49results about How to "Good secretion effect" patented technology

The invention relates to the technical field of cell culture, and particularly relates to a culture method of mesenchymal stem cells. In the culture method, a serum-free culture medium optimized by the invention is used for culturing the mesenchymal stem cells. In addition, the invention further provides a better culture method for 3D culture. The cells obtained by the culture method disclosed bythe invention are strong in proliferation capacity, intact in shape and strong in secretion capacity, and are in accord with the international quality control standards of the mesenchymal stem cells.Meanwhile, according to the culture method disclosed by the invention, a large number of mesenchymal stem cells (particularly human umbilical cord mesenchymal stem cells) can be obtained by amplifyinga small number of mesenchymal stem cells under the conditions of small space occupation, less culture medium consumption, simpler and more convenient operation and greatly reduced workload; and a solid technical scheme and a theoretical basis are provided for obtaining a large number of high-quality mesenchymal stem cells (especially human umbilical cord mesenchymal stem cells) and secretions thereof in the clinical field.

The invention discloses a feed additive for postpartum sows and a production method of the feed additive. The feed additive is characterized by mainly comprising the following raw materials in parts by weight: 15-20 parts of heartleaf houttuynia herb, 15-20 parts of haws, 15-20 parts of Chinese flowering quinces, 10-15 parts of radix scutellariae, 10-15 parts of dandelions, 5-10 parts of forsythia suspensa, 5-10 parts of ligusticum wallichii, 2-4 parts of fructus gardeniae, 2-4 parts of fresh ginger, 1-3 parts of siraitia grosvenorii, 0.1-0.5 part of sea salt and 0.1-0.5 part of yellow wine, and the feed additive is liquid or solid powder. Through scientific concerted application, the feed additive for the postpartum sows, disclosed by the invention, has favorable effects of preventing and treating the diseases that the postpartum sows often suffer from, such as mammitis, metritis, metritis-mastistis-agalactia (MMA), pospartum constipation and postpartum aphagia, is free from toxic or side effects, and is low in price; the feed additive also has the characteristics of clearing heat, removing toxicity, stimulating appetite, being capable of obviously improving immunity and the like.

The invention discloses a lysozyme mutant with improved specific activity, belonging to the technical field of bioengineering. The invention discloses the human lysozyme mutant which is obtained by adding hydrophobic oligopeptide Val-Ile-Pro-Leu-Phe to the C end of human lysozyme through genetic engineering. The specific activity of the human lysozyme mutant is improved by 126%. The invention alsodiscloses a recombinant plasmid containing a mutant human lysozyme gene, and a recombinant genetically-engineered pichia pastoris strain which is obtained by transforming pichia pastoris with the recombinant plasmid and capable of efficiently expressing the human lysozyme mutant. The obtained human lysozyme mutant has the characteristics of high specific activity and simple preparation, has potential clinical application value, and also has wide application in feed and food industries.

The invention relates to a preparation method of a rooting and synergizing organic fertilizer. The preparation method comprises the steps of adding a rooting agent and a wetting emulsifier into a stirring reactor containing a synergist according to a certain mass ratio; uniformly stirring, starting to stir and slowly adding a thickening agent at the temperature of 20 to 85 DEG C; stirring until the thickening agent is completely dissolved; cooling to the room temperature so as to obtain a synergist with a rooting function; adding the synergist with the rooting function in a fertilizer during an organic fertilizer production process, and obtaining the rooting and synergizing organic fertilizer. According to the preparation method of the rooting and synergizing organic fertilizer provided by the invention, the fertilizer utilization ratio can be remarkably improved and the rooting effect is good.

The invention relates to a multifunctional meridian physical factor integrated therapeutic apparatus which integrates an electrotherapy funtion, a magnet therapy function, a phototherapy function and a thermal therapy function. The meridian physical factor integrated therapeutic apparatus comprises a host computer and two sensors with the same functions, more than two output electrodes are arranged on the front face of each sensor, an infrared heating component and a traditional Chinese medicine plate are arranged in a cavity body at the bottom of each sensor, each infrared heating component is arranged on one traditional Chinese medicine plate, a control panel is arranged on the host computer, an integrated control circuit board is arranged inside the host computer, and the host computer is connected with the two sensors through wires. The meridian physical factor integrated therapeutic apparatus has the advantages that the therapeutic apparatus effectively integrates effective physical factors including electricity, light, magnetism and heat in a physiotherapy to act on meridians, acupuncture points and viscera portions and rapidly enables vital energy and blood pathways inside and outside the human body to be communicated by adjusting vital energy and blood, dredging the channels and stimulating the acupuncture points, therefore, the therapeutic apparatus plays a role in preventing or curing a disease and conducting body building, and meanwhile the therapeutic apparatus is simple in structure, convenient and rapid to use, and obvious in therapeutic effect.

