing disease outbreak of the macrobrachium rosenbergii caused by DIV1 can be achieved, and the method is a sustainable and effective method for preventing and treating the macrobrachium rosenbergii DIV1 disease.

The invention discloses lactococcus gasseri C6a2 and application thereof. The preservation number of the lactococcus gasseri C6a2 is CGMCC (China General Microbiological Culture Collection Center) NO: 31024; the 16S rRNA (ribosomal Ribonucleic Acid) gene sequence of the lactococcus gasseri C6a2 is as shown in SEQ ID NO: 1. The bacterial strain is a macrobrachium rosenbergii intestinal absolute dominant bacterium, has a typical probiotic function, is high in growth speed and activity, can quickly enter a logarithmic phase, has relatively high acid production and acid resistance, can secrete lipase, has relatively high bacteriostatic activity on staphylococcus aureus, aeromonas hydrophila and aeromonas veronii, and can be used for preparing the macrobrachium rosenbergii. The bacterium can be used for preparing a special probiotic preparation for macrobrachium rosenbergii, so that the sustainable development of the macrobrachium rosenbergii industry in China is promoted.

The invention discloses a feed functional compound for improving salt-alkali stress resistance of macrobrachium rosenbergii and application of the feed functional compound, and belongs to the technical field of aquaculture. The feed functional compound for improving saline-alkaline stress resistance of macrobrachium rosenbergii provided by the invention comprises vitamin D3, betaine and sodium oleate. According to the invention, the absorption promotion effect of vitamin D3 on calcium and phosphorus ions is utilized, so that the ion metabolism disorder of macrobrachium rosenbergii under saline-alkali stress is improved; by utilizing the osmotic pressure regulation capability of betaine, osmotic pressure unbalance under saline-alkali stress is improved, and the energy consumption of osmotic regulation is reduced; the sodium oleate is used for improving energy supply, the membrane structure is adjusted to improve osmotic pressure adjusting capacity, and the three components act together to achieve the purpose of improving salt-alkali stress tolerance of macrobrachium rosenbergii.

A macrobrachium rosenbergii breeding method belongs to the technical field of shrimp breeding, and comprises the following steps: selection of parent shrimps: selecting a macrobrachium rosenbergii pseudo-female population introduced from Thailand as female shrimps, and stocking in a breeding pond A; screening domestic male groups which are robust in physique and are not carried by viruses, and stocking the male groups in the breeding pond B; thailand pseudo female shrimps and domestic male shrimps are subjected to cross breeding, and the domestic male shrimps (South Taihu No.3) are subjected to cross breeding with the Thailand pseudo female shrimps by virtue of relatively high low temperature resistance, so that the low temperature resistance of hybrid offspring is improved, and the current breeding situation of Thailand Macrobrachium macrobrachium offspring seeds in China is improved.

The invention provides a macrobrachium rosenbergii quality grading system based on machine vision. The macrobrachium rosenbergii quality grading system based on machine vision comprises a prawn body placing platform used for placing macrobrachium rosenbergii to be tested; the three-dimensional acquisition module comprises a structured light projection device, a binocular stereo camera and a flight time depth sensor; and the three-dimensional reconstruction module is used for constructing the data of the three-dimensional acquisition module into a complete three-dimensional point cloud model through a data fusion algorithm and a point cloud registration algorithm. According to the macrobrachium rosenbergii quality grading system based on machine vision, the overall scheme has robustness and expandability, the system is suitable for various large-scale farms and grading scenes, and the intelligent level of macrobrachium rosenbergii quality control is greatly improved.

