12 results about "Splenic lymphocyte" patented technology

The application discloses a use of Ejiao Qianggu oral liquid in regulating immunity, and particularly relates to an improving effect of the Ejiao Qianggu oral liquid on an immune system, which is realized by the following aspects. On one hand, the Ejiao Qianggu oral liquid promotes proliferation and transformation (mainly early T lymphocytes and part of B lymphocytes) and active functions of spleen lymphocytes; on the other hand, the Ejiao Qianggu oral liquid improves phagocytosis of macrophages. The Ejiao Qianggu oral liquid has the following characteristics: firstly, the Ejiao Qianggu oral liquid acts on early lymphocyte maturation, and has no significant effect on proliferation of peripheral blood lymphocytes; secondly, the Ejiao Qianggu oral liquid can significantly act on immune target organs, thymus and spleen tissues, and does not excessively stimulate the spleen, unlike positive drugs such as colony stimulating factor which can cause significant edema of the spleen; and thirdly, the Ejiao Qianggu oral liquid has a significant recovery effect on body weight, white blood cells, lymphocytes, red blood cells and hemoglobin in blood of patients with low immunity.

This invention belongs to the field of plant extract technology, specifically relating to a plant extract composition for enhancing immunity. By weight, it is composed of the following raw materials: 3-5 parts Astragalus membranaceus extract, 2.5-4 parts Echinacea purpurea extract, and 1.5-3.5 parts Rhodiola rosea extract. This plant extract composition, through the scientific formulation of Astragalus membranaceus, Echinacea purpurea, and Rhodiola rosea, and an enzymatic extraction process, enhances the immunomodulatory effect. The combination of the three extracts increases the secretion of TNF-α and IL-6 by macrophages. Furthermore, the spleen lymphocyte stimulation index (SI) of the three extracts reaches 5.57, and the lymphocyte transformation rate increases from 191% to 457%. These results indicate that this plant extract composition effectively enhances the body's non-specific and cellular immune functions by activating macrophages and promoting T lymphocyte proliferation through multiple pathways, providing more comprehensive immune protection.

The invention relates to a construction method and application of an immune system sheep-derived mouse model, and the construction method comprises the following steps: 1) taking an adaptively cultured severe immunodeficiency mouse, and injecting busulfan to inhibit the hematopoiesis of the mouse; 2) injecting sheep spleen lymphocytes into the body of the pre-treated immunodeficient mouse in an orbital vein manner; 3) after the sheep cells are transplanted in the step 2), detecting the cell proportions of sheep CD45 +, CD4 + and CD8 + in the peripheral blood of the mouse every week so as to detect the sheep CD45 +, CD4 + and CD8 + in the peripheral blood and / or tissues, and determining that the immune system sheep mouse is successfully constructed if the sheep CD45 +, CD4 + and CD8 + can be continuously detected for 13 weeks; (4) injecting and infecting Brucella into the abdominal cavity of the mouse in the step (3) to obtain the Brucella infected sheep-derived mouse model.According to the method, sheep spleen lymphocytes are transplanted into the mouse body to construct the immune system sheep-derived mouse, and a more effective and more convenient animal model is provided for studying diseases such as Brucella taking sheep as a host.

This invention relates to the field of biomedical technology, specifically to a snake-derived nanobody against KLH and its preparation and application. The invention involves immunizing the black-browed rat snake with KLH, extracting total RNA from its splenic lymphocytes, constructing a phage display VH gene library, and, after multiple rounds of screening, obtaining nanobody sequences that specifically bind to KLH, such as KLH-1 and KLH-2. This nanobody can be efficiently and solublely expressed and purified in *E. coli*, exhibiting characteristics of small molecular weight and high affinity. This invention further provides applications for this nanobody, particularly in the preparation of KLH-targeting detection reagents or drug delivery systems. This nanobody provides a core tool molecule for developing highly sensitive KLH-targeting detection reagents, efficient drug delivery systems, and novel vaccines.