The invention provides a series of instant-brewing white-fungus fruit-juice beverage paste and a preparation method thereof. The series of instant-brewing white-fungus fruit-juice beverage paste are prepared from the following raw materials: 20-60 parts fresh white fungi, 200-300 parts of fruits, 5-15 parts of crystal sugar, 4-7 parts of honey, 100-150 parts of sucrose, 1-2 parts of sodium dihydrogen phosphate, 0.1-0.8 part of citric acid, 1.5-2 parts of D-sodium isoascorbate, 0.01-0.02 part of pectinase, 0.05-0.1 part of cellulase, 0.02-0.04 part of glucose oxidase, and 1-2 parts of edible essences. For the white-fungus fruit-juice beverage paste disclosed by the embodiments of the invention, specific raw materials are combined, and through addition of additives including the citric acidwith the effects of protecting color and stabilizing nutrients, addition of compound enzymes, and synergistic compatibility of the raw materials, the effect of convenient and instant eating of the white-fungus fruit-juice beverage paste is achieved.

The present invention provides a series of white fungus jam with health caring and preserving functions and a preparation method thereof. The series of white fungus jam comprises the following raw materials: 20-60 parts of fresh white fungus, 200-300 parts of fruit products, 5-10 parts of Chinese wolfberry fruits, 5-10 parts of longan, 5-10 parts of red dates, 5-15 parts of crystal sugar, 4-7 parts of honey, 100-150 parts of sucrose, 1-2 parts of sodium dihydrogen phosphate, 0.1-0.8 part of citric acid, 1.5-2 parts of sodium D-isoascorbate, 0.01-0.02 part of pectinase, 0.05-0.1 part of cellulase, 0.02-0.04 part of glucose oxidase and 2-5 parts of lemon juice. The series of white fungus jam of the embodiment is prepared by combining the specific raw materials; additives, such as the citricacid, having functions of protecting color and stabilizing nutrients are added; the compound enzyme substances are added; the raw materials are combined to have synergism; and the series of white fungus jam achieves the health caring and preserving functions.

The invention relates to a traditional Chinese medicine composition for treating chronic prostatitis and a preparation method thereof. The traditional Chinese medicine composition is prepared from thefollowing raw materials in parts by weight: 8 to 12 parts of cortex eucommiae chlorogenic acid, 6 to 10 parts of cortex cinnamomi polyphenol, 13 to 17 parts of oyster peptide, 8 to 12 parts of maca,8 to 12 parts of cordyceps militaris, 13 to 17 parts of silk peptide, 2 to 6 parts of herba abelmoschi esculenti, 2 to 6 parts of cortex pausinystaliae macroceras, 2 to 6 parts of catuaba, 2 to 6 parts of herba epimedii, 2 to 6 parts of herba gynostemmatis, 2 to 6 parts of herba rhodiolae, 2 to 6 parts of radix salviae miltiorrhizae and 2 to 6 parts of semen persicae. The traditional Chinese medicine composition disclosed by the invention is simple in preparation and remarkable in curative effect, has no side effect, is safe and effective, is low in cost and is suitable for oral administration.

The invention discloses an endometrial stem cell preparation for treating erectile disorder and a preparation method thereof. Stem cells are pluripotent cells with self-replication capacity, and the stem cells can be differentiated into multiple functional cells under certain conditions. Through stem cell transplantation, damaged cells are replaced by direct differentiation or a large number of cell factors are secreted in a paracrine mode, so that the treatment process is simplified, which is different from the mode that a medicine only aims at specific molecules or signal channels, and therefore, endometrial stem cells which are tissue repair cells with vascular regeneration and repair activity are cultured in vitro. By adopting a micro-injection planting technology, active cells are planted in a male cavernous body in a targeted manner, so that on one hand, the generation of new small vessels can be promoted, and on the other hand, damaged and blocked arterioles can be repaired, themicrocirculation of blood vessels is improved, and the blood supply speed and blood supply quantity of dorsal arteries to the cavernous body are increased; and a safe and effective treatment means isprovided for treating erectile disorder.