The invention discloses a macrobrachium rosenbergii cherax ratio prediction and breeding method based on phenotypic omics analysis, which comprises the following steps: selecting a plurality of macrobrachium rosenbergii for multi-generation breeding, recording pedigree data of each individual, and constructing a macrobrachium rosenbergii breeding database; the method comprises the following steps: extracting phenotypic characteristics of macrobrachium at different culture stages, analyzing a rule between a growth index and a chelate ratio, carrying out heritability evaluation, and constructing a macrobrachium phenotypic omics network; the growth path of macrobrachium rosenbergii is analyzed, abnormal events in the breeding period are recognized, and a macrobrachium rosenbergii growth situation network is generated; constructing a macrobrachium phenotype knowledge map, and constructing a macrobrachium cherax ratio prediction model; and performing data acquisition on a macrobrachium rosenbergii population to be bred to obtain early phenotypic data, performing breeding decision, and performing breeding path recommendation on bred individuals by using the macrobrachium rosenbergii phenotypic knowledge graph. Meanwhile, whether the breeding path is deviated or not in the breeding process is analyzed, and a correction scheme is formulated for pushing, so that the breeding period of the macrobrachium is shortened, and the breeding accuracy and efficiency are improved.

The invention discloses an SNP molecular marker related to growth of macrobrachium rosenbergii under the condition of ingestion of low-protein feed and application of the SNP molecular marker, and relates to the field of genetic breeding of macrobrachium rosenbergii. A nucleotide sequence containing the SNP molecular marker is shown as SEQ ID NO.1, the nucleotide sequence is located at the 501st site from the 5'end on the SEQ ID NO.1 and corresponds to the 76447836th site on the 10th chromosome of the macrobrachium rosenbergii, and the polymorphic site of the SNP molecular marker is C or T. The SNP molecular marker is closely related to the body weight and body length of the macrobrachium rosenbergii under the condition of ingestion of the low-protein feed, so that the locus can provide reference for screening and cultivating new varieties of the macrobrachium rosenbergii resistant to the low-protein feed.

The invention discloses a compound feed for improving the survival rate of juvenile macrobrachium rosenbergii, and belongs to the technical field of aquatic animal breeding. The fish meal, the soybean meal, the fermented soybean meal, the algae, the starch, the fat, the compound amino acid, the compound vitamin, the mineral substances and the immune polysaccharide are used as raw materials, the fish meal, the soybean meal and the fermented soybean meal are used for providing protein needed by growth of the juvenile shrimps, and the soybean meal and the fermented soybean meal can replace part of the fish meal, so that healthy growth of the juvenile shrimps is guaranteed, and meanwhile, the feed cost is reduced; the addition of algae, immune polysaccharide and compound amino acid can enhance the immunity and anti-stress ability of the juvenile shrimps, and also can improve the utilization rate of protein and other nutritional ingredients, thereby improving the feed efficiency; meanwhile, starch, fat, multivitamins and mineral substances are added to provide nutritional ingredients for healthy growth of the juvenile shrimps, and rapid growth and healthy development of the juvenile shrimps are promoted; and the survival rate of juvenile macrobrachium rosenbergii is increased through the combined action of the components.

The invention relates to the technical field of phenotype measurement in aquatic genetic breeding, in particular to a macrobrachium rosenbergii small-size shrimp high-throughput phenotype measurement method and measurement system. According to the method, a camera obscura environment is constructed through a white shooting bottom plate, a dark extinction inner wall and an LED area array light source in a measuring box body, and illumination is adaptively adjusted by combining rough segmentation of a preview and analysis of a local gray histogram, so that stable bimodal gray distribution is formed between a shrimp body and a background. On the basis, a segmentation-key point combined deep learning model is adopted, a shrimp body mask and a standard key point are output at the same time, topological consistency loss constraints of a framework side length proportion and an included angle are introduced in training, and key point mismarking and drifting caused by the transparent body color are remarkably reduced. According to the method, rapid, non-contact, high-precision and high-throughput measurement of macrobrachium rosenbergii small-specification individual phenotype parameters and weight is realized, and a high-quality structured phenotype database is provided for genetic evaluation and multi-character combined selection.

