Method for sex induction of hermaphrodite shrimps

A technique for body shrimp, male and female, which is applied in the field of sex regulation of aquaculture animals, can solve the problems of difficulty in obtaining male gland samples, and difficulty in large-scale industrial application of trace extracts, and achieves the effect of clear implementation steps.

Inactive Publication Date: 2015-07-01
ZHEJIANG WANLI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is not easy to obtain androgenic gland samples, and it is not easy to carry out large-scale industrial application of a small amount of extract

Method used

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  • Method for sex induction of hermaphrodite shrimps
  • Method for sex induction of hermaphrodite shrimps
  • Method for sex induction of hermaphrodite shrimps

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Step (1) double-stranded RNA sample preparation

[0031] According to the cDNA sequence of the screened sex gene, design forward and reverse primers with restriction sites, RNAi-PF: 5′-GC TCTAGA GTGTGTGTTGTTCTGCTCACTCGT-3′) (the underline is the XbaI restriction site SEQ ID NO: 2) and RNAi-PR (SEQ ID NO: 3: 5′-C GAGCTC GCAGCATTCCTCCCTTGGACTTCT-3') (the underline is the SacI restriction site), which is used for PCR reaction amplification to obtain the target gene sequence as follows:

[0032] Gtgtgtgttgttctgctcactcgtagcatcgcttctccctcaaccttcttcgagctatgagatcgaatgcctctccgttgactttgactgcggcgacataacgaacacccttgcctccgtctgcctgagacacaacaactacatcaacccaggacccacctacgtttccaaagagcgacgatctgctgacatctataccgttccttctacgaagtctccatcgctcgcccacccgagagctacccacttgaccatggctgacgaagaaactcagaaggtatctaaggtggaggaggagattcagcacatgacgctgagcagggaagaagcgaacaatatgctgcattcgaagcgtcgcttccggagggacagcgtgaggagaagtccaagggaggaatgctgc(SEQ ID NO:1)

[0033] The double-stranded RNA sample is synthesized and prepared...

Embodiment 2

[0043] Macrobrachium rosenbergii ampullate peptide gene (TAP) is another androgenic gland-specific gene that was screened from the androgenic gland cDNA subtraction library of Macrobrachium rosenbergii. The full sequence of the gene can be obtained from the gene bank (sequence number FJ595507) .

[0044] In this example, the ampullate peptide gene will be taken as an example to describe in detail how to achieve efficient silencing of the target gene in Macrobrachium prawns and conduct sex induction in hermaphrodites through RNA interference technology and Macrobrachium rosenbergii animal experiments.

[0045] The present invention will be described in detail below in conjunction with the examples.

[0046] Step (1) double-stranded RNA sample preparation

[0047] According to the cDNA sequence of the ampulla peptide gene, design forward primer and reverse primer with restriction site, RNAi-PF (SEQ ID NO:4:5'-GC TCTAGA CTTTTCTTGTTGATGGCACT-3') (the underline is the XbaI restr...

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Abstract

The invention aims to provide a method for sex induction of hermaphrodite shrimps. According to the method, a target gene in macrobrachium is silenced by using an RNA interference technology to realize sex induction of macrobrachium rosenbergii, wherein the nucleotide sequence of the target gene is SEQ ID NO:1. By virtue of the induction technology provided by the invention, a macrobrachium individual has sex reversion and becomes hermaphrodite macrobrachium rosenbergii, and the developments of a female reproductive system and a male reproductive system are realized in the body of the hermaphrodite macrobrachium rosenbergii, so that a set of effective macrobrachium rosenbergii sex induction technology is established. Meanwhile, by using the hermaphrodite shrimps cultured by the method provided by the invention, a good scientific research material and an animal sample can be provided for sex determination, sex differentiation and researches on reproduction and fertilization of shrimps and crabs.

Description

technical field [0001] The invention belongs to sex regulation technology of aquaculture animals, in particular to a sex induction technology of shrimps, in particular to a technology of induction of hermaphroditic Macrobrachium rosenbergii with both female reproductive system and male reproductive system. Background technique [0002] Hermaphroditism refers to the phenomenon that both female and male traits are obvious in one animal body, while dioeciousness refers to the phenomenon that the male and female reproductive organs of the same animal are born in different individuals. Hermaphroditism in nature mostly occurs in lower animals. Macrobrachium rosenbergii (Macrobrachium rosenbergii) is a large freshwater prawn, which has the advantages of fast growth, wide appetite, good meat quality and nutritional content, and short breeding period. Macrobrachium rosenbergii in nature is a dioecious organism, and its female and male reproductive organs exist in female and male ind...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12N15/12A01K67/033
Inventor 马文明曹荣荣林挺钱国英俞炎琴杨劲树杨帆杨卫军
Owner ZHEJIANG WANLI UNIV
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