The invention discloses a separation and purification method of recombinant parathyroid hormone (PTH), which comprises the following steps: 1) bacterium crushing and inclusion body treatment: carryingout high-pressure homogenization and bacterium crushing, carrying out high-speed centrifugation after bacterium crushing to collect precipitates, carrying out resuspension washing on the precipitates, and mixing and redissolving the precipitates with a redissolved solution; 2) affinity chromatography; wherein a filler is selected from one of Ni-NTA, GST Sepharose and MBP Sepharose; 3) carrying out enzyme digestion and renaturation on a column; 4) carrying out cation exchange chromatography and/or hydrophobic chromatography, and (5) desalting. According to the present invention, through the method, the PTH (1-34) polypeptide with biological activity can be efficiently extracted from an inclusion body so as to make the large-scale industrial production of the PTH (1-34) possible.

The present invention discloses one kind of pinellia tuber extract and its preparation process, medicine composition and use. The pinellia tuber extract is prepared with fresh pinellia tuber, and through air dying, crushing to operate pinellia tuber meal, heat reflux or percolating with organic solvent and/or water for 2-4 times to obtain the extracted liquid, concentrating and drying the extract liquid. The pinellia tuber extract has excellent effects of relieving cough, eliminating phlegm, stopping vomiting, diminishing inflammation, etc. and may be used in treating pneumosilicosis, cough, gastric ulcer, arrhythmia, tumor and other diseases.

The invention belongs to the field of biological medicine, and relates to an anti-TIGIT antibody and application thereof. Specifically, the invention relates to an anti-TIGIT antibody or an antigen binding fragment thereof, the antibody comprises a heavy chain variable region and a light chain variable region, the amino acid sequences of HCDR1 to HCDR3 of the heavy chain variable region are respectively shown as SEQ ID NOs: 3-5, and the amino acid sequences of LCDR1 to LCDR3 of the light chain variable region are respectively shown as SEQ ID NOs: 8-10; and according to an EU numbering system, the heavy chain constant region comprises mutation such as L234A and L235A. The antibody disclosed by the invention can be effectively combined with TIGIT, has small toxic and side effects and has the potential of being applied to tumor prevention and treatment.

The invention discloses a traditional Chinese medicine immunity enhancer suitable for poultry and a preparation method thereof. The traditional Chinese medicine immunity enhancer is prepared by combining radix astragali seu hedysari, radix codonopsis, ginkgo fruit pulp, herba epimedii and radix glycyrrhizae. The public health problems of drug residues in animal foods, bacterial drug resistance and toxic and side effects at different degrees on the animal body, caused by long-time and high-dose application of antibiotics and chemical drugs, can be overcome. The traditional Chinese medicine immunity enhancer comprises traditional Chinese medicines with functions of strengthening body resistance or eliminating evil; the proliferation of poultry splenic lymphocyte, synthesis of antibody, secretion of cell factors and immune organ index can be enhanced; the immunity of the poultry body can be enhanced; the healthy development of poultry breeding can be facilitated.

The invention relates to a preparation method of a rooting synergist major-element liquid water-soluble fertilizer. The preparation method comprises the steps that a rooting agent and a wetting emulsifying agent are added into a stirring kettle containing a rooting synergist according to a certain mass ratio, after the materials are stirred to be uniform, stirring is started and a thickening agent is slowly added at the temperature of 20 DEG C to 85 DEG C, stirring is conducted till the thickening agent is completely dissolved, the mixture is cooled to room temperature, a rooting synergist with the rooting effect is obtained, the rooting synergist with the rooting effect is added into a major-element liquid water-soluble fertilizer, the materials are stirred to be uniform, and the rooting synergist major-element liquid water-soluble fertilizer is obtained. According to the preparation method, growth of a plant root system is stimulated while the fertilizer utilization ratio is increased, and the purpose of maximally increasing the yield is achieved by combining internal causes and external causes which influence plant nutrition absorption.