The invention discloses a macrobrachium rosenbergii probiotic biofloc technology (BFT) culture system construction method based on an SBOS-FOS composite carbon source, and belongs to the technical field of aquaculture. The total ratio of SBOS (soybean oligosaccharide) and FOS (fructo-oligosaccharide) in the composite carbon source is 2%-10%, and the balance is glucose; and adding the composite carbon source into a macrobrachium rosenbergii culture system according to the C / N ratio of (10: 1)-(20: 1) according to the feed protein content in the culture water body. Through a specific ratio of SBOS to FOS, the content of functional probiotics in a culture water body is specifically increased, stable control of ammonia nitrogen and nitrite nitrogen and directional optimization of a carbon source-flora-host three-level regulation and control network in a BFT system are realized, the weight gain rate (WG), the specific growth rate (SGR) and digestive enzyme activity (trypsin and amylase) of macrobrachium rosenbergii are increased, and the survival rate of the macrobrachium rosenbergii is increased. The abundance of probiotics in the water body and the immune gene expression of the macrobrachium rosenbergii are obviously improved.

The application discloses a kind of high-activity mysis of macrobrachium rosenbergii screening method, belong to aquaculture technical field.The method includes: pre-treatment of immersion washing, using 100 mesh nylon net to catch I stage mysis, stirring treatment in the first immersion washing container containing 100ppm formaldehyde and 6 ‰ brackish water;First light source induction screening, using 580-595nm, 1500-2000lux LED light source induction above second immersion washing container, and high-activity I stage larvae that float up are collected in batches;Disinfection treatment, after soaking with 20-30ppm povidone iodine, rinse;Again light source induction screening, after the larvae are developed into II stage after constant temperature cultivation at 28-30 DEG C for 24 hours, again light source induction is carried out and high-activity II stage larvae that float up are collected in batches;Finally, concentrated cultivation is carried out.The application realizes the high-efficiency, standardization sorting of high-activity mysis by the combination of specific wavelength light source induction and twice screening before and after key development period, and optimizes immersion washing, disinfection and cultivation parameters, significantly improves the quality of seedling and the survival rate of seedling.

The present application belongs to the field of aquaculture, and particularly relates to a large-specification red paste Macrobrachium rosenbergii breeding method, which comprises the following steps: S1, seedling marking: seedlings are raised in a greenhouse, a pond, an adjustable feed throwing device and a networked automatic feed checking table are arranged in the greenhouse; the feed feeding amount of the adjustable feed throwing device is adjusted through feedback of the networked automatic feed checking table; water quality is adjusted by using micro-ecological preparation and bottom modification preparation; S2, initial screening of male and female: when the marking time is 45-50 days and the average specification reaches 1800-2000 tails / kg, initial screening of male and female is performed to reduce the density of the Macrobrachium rosenbergii in the greenhouse; S3, outdoor pond raising; S4, red paste fattening: female Macrobrachium rosenbergii are fattened with red paste, nutrition fattening feed is fed on Monday, Wednesday and Friday every week, snail meat, carrots and corn are used to replace part of the feed in turn, the replacement proportion is about 50%-60%, normal feed is fed at other times, the fattening period is 4-6 weeks, and the Macrobrachium rosenbergii are caught in late September to early October.

The invention provides a method for incubating larvae of macrobrachium rosenbergii by utilizing underground well water, which comprises the following steps: removing calcium and magnesium ions in the underground well water by adopting a chemical precipitation method to prepare treated well water; mixing the treated well water with external river water to form basic seedling culture water; basic seedling culture water is added into the seedling culture pond, then quicklime and bleaching powder are added, and the basic seedling culture water is disinfected; artificial salt is added into the disinfected basic seedling culture water, and artificial seawater is prepared; larvae of macrobrachium rosenbergii are stocked in artificial seawater, and breeding production is carried out. According to the method, through systematic integration of cooperative utilization of underground well water and external river water, a seasonal dynamic disinfection strategy and multi-dimensional seedling raising environment accurate regulation and control, the incubation effect of macrobrachium rosenbergii larvae is remarkably improved, and the physicochemical indexes of water quality are better, the growth of the larvae is faster, the metamorphosis development is faster, and the survival rate is higher.