The invention discloses an immune effect evaluation method of a cysticercus cellulosae recombinant TPx protein vaccine, and belongs to the technical field of biological medicines. The vaccine takes recombinant thioredoxin peroxidase (TPx) from cysticercus cellulosae as an immunogen, and an adjuvant (such as a Freund's adjuvant) can be optionally supplemented to enhance the immune effect. A mouse is immunized through subcutaneous injection of the vaccine, it is proved that the vaccine can effectively induce an organism to generate humoral immune response and cellular immune response, specifically, the levels of serum IgG, IgG2a and IgG1 antibodies are remarkably increased, the proliferation capacity of splenic lymphocytes is enhanced, and the secretion levels of Th1 type cell factors (TNF-alpha and IL-12) and Th2 type cell factors (IL-13 and IL-10) are remarkably increased. The invention provides a new candidate vaccine for prevention of cysticercosis, and has important application value.

The invention discloses application of radix trichosanthis polysaccharide to preparation of a medicine for regulating immunity and inhibiting postoperative recurrence of colorectal cancer. The radix trichosanthis polysaccharide is found to be capable of promoting M2 phenotype macrophages to be polarized to M1 phenotype, and has an immunoregulation function; meanwhile, proliferation of peripheral blood lymphocytes and spleen lymphocytes is promoted, the proportion of CD4 +, CD8 + and NK cells is increased, and the effect of enhancing immunity is achieved. Furthermore, the hydrogel CMOT prepared by taking the radix trichosanthis polysaccharide as the raw material has good biocompatibility in vitro and in vivo and has the qualified characteristic of hydrogel, and the CMOT hydrogel is found to have the effect of inhibiting the recurrence of the colorectal cancer when being applied to the resection part of the mouse colorectal cancer operation. Therefore, the radix trichosanthis polysaccharide hydrogel has the potential of becoming a postoperative wound dressing after cancer resection.

The invention provides an anti-human CD8 rabbit recombinant monoclonal antibody and application thereof. The antibody comprises a heavy chain variable region VH and a light chain variable region VL, a rabbit is immunized with a KLH-coupled CD8 polypeptide antigen, spleen lymphocytes are separated after titer detection is qualified, and single B cell sorting is carried out through a biotin-labeled antigen; positive clone is obtained by a rabbit single B cell culture technology, RNA (Ribonucleic Acid) is extracted and is reversely transcribed into cDNA (Complementary Deoxyribonucleic Acid), and light and heavy chain variable region genes are amplified and cloned into an expression vector for recombinant expression; and finally, the rabbit recombinant monoclonal antibody with high affinity is obtained. The antibody has the capacity of specifically recognizing CD8 + cells in human peripheral blood, is high in affinity and low in animal origin, is suitable for immunodetection such as flow cytometry and ELISA (enzyme-linked immunosorbent assay), and can be used in the fields of immune cell typing, immune function evaluation, immunotherapy monitoring and the like. The invention also provides a light and heavy chain variable region amino acid sequence of the antibody, and a basis is provided for intellectual property protection and further development of the antibody.

The application discloses a Rhododendron molle flower extract with immunosuppressive activity and a preparation method and application thereof. The Rhododendron molle flower extract is prepared by taking dried flower powder of Rhododendron molle as raw material and 95% ethanol as an extracting agent under the following conditions: condensation reflux extraction for 2 hours at 60 DEG C and a material-liquid ratio of 1:10. The extract is filtered and collected, and the above extract is combined and concentrated under vacuum and reduced pressure to obtain the Rhododendron molle flower extract. The extract has an inhibitory rate of 61.41% on T lymphocyte proliferation induced by abrin and an inhibitory rate of 51.54% on B lymphocyte proliferation induced by lipopolysaccharide at a concentration of 100 mg / mL, and has no cytotoxicity on normal mouse spleen lymphocytes. The application provides a candidate research material for researching and developing a new immunosuppressant, and provides a scientific basis for developing and utilizing the Rhododendron molle resources.