The invention discloses a method for acquisition of a sugar-reducing medicine and the sugar-reducing medicine, and relates to the technical field of the medical treatment and public health. The method includes steps of through inhibiting inflammation, improving the insulin secretion capacity and the insulin resistance capacity of an early-stage INS-1 pancreas islet Beta cell line and a rodent experimental animal, and realizing the reduction of blood sugar, specifically using a pharmaceutical containing a p38 MAPK inhibitor ingredient SB203580 to realize. The provided sugar-reducing medicine is capable of preparing a pharmaceutical for nursing, delaying and/or treating diabetes or future application in a health care product. The p38 MAPK inhibitor SB203580 is capable of remarkably improving the insulin secretion capacity and the insulin resistance capacity of diabetes patient pancreas islet Beta cells, reducing the blood sugar level of the diabetes patient, and delaying and improving the development of the diabetes through inhibiting the inflammation, and has extensive application prospect in the treatment and nursing of the diabetes.

The invention relates to the technical field of breeding, and particularly relates to a breeding method of Luhua chickens. The breeding method comprises the following steps: feeding Luhua chickens atthe age of 2-3 weeks by using prepared feed, wherein the feeding is carried out 3 times per day, 100-300g of the feed is fed to each chicken for each time, and the chickens are freely fed until the chickens are full. The feed comprises the following components: 50-60% of corn meal, 10-15% of aloe dry powder, 8-15% of sesame seed meal, 5-8% of fermented egg shell, 2-5% of corn dried distiller's grains, 1-3% of table salt, 3-5% of calcium hydrogen phosphate and 2-5% of a mixed material of traditional Chinese medicine extracts. The breeding method provided by the invention has the advantages thatthrough the timely feeding by using the feed provided by the application, the growth rate can be improved, and a series of complicating diseases caused by the increase of blood fat can be well controlled; in addition, the feed has a good insecticidal effect, and the phenomenon that the Luhua chickens are poisoned by freely eating harmful substances is avoided, so that the survival rate of the Luhua chickens is improved, and further the purpose of increasing the income is achieved.

The invention discloses female animal estrus stimulating superfine powder and a method for preparing the same. Preparation raw materials for the female animal estrus stimulating superfine powder include herba epimedii, (wine-quenched) actinolite, radix angelicae sinensis, rhizoma cyperi, herba leonuri, fructus corni, liriope spicata, panax japlcus var and rhizoma polygonati odorati. The female animal estrus stimulating superfine powder and the method have the advantages that the problems of low female animal estrus rate and parturition rate, persistent failure to conception and high abortion probability can be effectively solved by the aid of the female animal estrus stimulating superfine powder and the method, and growth and development of female animals further can be promoted; impurities in the various raw materials can be effectively removed by the aid of the specific method, and secondary pollution on the raw materials can be prevented; the quality and the uniformity of coarse powder can be guaranteed; the efficacy can be easily realized; the female animal estrus stimulating superfine powder can be easily absorbed; estrus stimulating powder can be conveniently stored and usedin later periods; the female animal estrus stimulating superfine powder can be conveniently stirred in feed and can be conveniently mixed with drinking water during administration in the later periods, and the medicine sensitivity of the female animals can be effectively lowered.

The invention discloses a traditional Chinese medicine capsule for treating perimenopausal syndrome. The capsule is prepared from the active components in parts by weight: 70-85 parts of human placenta, 70-75 parts of radix pseudostellariae, 139-145 parts of radix paeoniae alba (fried), 139-160 parts of spina date seeds (fried), 139-145 parts of poria with hostwood, 139-150 parts of oyster (calcined), 139-160 parts of ossa draconis (calcined), 139-145 parts of dried rehamnnia root, 139-160 parts of vine of multiflower knotweed, 139-150 parts of lily, 60-70 parts of Chinese honeylocust spine, 80-90 parts of sargent gloryvine, 60-70 parts of peach seeds, 139-150 parts of red flower, 139-145 parts of prepared rehmannia root, 100-110 parts of the seed of cowherb, 70-75 parts of garden balsam stem, 139-145 parts of herba epimedii, 139-150 parts of dried tangerine or orange peel, 80-90 parts of curcuma zedoary, 100-110 parts of artemisia anomala, 20-28 parts of trigone and 70-75 parts of salvia chinensis. The traditional Chinese medicine capsule is simple in preparation process, accessible in raw material, low in cost, good in effect on kidney yin-yang deficiency type perimenopausal syndrome, good in effect and high in recovery rate.