The invention relates to the technical field of pomacea canaliculata resourceful treatment and aquaculture, in particular to a macrobrachium rosenbergii breeding method based on pomacea canaliculata resourceful treatment. The method comprises the following steps: sequentially carrying out multi-stage purification on captured ampullaria gigas through temporary culture emptying, circulating micro-flowing water washing and EM bacterial liquid soaking, then integrally crushing the ampullaria gigas into ampullaria gigas meat paste, adding table salt and / or organic acid, and then mixing the ampullaria gigas meat paste with a vitamin-rich plant raw material and a vitamin mineral premix according to a ratio; and the soft pomacea canaliculata block bait which is not easy to foam and scatter is formed by extrusion or compression molding in cooperation with a hydrophilic gel binder. In a breeding pond of the macrobrachium rosenbergii, the soft block bait is matched with compound feed for the parent macrobrachium rosenbergii for precise feeding according to a limited proportion and a limited period of time, and the feeding amount and water change are dynamically adjusted in combination with water quality indexes, so that the gonad development and reproductive performance of the macrobrachium rosenbergii are improved, the water quality is stabilized, and the bait cost is reduced.

The present application belongs to the field of aquaculture, and particularly relates to a feeding supervision method for Macrobrachium rosenbergii. The feeding supervision system is characterized by comprising an adjustable feed throwing device and a networked automatic feed checking table. The adjustable feed throwing device comprises a feed throwing box, a wind feeding mechanism and a suspended throwing mechanism. The networked automatic feed checking table is arranged at the suspended throwing mechanism and comprises a lifting mechanism, a feed checking table, a weighing mechanism, a shooting mechanism, a networked module and a water quality detection net.

The invention discloses a compound probiotic preparation for breeding macrobrachium rosenbergii as well as a preparation method and application of the compound probiotic preparation, and relates to the technical field of microorganisms, the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski, and the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski, and the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski. Wherein the preservation number of the lactococcus format is CGMCC (China General Microbiological Culture Collection Center) No.31024. According to the physiological requirements of different development stages of the Z1-Z4 stage and the Z5-P1 stage of larvae, 2: 1 and 2: 1: 1 bacterial strains are adopted for staged accurate addition respectively, meanwhile, artificial seawater of a specific formula is matched, baits such as fairy shrimps and egg custard are quantitatively fed according to the development stage, key water quality indexes such as dissolved oxygen, ammonia nitrogen and nitrite in a water body are strictly regulated and controlled, and the quality of the larvae is improved. A green seedling raising technical system integrating probiotic micro-ecological regulation and control, precise nutrition supply and water quality management is formed, and finally the effects of improving the survival rate of larvae, reducing the metamorphosis rate, promoting growth and development and replacing antibiotics for use are achieved.

The utility model provides a grading screen for breeding macrobrachium rosenbergii larvae, which comprises a base, an arch-shaped mounting frame is fixedly mounted at the top of the base, a grading screening mechanism for the macrobrachium rosenbergii larvae is arranged on the inner side of the arch-shaped mounting frame, a water collecting tank is fixedly mounted at the top of the base, and a collecting tank is arranged at the top of the water collecting tank. And the macrobrachium rosenbergii larva grading screening mechanism comprises three screening grooves which are vertically placed, nylon filter screens are fixedly installed at the bottoms of the screening grooves, inserting grooves are formed in the right sides of the screening grooves, baffles are arranged in the inserting grooves in a penetrating and inserting mode, and two circular connecting rings are fixedly installed on the inner side of an arch-shaped installation frame. The base is arranged to achieve installation of the water collecting tank, the water collecting tank is arranged to collect and discharge water, and the macrobrachium rosenbergii larva grading screening mechanism is arranged to achieve grading filtering of macrobrachium rosenbergii larva. Further, larvae of macrobrachium rosenbergii with different sizes are separated, so that the survival rate during subsequent culture can be increased.