The invention provides a key active peptide component of spleen polypeptide as well as a fine separation method and application thereof, and belongs to the technical field of protein and active peptides, the fine separation method comprises the following steps: separating out the key active peptide component of the spleen polypeptide from spleen polypeptide trapped fluid with the molecular weight of 1-3kDa by adopting a gel chromatography separation method; the key active peptide component of the spleen polypeptide is used for preparing an immunomodulator, an anti-tumor drug, a tumor postoperative rehabilitation drug or a drug for inhibiting tumor metastasis. It is found that PP-VIII polypeptide with the ultraviolet absorption peak being 258.5 nm is a key active peptide component, the bidirectional immunoregulation activity of the PP-VIII polypeptide is remarkably higher than that of other polypeptides, and meanwhile splenic lymphocyte proliferation and the phagocytic ability of macrophages can be remarkably promoted; the traditional Chinese medicine composition has a very remarkable effect on the recovery of the immunocompetence and the weight of immunosuppressed mice and the index improvement of organs, and has a remarkable inhibition effect on tumors of nude mice bearing melanoma, and the inhibition rate reaches 85% or above.

The invention provides a spleen polypeptide with the molecular weight of 1-3 kDa and a preparation method and application thereof, and belongs to the technical field of protein and active peptides, and the spleen polypeptide with the molecular weight of 1-3 kDa is used for preparing an immunomodulator. And / or the spleen polypeptide with the molecular weight of 1-3kDa is used for preparing an anti-tumor drug or a tumor postoperative rehabilitation drug; according to the preparation method, a spleen polypeptide enzymolysis stock solution is taken as a raw material, and the spleen polypeptide with the molecular weight of 1-3kDa is separated by a membrane combination method. The spleen polypeptide with the molecular weight of 1-3kDa has remarkable bidirectional immunomodulatory activity, and can enhance the immune function of an immunosuppressive mouse, including improving the index of immune organs, promoting the proliferation of splenic lymphocytes, enhancing the phagocytic ability of macrophages and the like; meanwhile, the compound has a remarkable inhibition effect on tumor growth of nude mice bearing melanoma, and the inhibition rate reaches 80% or above.

The invention relates to the technical field of biological medicine, in particular to a novel application of a burdock-fructus forsythiae medicine pair and a medicine containing the burdock-fructus forsythiae medicine pair and used for adjusting the hormone level, and finds that the burdock-fructus forsythiae medicine pair can improve the hormone level of female mice and male rats for the first time. Tests finally find that when the mass ratio of the burdock alcohol extract to the fructus forsythiae alcohol extract is 1: 2, the hormone level adjusting effect is optimal, the inhibition effect of cyclophosphamide on splenic lymphocytes can be effectively relieved, meanwhile, the anti-inflammatory effect is optimal, and the pharmaceutical composition is suitable for clinical application. Therefore, the effect of treating the acne can be achieved by independently using the burdock alcohol extract and the fructus forsythiae alcohol extract, and the problems that a traditional Chinese medicine composition is complex in compatibility, high in medication cost, large in side effect and the like are solved.

The invention discloses a preparation method of an insect protein and edible fungus polysaccharide compound and application of the insect protein and edible fungus polysaccharide compound in functional food, and belongs to the technical field of food biology.According to the method, a supercritical extraction technology is adopted for extracting tenebrio molitor protein, and the extraction rate is larger than 90%; the morchella polysaccharide is extracted by adopting an ultrasonic-assisted extraction technology, and the extraction rate is 12-18%; protein and polysaccharide are combined through an electrostatic self-assembly technology, a nanoscale compound with the average particle size of 200-250 nm is formed, the compound has remarkable immunoregulation and antioxidant activity, the promoting effect on splenic lymphocyte proliferation is improved by 1.8-2.3 times compared with that of a single component, the bioavailability is improved by 2.8 times or above, and the preparation method is simple and easy to implement. The method can be applied to preparation of functional foods such as oral liquid and capsules.