This invention discloses a method for co-culturing giant freshwater prawns (Macrobrachium rosenbergii) with shrimp, grass, and fish based on probiotic regulation, and its application. This invention constructs a three-dimensional ecological system with shrimp as the main body, grass as the protector, and fish as a supplement. Elodea and water spinach purify the water, silver carp and crucian carp decompose uneaten feed and feces, and a compound microbial preparation of Lactococcus gasseri C6a2 regulates water quality, while a biological bottom conditioner improves the bottom sediment, achieving precise ecological regulation of the aquaculture environment. This invention does not rely on chemical agents, effectively inhibits ammonia nitrogen accumulation, reduces disease incidence, and significantly improves the survival rate and size of giant freshwater prawns, achieving a win-win situation for both ecological and economic benefits.

The present application belongs to the field of biotechnology and genomics, and particularly relates to a method and system for determining the chromosome base number of Macrobrachium rosenbergii based on multi-omics joint analysis. The method obtains de novo assembly sequencing data and Hi-C sequencing data, generates a chromosome-level candidate assembly without presetting the number of chromosomes by using Hi-C interaction signals after primary assembly, and performs whole-genome collinearity alignment with no less than two published reference genomes; in combination with quality constraints such as collinearity continuity, Hi-C boundary characteristics and BUSCO / LAI, the candidate chromosome boundary is comprehensively judged and iteratively converged, and finally the chromosome base number and reviewable evidence chain are output. The embodiments show that the present application can identify and correct the number redundancy caused by over-splitting of the reference genome, determine the base number of Macrobrachium rosenbergii as n=57 (2n=114), and improve the objectivity and reliability of base number determination.

The invention discloses a macrobrachium rosenbergii freezing preservative as well as a use method and application thereof, and belongs to the technical field of shrimp preservatives. According to the macrobrachium rosenbergii freezing preservative provided by the invention, all the components have no potential safety hazard to human health and ecological environment; the macrobrachium rosenbergii freezing fresh-keeping agent is prepared by creatively utilizing daily seasonings and oligopeptides in cells as effective components, tests prove that the prepared fresh-keeping agent is combined with a specific using method, the fresh-keeping effect on the macrobrachium rosenbergii is very excellent, no obvious cellular content flows out after the macrobrachium rosenbergii is frozen at-20 DEG C for 6 months, the boiling quality is relatively good, and the macrobrachium rosenbergii freezing fresh-keeping agent is suitable for large-scale popularization and application. The shrimp is bright in color, rich in shrimp flavor, good in meat plumpness, appropriate in fiber compactness and hardness and free of obvious salty taste; the provided macrobrachium rosenbergii freezing fresh-keeping agent is simple in preparation method, easy to operate, wide in raw material source, high in cost performance and suitable for freezing fresh-keeping of macrobrachium rosenbergii.

The invention discloses dsRNA (double-stranded ribonucleic acid) of a targeted IPV (infectious precocity virus) NS1 gene and application of the dsRNA. The dsRNA disclosed by the invention consists of a positive-sense strand of which the nucleotide sequence is as shown in SEQ ID NO.1 and an antisense strand of which the nucleotide sequence is as shown in SEQ ID NO.2. The dsRNA is injected into the macrobrachium rosenbergii infected with the IPV virus, so that the load of the IPV virus can be obviously reduced by utilizing the dsRNA, and the growth inhibition of the IPV virus on the macrobrachium rosenbergii is relieved. On the basis, the dsRNA or a biological material capable of expressing the dsRNA, such as a cell or a strain capable of expressing the dsRNA, can be used for preparing a reagent for inhibiting the IPV virus, so that viral diseases caused by IPV virus infection are prevented and treated. The composition is beneficial to prevention and treatment of IPV virus related diseases, and promotes the long and stable development of the macrobrachium rosenbergii breeding industry.

The present invention discloses a water purification treatment device and treatment method for the water used in the breeding of Macrobrachium rosenbergii, belonging to the field of shrimp seedling breeding. A water purification treatment device for the water used in the breeding of Macrobrachium rosenbergii includes a support frame, and also includes: a treatment tank and a sewage pump, both fixedly connected to the support frame. Among them, a water suction pipe and a drain pipe are respectively fixedly connected to the output end and the input end of the sewage pump, the end of the drain pipe extends to the inner top of the treatment tank, and a plurality of branch pipes distributed circumferentially are fixedly connected to the lower end of the treatment tank; a storage tank with an end cover installed at the top is fixedly installed at the upper end of the treatment tank. Among them, a thin pipe extending into the treatment tank is fixedly connected to the lower end of the storage tank, and a stirring assembly is arranged in the storage tank; the present invention can improve the uniformity of the sewage and bleaching powder, thereby improving the treatment effect on the sewage, and the bleaching powder is not likely to be scattered in the air to pollute the surrounding environment.

The utility model relates to the technical field of aquaculture equipment, in particular to a macrobrachium rosenbergii greenhouse pool oxygenation device which comprises an oxygenation device body, the oxygenation device body comprises an air source, and the air source is connected with an oxygenation pipe through a pipeline; the supporting assembly comprises a supporting frame and a floating assembly; and the protection assembly comprises a fixed part and a movable part. According to the utility model, the supporting assembly is arranged, and the oxygen-increasing pipe is too high by the supporting frame, so that the oxygen-increasing pipe is prevented from sinking into sludge and the service life is prolonged; the protection assembly is arranged, the sealing cover and the floating assembly are connected through the connecting plate, when the oxygenation pipe is ventilated, the buoy ascends, the sealing cover can be driven to ascend, air is conveniently discharged by the air stone, bubbles are generated, and oxygenation is achieved; and when the oxygenation pipe is not ventilated, the buoy descends to drive the sealing cover to descend and is matched with the bottom plate to wrap the bubble stone to prevent the bubble stone from being blocked.

The invention belongs to the field of biotechnology and genomics, and particularly relates to a method and system for determining the chromosome cardinal number of macrobrachium rosenbergii based on multi-omics conjoint analysis. According to the method, de novo assembly sequencing data and Hi-C sequencing data are obtained, after primary assembly is completed, chromosome-level candidate assemblies are generated under the condition that the number of chromosomes is not preset through Hi-C interaction signals, and whole-genome collinearity comparison is carried out on the chromosome-level candidate assemblies and no less than two disclosed reference genomes; and in combination with quality constraints such as collinearity continuity, Hi-C boundary features and BUSCO / LAI, carrying out comprehensive decision and iterative convergence on candidate chromosome boundaries, and finally outputting chromosome cardinal numbers and reviewable evidence chains. The embodiment of the invention shows that the method can identify and correct the number redundancy caused by the over-resolution of the reference genome, determines the cardinal number of macrobrachium rosenbergii as n = 57 (2n = 114), and improves the objectivity and reliability of cardinal number determination.

The invention discloses a method for detecting nitrofuran metabolites in macrobrachium rosenbergii based on UPLC-MS / MS (Ultra Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) and application of the method, and belongs to the technical field of safety evaluation and detection of aquatic products. According to the method, a macrobrachium rosenbergii sample is pretreated by utilizing a QuECHERS method, nitrofuran metabolites are extracted, and the method comprises the steps of adding acidified acetonitrile for extraction, adding ammonium acetate and sodium chloride for water removal, adding a dispersed solid extraction column for reducing a matrix effect and the like. After the treatment by the QuECHERS method disclosed by the invention, the obtained nitrofuran metabolite does not need to be subjected to a derivatization procedure, and an HILIC hydrophilic chromatographic column can be directly used for carrying out liquid chromatography-mass spectrometry analysis in a positive ion mode so as to detect the nitrofuran metabolite such as semicarbazide and the like. The detection method provided by the invention has the advantages of high sensitivity, strong accuracy, wide applicability and the like, and compared with the traditional detection method, the reagent waste can be avoided, and a large amount of time can be saved.

The present application relates to the technical field of phenotype measurement in aquatic genetic breeding, and particularly relates to a high-throughput phenotype measurement method and system for small-sized Macrobrachium rosenbergii. In the measurement box, a dark box environment is constructed by a white shooting bottom plate, dark light-absorbing inner walls and an LED surface array light source, combined with preview graph rough segmentation and local gray histogram analysis, to adaptively adjust the light to make the shrimp body and the background form a stable bimodal gray distribution. On this basis, a segmentation-key point joint deep learning model is used to simultaneously output the shrimp body mask and standard key points, and the topological consistency loss constraint of the skeleton side length ratio and the included angle is introduced in the training to significantly reduce the key point mislabeling and drift caused by transparent body color. The present application realizes the rapid, non-contact, high-precision and high-throughput determination of the phenotype parameters and weight of small-sized Macrobrachium rosenbergii individuals, and provides a high-quality structured phenotype database for genetic evaluation and multi-trait joint selection.

The application discloses a method for predicting and breeding Macrobrachium rosenbergii chela ratio based on phenomics analysis, comprising the following steps: selecting a plurality of Macrobrachium rosenbergii for multi-generation breeding, recording pedigree data of each individual, and constructing a Macrobrachium rosenbergii breeding database; extracting the phenotypic characteristics of Macrobrachium rosenbergii at different breeding stages, analyzing the relationship between the growth index and the chela ratio, and evaluating the genetic force to construct a Macrobrachium rosenbergii phenomics network; analyzing the growth path of Macrobrachium rosenbergii, identifying abnormal events during breeding, and generating a Macrobrachium rosenbergii growth trend network; constructing a Macrobrachium rosenbergii phenotype knowledge graph, and constructing a Macrobrachium rosenbergii chela ratio prediction model; collecting early phenotype data of the Macrobrachium rosenbergii population to be bred, making breeding decisions, and recommending breeding paths for the breeding individuals by using the Macrobrachium rosenbergii phenotype knowledge graph. Whether the breeding path is deviated in the breeding process is analyzed at the same time, and a correction scheme is formulated and pushed, so that the breeding cycle of Macrobrachium rosenbergii is shortened, and the accuracy and efficiency of breeding are improved.

The invention discloses a macrobrachium rosenbergii fry breeding method based on concentrated natural seawater, and relates to the technical field of aquaculture breeding, and the method comprises the following steps: S1, selecting surface seawater of a sea area of the northern bay of Guangxi, and sequentially carrying out pretreatment, concentration treatment and retreatment to obtain concentrated seawater; the pretreatment comprises precipitation, precise filtration and sterilization; the concentration treatment adopts a reverse osmosis method or is combined with a double-effect evaporation method, and the seawater is concentrated to 1 / 3-1 / 5 of the original volume; the retreatment comprises the following steps: adjusting the pH value to 7.8-8.2, adding an EDTA-2Na chelating agent, and carrying out precise filtration; s2, performing differential precipitation, three-stage gradient filtration and compatibility pre-detection on the selected water source to ensure that the turbidity of dilution water is less than or equal to 3NTU, the pH is 7.5-8.0, the ammonia nitrogen is less than or equal to 0.02 mg / L, and the residual chlorine is equal to 0mg / L. According to the method, through whole-course water quality management and staged accurate feeding, efficient, stable and green breeding from larvae to juvenile shrimps is achieved.